Granulocytes and cultured human fibroblasts express common acute lymphoblastic leukemia-associated antigens

Braun, MP; Petřek, Martin; Ledbetter, J A; Hansen, JA

doi:10.1182/blood.v61.4.718.bloodjournal614718

Cited by 15 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This interpretation is in line with a number of studies performed by different groups (45)(46)(47)(48)(49). It has been known for some years (45,46) that various CALLA-specific McAb, including the J5 employed here, positively stain the surface of mature granulocytes and are able to precipitate an antigen of a slightly higher molecular weight (95-1 10 KD) from their surface than has been isolated from lymphoid cells (95-100 KD). More recently, two laboratories (47,48) clearly demonstrated that human granulocytes synthesize CALLA in vitro.…”

Section: Reactivity Of the Anti-cdzo Mcab Bi And If5 With Aml Cells Isupporting

confidence: 89%

Immunological typing of acute leukemias: Immunoenzymatic staining of fixed cells compared with immunofluorescence staining of unfixed cells in suspension

Ly¹,

Beiske

Larsen³

1988

European J of Haematology

View full text Add to dashboard Cite

A panel of 14 monoclonal antibodies (McAb) against hematopoietic cell surface antigens was applied on mononuclear blood or bone marrow cells from 40 cases of acute leukemia in order to compare immunoenzymatic staining (IE) (alkaline phosphatase) of fixed cells with immunofluorescence staining (IF) of unfixed suspended cells. According to the immunological results, 25 cases were phenotyped as ALL and 15 cases as AML. Cases with blast crisis secondary to chronic myelogeneous leukemia (CML‐BC) were not represented in this series. In all ALL cases the two methods gave an identical antigenic distribution. In 20 our of 21 cases of non‐T‐cell ALL, a B‐cell progenitor origin was demonstrated by a positive staining reaction with the anti‐CD 19 McAb AB1 or HD37, and in 10 cases additionally with the anti‐CD20 McAb B1 or 1F5. In contrast to the results obtained with IF, IE revealed a poor preservation of the AB1 epitope on CD19, whereas the HD37 epitope was equally well demonstrated by both methods. In 15 cases of AML the distribution of positive versus negative cells with IE or IF was identical for all McAb except J5 (anti‐CALLA) (CD10) and Bl (CD20). Thus, 10/15 AML cases expressed CALLA with IE compared to 2/15 with IF. The corresponding figures for Bl were 5/15 and 0/15, respectively. Accordingly, normal myeloid precursor cells were CALLA‐positive with IE but negative with IF. The discrepancy probably reflects the fact that, whereas both intracytoplasmatic and membrane‐bound antigens are exposed in IE, only the latter are in IF. If the alteration of antigenic accessibility after fixation is considered, IE can safely be used for immunophenotyping of acute leukemia.

show abstract

Section: Reactivity Of the Anti-cdzo Mcab Bi And If5 With Aml Cells Isupporting

confidence: 89%

Immunological typing of acute leukemias: Immunoenzymatic staining of fixed cells compared with immunofluorescence staining of unfixed cells in suspension

Ly¹,

Beiske

Larsen³

1988

European J of Haematology

View full text Add to dashboard Cite

show abstract

“…30,33 However, it may be said that CD10 expression had no relationship with histiocytic differentiation in this case. 34,35 In short, this case of cellular neurothekeoma with histiocytic differentiation could be explained by the following three interpretations: (1) plexiform and epithelioid variants of dermatofibroma, (2) cellular neurothekeoma with immature nerve sheath differentiation incidentally expressing histiocytic markers, and (3) cellular neurothekeoma as an undifferentiated neoplasm derived from neural crest cells of nerve sheath/ fibrohistiocyte lineage. We prefer the latter two views because of the concomitant expressions of PGP9.5/ S-100A6 and PG-M1/CD68 (KP-1).…”

