Granular vulvovaginitis (nodular venereal disease) of cattle associated with Mycoplasma bovigenitalium

Afshar, A; Stuart, Patrick M.; Huck, R.A.

doi:10.1136/vr.78.15.512

Cited by 34 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Treatment of the sera with rivanol removed the SA, anti-y-globulin, CF homologous and CF heterologous activities. These results differed from those obtained with serum samples taken 2 years previously from a cow, 3 weeks after infection with the M 120 strain of M. bovigenitalium (Afshar et al 1966): antibody activity was found both in the 7 S and 19 S fractions, with most of the activity in the 7 S fraction. The rivanol and mercaptoethanol treatments confirmed these results.…”

Section: Resultscontrasting

confidence: 56%

“…The infected quarters became swollen and tender, while the uninfected quarters remained unchanged. The clinical signs and secretions of the affected quarters were characteristic of M. bovigenitalium mastitis (Stuart et al 1963;Afshar, Stuart & Huck, 1966). By the 17th day after inoculation, the infected quarters of cows C and D had recovered from the infection and the appearance of the milk was normal.…”

Section: Resultsmentioning

confidence: 92%

See 1 more Smart Citation

Immunological response in cows toMycoplasma bovigenitalium, strain 1836

Roberts

1968

Epidemiol. Infect.

View full text Add to dashboard Cite

Four cows were inoculated via the udder with the 1836 strain of Mycoplasma bovigenitalium. The serological response was investigated using the complement-fixation and serum plate agglutination tests. A heterologous response to M. mycoides was observed, associated with an increase in the anti-γ-globulin titres in the cow's serum. The heterologous response persisted in one cow for 6 weeks after infection. The nature of the immunoglobulin response was characterized by density gradient centrifugation, and by mercaptoethanol and rivanol treatment of the serum and was found to consist entirely of 19 S γ-globulins.The Mycoplasma persisted in the udder for the 18 weeks duration of the experiment. The Mycoplasma was not isolated from the blood. One of the cows became lame with swollen joints; Bacillus cereus was isolated from the joints and blood.

show abstract

Section: Resultscontrasting

confidence: 56%

Section: Resultsmentioning

confidence: 92%

Immunological response in cows toMycoplasma bovigenitalium, strain 1836

Roberts

1968

Epidemiol. Infect.

View full text Add to dashboard Cite

show abstract

“…The M 120 strain of Mycoplasma bovigenitalium (Afshar et al 1966) was used throughout the experiments, the strain had previously been passaged twice in calf-kidney cell cultures (M 120 CK2). A stock suspension of M 120 was prepared by collecting the supernatant after freezing and thawing and light centrifugation (1 74g for 5 min.).…”

Section: Methodsmentioning

confidence: 99%

“…of varying type and degree (Hayfbck & Stinebring, 1960;Nelson, 1960;Casterjon-Diez, Fisher & Fisher, 1963 ;ROVOZZO, Luginbuhl & Helmboldt, 1963;Grumbles, Hall & Cummings, 1964;Butler & Leach, 1964). During the course of investigation of outbreaks of bovine infertility strain M 120 of Mycoplasma bovigenitalium was isolated from vaginal swabs obtained from a heifer showing lesions of granular vulvovaginitis (Afshar, Stuart & Huck, 1966). This paper gives a detailed account of the growth and cytopathic effect of this strain in tissue-culture systems.…”

Section: Introductionmentioning

confidence: 99%

The Growth of Mycoplasma bovigenitalium in Cell Cultures

Afshar

1967

Journal of General Microbiology

Self Cite

View full text Add to dashboard Cite

SUMMARYA strain of Mycoplasma bovigenitalium, designated M 120, was grown, and produced a marked cytopathic effect (c.p.e.) in calf-, pig-, and monkeykidney cell cultures. The c.p.e. was characterized by enlargement of the cells, the appearance of intracytoplasmic inclusions and partial destruction of the cell monolayers. A similar c.p.e. was produced in tissue-culture cells following the inoculation of mycoplasma 'toxins'.Comparative growth studies of strain M 120 in calf-kidney cell cultures and in tissue-culture medium alone showed that the organism grew more readily in the presence of cells. The rate of virus production and appearance of c.p.e. of infectious bovine rhinotracheitis (I.B.R.) virus was delayed in cultures previously infected with the M 120 strain of mycoplasma as compared with normal cultures.

show abstract

“…Mycoplasma serogroup 11 induces vulvovaginitis, cervicitis, endometritis, epididymitis and oophoritis (Ruffin, 2001), is pathogenic to the udder of lactating sheep and produces marked biochemical alterations in the milk (Kumar et al, 1993;Rana et al, 1993). M. bovigenitalium is commonly isolated from the reproductive tracts of cattle and buffaloes and some strains are implicated in mastitis, arthritis, balanoposthitis and genital discards (Afshar et al, 1966;Jackson & Boughton, 1991;Razin & Freundt, 1984;Thiede et al, 2002). Variations in virulence in bovine udder infections exist for different strains of M. bovigenitalium (Mackie & Ball, 1984): a`mastitis strain', isolated from an abnormal dry udder secretion, was highly pathogenic, and a`vaginal strain', isolated from the vaginal mucus of a cow after abortion, produced a low level infection.…”

mentioning

confidence: 99%

Proposal that the strains of the Mycoplasma ovine/caprine serogroup 11 be reclassified as Mycoplasma bovigenitalium

Nicholas

Lin

Sachse

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

This proposal is our response to the recommendation of the International Committee on Systematics of Prokaryotes (Subcommittee on the taxonomy of Mollicutes) that we`write a proposal to classify Mycoplasma bovigenitalium and ovine/caprine serogroup 11 as a single species'. Physiological and phylogenetic comparisons between 27 strains of M. bovigenitalium and Mycoplasma serogroup 11 showed that (i) growth and patterns of organic acid substrate use completely overlapped among strains; (ii) all had lipase and phosphatase activities; (iii) the strains were indistinguishable in their SDS±PAGE whole-cell protein profiles, which differed from five other species; (iv) strains were indistinguishable in immunoblotting of cell proteins and cross-reactivity in ELISA, but differed from other Mycoplasma species; (v) DNA±DNA hybridization did not distinguish between the two groups, and (vi) comparison of 16S and 23S rRNA gene sequences of ten strains of Mycoplasma serogroup 11 and six strains of M. bovigenitalium showed that they shared 98±100 % similarity across all strains tested, but only 86±95 % to other Mycoplasma species. Strains of the Mycoplasma ovine/caprine serogroup 11 must therefore be reassigned as Mycoplasma bovigenitalium.

show abstract

Granular vulvovaginitis (nodular venereal disease) of cattle associated with Mycoplasma bovigenitalium

Cited by 34 publications

References 0 publications

Immunological response in cows toMycoplasma bovigenitalium, strain 1836

Immunological response in cows toMycoplasma bovigenitalium, strain 1836

The Growth of Mycoplasma bovigenitalium in Cell Cultures

Proposal that the strains of the Mycoplasma ovine/caprine serogroup 11 be reclassified as Mycoplasma bovigenitalium

Contact Info

Product

Resources

About