Granule-bound starch synthase cDNA in Chlorella kessleri 11 h: cloning and regulation of expression by CO2 concentration

Oyama, Yasunori; Izumo, Asako; Fujiwara, Shoko; Shimonaga, Takahiro; Nakamura, Yasunori; Tsuzuki, Mikio

doi:10.1007/s00425-006-0239-7

Cited by 16 publications

(3 citation statements)

References 37 publications

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“…The amylose content (19.1%DW) and amylose concentration (449 mg L −1 ) under −N on Day 2 in the culture with the addition of 1 g L −1 NaHCO 3 (Tables 1 and 2) were 2.4- to 3.5-fold of those obtained in Chlorella sorokiniana [41]. The amylose content in total starch reached 33%, which was comparable to C. reinhardtii under nitrogen deprivation with mixotrophic cultivations, or 18% higher than Chlorella with photoautotrophic cultivations [37, 40]. This amylose level was even higher than most of the starch from native cereal crops where amylose accounts for about 15–32% of storage starch [47].…”

Section: Resultsmentioning

confidence: 94%

“…This phenomenon, to the best of our knowledge, was rarely reported previously, the mechanism of which was poorly understood either. In fact, the enhanced Am proportion in TS was also observed in Chlorella under low CO 2 (air, 0.038%) conditions where CO 2 -concentrating mechanisms (CCM) was induced to synthesize a pyrenoidal starch sheath [40, 41]. The addition of bicarbonate herein should also induce a CA-mediated CCM for carbon utilization [11].…”

Section: Resultsmentioning

confidence: 99%

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Application of an in situ CO2–bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis

Yao

Sun

et al. 2019

Biotechnol Biofuels

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Background Microalgal starch is regarded as a promising alternative to crop-based starch for biorefinery such as the production of biofuels and bio-based chemicals. The single or separate use of inorganic carbon source, e.g., CO 2 and NaHCO 3 , caused aberrant pH, which restricts the biomass and starch production. The present study applied an in situ CO 2 –NaHCO 3 system to regulate photosynthetic biomass and starch production along with starch quality in a marine green microalga Tetraselmis subcordiformis under nitrogen-depletion (−N) and nitrogen-limitation (±N) conditions. Results The CO 2 (2%)–NaHCO 3 (1 g L −1 ) system stabilized the pH at 7.7 in the −N cultivation, under which the optimal biomass and starch accumulation were achieved. The biomass and starch productivity under −N were improved by 2.1-fold and 1.7-fold, respectively, with 1 g L −1 NaHCO 3 addition compared with the one without NaHCO 3 addition. NaHCO 3 addition alleviated the high-dCO 2 inhibition caused by the single CO 2 aeration, and provided sufficient effective carbon source HCO 3 − for the maintenance of adequate photosynthetic efficiency and increase in photoprotection to facilitate the biomass and starch production. The amylose content was also increased by 44% under this CO 2 –bicarbonate system compared to the single use of CO 2 . The highest starch productivity of 0.73 g L −1 day −1 under −N cultivation and highest starch concentration of 4.14 g L −1 under ±N cultivation were both achieved with the addition of 1 g L −1 NaHCO 3 . These levels were comparable to or exceeded the current achievements reported in studies. The addition of 5 g L −1 NaHCO 3 under ±N cultivation led to a production of high-amylose starch (59.3% of total starch), which could be used as a source of functional food. Conclusions The in situ CO 2 –NaHCO 3 system significantly improved the biomass and starch production in T. subcordiformis . It could also regulate the starch quality with varied relative amylose content under different cultivation modes for diverse downstream applications that could promote the economic feasibility of microalgal starch-based biofuel production. Adoption of this system in T. subcordiformis would faci...