Section: Discussionmentioning

confidence: 93%

Cellular neurothekeoma with histiocytic differentiation

Misago¹,

Satoh

Narisawa³

2004

J Cutan Pathol

View full text Add to dashboard Cite

Background: It is generally accepted that the two types of neurothekeoma (myxoid type and cellular type) represent the two poles of a spectrum. This concept, however, has recently been challenged, and cellular neurothekeomas have been suggested as a separate classification and are included in the ‘fibrohistiocytic’ category by some authors. Cellular neurothekeomas have been reported to show negative immunohistochemical staining for histiocytic markers, and PG‐M1 is now considered to be the most reliable histiocytic marker. Case report: We report a case of cellular neurothekeoma. The histopathological features in this case were typical for cellular neurothekeoma. Immunohistochemically, the neoplastic cells were diffusely positive for S‐100A6 protein, PGP9.5, CD10, CD68 (KP1), PG‐M1, and Vimentin, and negative for other antibodies including S‐100 protein and factor XIIIa. Conclusions: Cellular neurothekeoma expressing both KP‐1 and PG‐M1 is considered to show histiocytic differentiation, and may be interpreted as a neoplasm with immature nerve sheath differentiation, incidentally expressing histiocytic markers, or as an undifferentiated neoplasm derived from the neural crest cells of nerve sheath/fibrohistiocyte lineage. These results, such as the concomitant expressions of PGP9.5/S‐100A6 and PG‐M1/CD68 (KP‐1), support the theory of multiple differentiation in cellular neurothekeomas. The significance of the expression of CD10 in this cellular neurothekeoma is unclear.

show abstract

“…Tumor cell lines such as melanomas (Carrel et al, 1983) and gliomas (Carrel et al, 1982) express CALLA, as detected by monoclonal antibodies (MAbs). This antigen has also been localized in several normal tissues such as kidney (Metzgar et al, 1981) and neutrophils (Braun et al, 1983).…”

mentioning

confidence: 96%

Degradation of alpha‐melanocyte stimulating hormone (alpha‐MSH) by CALLA/endopeptidase 24. 11 expressed by human melanoma cells in culture

Deschodt-Lanckman

Vanneste

Loir

et al. 1990

Intl Journal of Cancer

View full text Add to dashboard Cite

The common acute lymphoblastic leukemia antigen (CALLA) is identical to human endopeptidase 24.11 (E-24.11) and is expressed on certain human melanoma lines. This work was conducted in order to investigate whether alpha-melanocyte-stimulating hormone (alpha-MSH) could be a substrate for E-24.11, its degradation leading to the negative alpha-MSH radiobinding assay results observed with some CALLA-positive cell lines. We used 3 human melanoma cell lines (GLL-19, Mel Juso and G361) which lack receptors to alpha-MSH and express CALLA, and, as a control, one CALLA-negative melanoma cell line (HBL) with specific receptors for alpha-MSH. Radioimmunoassays give evidence that alpha-MSH was degraded in the presence of the 4 melanoma cell lines and that disappearance of the peptide was significantly reduced by phosphoramidon in 2 lines (GLL-19 and G361). Upon incubation of alpha-MSH with GLL-19 and G361 cell membranes, 3 degradation products were completely abolished in the presence of phosphoramidon. Amino acid content analysis of alpha-MSH fragments produced by purified E-24.11 permitted identification of 6 peptide bonds in the sequence of alpha-MSH susceptible to cleavage by the enzyme. It is concluded that alpha-MSH is a substrate in vitro for purified E-24.11 and for the enzyme present on the human melanoma cell lines GLL-19 and G361, expressing a high level of endopeptidase activity. However, hydrolysis of alpha-MSH by this enzyme does not seem to represent the main factor responsible for the apparent absence of receptors for the hormone on some cell lines.

show abstract

Granulocytes and cultured human fibroblasts express common acute lymphoblastic leukemia-associated antigens

Cited by 15 publications

References 0 publications

Immunological typing of acute leukemias: Immunoenzymatic staining of fixed cells compared with immunofluorescence staining of unfixed cells in suspension

Immunological typing of acute leukemias: Immunoenzymatic staining of fixed cells compared with immunofluorescence staining of unfixed cells in suspension

Cellular neurothekeoma with histiocytic differentiation

Degradation of alpha‐melanocyte stimulating hormone (alpha‐MSH) by CALLA/endopeptidase 24. 11 expressed by human melanoma cells in culture

Contact Info

Product

Resources

About