Granulomatous rhinitis associated with Pythium insidiosum infection in sheep

Abstract

The rhinitis and granulomatous lymphadenitis was characterised histopathologically by caseous necrosis with proliferation of connective tissue, a neutrophilic and eosinophilic infiltrate, epithelioid cells, multinucleate giant cells, and irregular, moderately septate and ramified hyphae, frequently surrounded by abundant granular and eosinophilic material with radial disposition (Splendore-Hoeppli phenomenon). Lesions from the dorsal and lateral regions of the nose were collected aseptically and transported to the laboratory at room temperature. The samples were placed on to 2 per cent Sabouraud's dextrose agar and incubated at 37°C. Forty-eight hours later, the cultures demonstrated a slight growth of white colonies with submerged root-like elements. Later, it was possible to observe organisms morphologically compatible with P insidiosum following the induction of zoosporo genesis, performed as described by Santurio and others (2003). The identity of the pathogen was confirmed in all four cases through the amplification of DNA by PCR, as described by Rodrigues and others (2006). The extensive ulcerative lesions observed in the sheep resembled the cutaneous lesions described in sheep by Tabosa and others (2004), in horses by Alfaro and Mendoza (1990), in dogs by Thomas and Lewis (1998) and in cattle by Santurio and others (1998). The histopathological findings of granulomatous eosinophilic lesions with many giant cells and Splendore-Hoeppli material involving P insidiosum hyphae are consistent with those observed in previous studies involving sheep from northeastern Brazil (Tabosa and

Pythiosis in sheep from Paraná, southern Brazil

“…In sheep, the ulcerative cutaneous form (Tabosa et al 2004) has been verified, but metastasis can develop as well, affecting lymph nodes and lungs (Souza et al 2008). Recently, cases of granulomatous rhinitis were verified (Santurio et al 2008;Souza et al 2008, Portela et al 2010, Carrera et al 2013, with the destruction of the hard palate, and reaching the nasal vestibule, resulting in a fistula. Microscopically, it is characterized by the development of granulomatous lesions, where it is possible to observe necrotic tissue, Splendore-Hoeppli phenomenon and inflammatory infiltration (Carrera et al 2013).…”

Aspectos epidemiológicos da conidiobolomicose em ovinos na região semiárida do Nordeste do Brasil

“…Os diagnósticos de pitiose rinofacial ou conidiobolomicose foram realizados com base nas características clínicas (SILVA et al, 2007a;RIET-CORREA et al, 2008;BOABAID et al, 2008;SANTURIO et al 2008;PEDROSO et al, 2009;FURLAN et al, 2010;PORTELA et al, 2010;UBIALI et al, 2013). Além de constatar as características clínicas da doença, foram necropsiados 22 ovinos, nos quais foram realizados estudos histológicos.…”

“…Rinites granulomatosas causadas por fungos da Classe Zygomycetes, incluindo Conidiobolus lamprauges (RIET-CORREA et al, 2008;DE PAULA et al, 2010;VILELA et al, 2010) ou por Pythium insidiosum, um oomiceto do Reino Straminipila (SANTURIO et al, 2008), são frequentes em ovinos. Conidiobolus spp.…”

“…têm sido descritos no Piauí (SILVA et al, 2007a), Paraíba (RIET-CORREA et al, 2008), Mato Grosso (BOABAID et al, 2008;SILVEIRA et al, 2013), Rio Grande do Sul (PEDROSO et al, 2009) e Santa Catarina (FURLAN et al, 2010). Já focos de rinite rinofacial causados por P. insidiosum foram relatados no Mato Grosso (SANTURIO et al, 2008;UBIALI et al, 2013) e Paraíba (RIET-CORREA et al, 2008;PORTELA et al, 2010). Esses trabalhos descrevem, principalmente, os aspectos clínicos e patológicos da conidiobolomicose e da pitiose rinofacial, porém são escassos os dados sobre a epidemiologia dessas enfermidades.…”

Rhinocerebral and rhinopharyngeal conidiobolomycosis in sheep

“…In Northeastern Brazil, a differential diagnose must be made from a disease promoting very similar clinical signs as granulotous rhinitis promoted by Pythium insidiosum (SANTURIO et al, 2008), whilst enzootic nasal adenocarcinoma of sheep is diagnosed in many countries of Europe (SVARA et al, 2006). The isolation of C. coronatus was crucial to confirm the presumptive diagnosis.…”