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Application of an in situ CO2–bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis

Yao

Sun

et al. 2019

Biotechnol Biofuels

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“…On the other hand, the Euglenophyta, Heterokontophyta, and Haptophyta storepolyglucans called paramylon (Euglenophyta), laminaran (Phaeophyceae), and chrysolaminaran (Bacillariophyceae and Haptophyta). Chrysolaminaran of the Bacillariophyceae comprises -(1!3)-polyglucans with occasional branching at the C-2 and C-6 positions of glucose (Paulsen and Myklestad 1978;McConville et al 1986), and its degree of polymerization (DP) is about 16-60 (about 2.5-10 kDa) (Craigie 1974;Paulsen and Myklestad 1978), which is much lower than those of starch (10 2 -10 6 kDa) and paramylon (more than 500 kDa) (Clark and Stone 1960;Ball 1998;Oyama et al 2006). Chrysolaminaran is water-soluble and localized in vacuoles (Waterkeyn and Bienfait 1987).…”

Section: Introductionmentioning

confidence: 99%

Structural and physiological studies on the storage β-polyglucan of haptophyte Pleurochrysis haptonemofera

Hirokawa

Fujiwara

Suzuki

et al. 2007

Planta

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The storage beta-polyglucan and catabolic enzyme activities of the haptophyte Pleurochrysis haptonemofera were characterized. The storage beta-polyglucan was prepared by the dimethylsulfoxide-extraction method. (13)C- and (1)H-NMR spectroscopy revealed that the polyglucan consists of beta-(1-->3)- and beta-(1-->6)-linked glucose polymers, with a beta-(1-->6)- to beta-(1-->3)-linkage ratio of 1.5. Gel permeation chromatography showed that the molecular weight of the polyglucan is 1.1-8.4 x 10(4) Da, with a peak at 3.4 x 10(4) Da. The degree of polymerization, which was estimated from the amounts of total carbohydrate and reduced ends, was 203, corresponding to 3.3 x 10(4) Da. A method for measurement of the beta-polyglucan in a small amount of liquid culture involving a mixture of beta-glucanases, Westase, was established. The beta-polyglucan was localized in the soluble fraction of cells. The amount of beta-polyglucan per cell increased at the stationary phase under continuous illumination and decreased in the dark, like those of storage alpha-polyglucans, starch of green algae and glycogen of cyanobacteria. The activities of beta-1,3- and beta-1,6-glucanases involved in the degradation of the storage beta-polyglucan were assayed in vitro, both being optimal at pH 5.0. The beta-1,3-glucanase activity, which was detected on active staining after native polyacrylamide gel electrophoresis, was partially purified by ammonium sulfate precipitation and anion exchange chromatography.

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Carbon‐concentrating mechanism in a green alga, Chlamydomonas reinhardtii, revealed by transcriptome analyses

Yamano

Fukuzawa

2009

J. Basic Microbiol.

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Aquatic photosynthetic organisms can acclimate to the variable and limiting availability of CO(2) by operation of carbon-concentrating mechanism (CCM) that allows them to optimize carbon acquisition for photosynthesis. The CCMs of both eukaryotic alga and cyanobacteria function to facilitate CO(2) assimilation, when inorganic carbon (Ci; CO(2) and/or HCO(3)(-)) is limited. By active Ci uptake systems, internal Ci levels are increased and then carbonic anhydrase supplies sufficient CO(2) to ribulose 1,5-bisphosphate carboxylase/oxygenase by the dehydration of accumulated bicarbonate. Although the molecular components of CCM have been intensively studied in cyanobacteria, significant advances in understanding of the eukaryotic CCM have been achieved especially in a model green alga, Chlamydomonas reinhardtii. Recent accumulation of genomic sequence data of algae leads to start comparative genomic analyses of functional components of eukaryotic CCM. This review focuses on the recent advances in identifying and characterizing the components of the CCM by transcriptome analyses of the Chlamydomonas cells that are transferred to CO(2)-limiting stress conditions in light.

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Granule-bound starch synthase cDNA in Chlorella kessleri 11 h: cloning and regulation of expression by CO2 concentration

Cited by 16 publications

References 37 publications

Application of an in situ CO2–bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis

Application of an in situ CO2–bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis

Structural and physiological studies on the storage β-polyglucan of haptophyte Pleurochrysis haptonemofera

Carbon‐concentrating mechanism in a green alga, Chlamydomonas reinhardtii, revealed by transcriptome analyses

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