Grape seed extract has superior beneficial effects than vitamin E on oxidative stress and apoptosis in the hippocampus of streptozotocin induced diabetic rats

Yonguc, Goksin Nilufer; Dodurga, Yavuz; Adıgüzel, Esat; Gündoğdu, Gülşah; Küçükatay, Vural; Özbal, Seda; Yılmaz, İsmail; Cankurt, Ulker; Yılmaz, Yusuf; Akdoğan, Ilgaz

doi:10.1016/j.gene.2014.10.052

Cited by 56 publications

(44 citation statements)

References 65 publications

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“…Oxidative stress was thought to be one pathological factor for cell death. The antioxidants, such as grape seed extract and vitamin E were also reported to mitigate hippocampal cell loss in diabetic model [24]. In addition, SFN also possibly ameliorated the memory impairment in diabetic model through anti-inflammation [25].…”

Section: Discussionmentioning

confidence: 96%

Sulforaphane Prevents Neuronal Apoptosis and Memory Impairment in Diabetic Rats

Wang

Fang

Zhen

et al. 2016

Cell Physiol Biochem

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Background/Aims: To explore the effects of sulforaphane (SFN) on neuronal apoptosis in hippocampus and memory impairment in diabetic rats. Methods: Thirty male rats were randomly divided into normal control, diabetic model and SFN treatment groups (N = 10 in each group). Streptozotocin (STZ) was applied to establish diabetic model. Water Morris maze task was applied to test learning and memory. Tunel assaying was used to detect apoptosis in hippocampus. The expressions of Caspase-3 and myeloid cell leukemia 1(MCL-1) were detected by western blotting. Neurotrophic factor levels and AKT/GSK3β pathway were also detected. Results: Compared with normal control, learning and memory were apparently impaired, with up-regulation of Caspase-3 and down-regulation of MCL-1 in diabetic rats. Apoptotic neurons were also found in CA1 region after diabetic modeling. By contrast, SFN treatment prevented the memory impairment, decreased the apoptosis of hippocampal neurons. SFN also attenuated the abnormal expression of Caspase-3 and MCL-1 in diabetic model. Mechanically, SFN treatment reversed diabetic modeling-induced decrease of p-Akt, p-GSK3β, NGF and BDNF expressions. Conclusion: SFN could prevent the memory impairment and apoptosis of hippocampal neurons in diabetic rat. The possible mechanism was related to the regulation of neurotropic factors and Akt/GSK3β pathway.

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Section: Discussionmentioning

confidence: 96%

Sulforaphane Prevents Neuronal Apoptosis and Memory Impairment in Diabetic Rats

Wang

Fang

Zhen

et al. 2016

Cell Physiol Biochem

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“…GSE, as a powerful free-radical scavenger, has recently received much attention due to its broad spectrum of pharmacological properties against OS (32). Many of the reported therapeutic effects of GSE reside in its high content of flavonoids and phenolic acids (14). Recently, a substantial body of growing evidence has suggested that GSE inhibits oxidative injury through modulation of antioxidant enzyme system expression and the suppression of nitrosative/oxidative stress (33,34).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, at the testis level, OS is involved in steroidogenesis impairment and germinal epithelium differentiation disruption (9,10). Growing evidence demonstrates that the black grape (Vitis vinifera) and its seed possess a broad spectrum of pharmacological properties, including free-radical scavenging (11), antitumor (12), antifibrotic (13), anti-apoptotic (14), and anti-inflammatory activities (15). Moreover, recent studies have revealed that grape seed extract (GSE) has a potential reproprotective effect in male reproductive disorders (16,17).…”

Section: Introductionmentioning

confidence: 99%

Black Grape Seed Extract Modulates Fluoxetine-Induced Oxidative Stress and Cytotoxicity in the Mouse Testis

Hajizadeh

Mehranjani

Najafi

et al. 2016

Jundishapur J Nat Pharm Prod

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Background: Fluoxetine (FLX), a selective serotonin reuptake inhibitor, is widely used in the treatment of neurological disorders. However, its application carries the risk of adverse reactions, including reproductive toxicities. Grape seed is highly regarded for its pharmacological activities, specifically its antioxidant properties. Objectives: This study was designed to evaluate whether hydroalcoholic grape seed extract (GSE) could ameliorate FLX-evoked oxidative injuries in the mouse testis. Materials and Methods: Thirty-two male mice were categorized into four groups of eight mice each. Two groups received FLX at a dose of 20 mg/kg per day over a period of six weeks by oral gavage. One of these groups received GSE at a dose of 100 mg/kg per day orally along with the FLX. An untreated control group and a GSE-only treated group were also included. Serum and testicular samples were collected 24 hours after the last treatment. Results: Biochemical analyses demonstrated that FLX caused significant decreases in testicular antioxidant capacity and serum testosterone concentrations, as well as lipid peroxidation elevations in testicular tissue. Morphometric and histopathological studies supported the biochemical findings. Concomitant administration of GSE with FLX markedly reduced testicular damage and significantly restored all of the mentioned markers to normal values. Conclusions:The findings suggested that oxidative stress-mediated testicular damage, arising as a result of FLX administration in mice, could be reversed by GSE.

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“…Penelitian sebelumnya telah menyatakan bahwa memori, belajar, dan gangguan kognitif lebih umum pada penderita diabetes dibandingkan pasien non diabetes. 4 Hiperglikemia pada DM menyebabkan kenaikan kadar radikal bebas. Proses autooksidasi pada hiperglikemi memicu pembentukan radikal bebas.…”

Section: Pendahuluanunclassified

“…Radikal bebas dapat merusak membran sel, menjadi lipid peroksida atau malondialdehyde (MDA), bila berlanjut mengakibatkan kerusakan sistem membran sel dan kematian sel. 4 Penelitian lain menunjukkan diabetes melitus mengurangi fungsi belajar dan memori tikus, merusak struktur neuron hipokampus dan sinapsis, dan menginduksi apoptosis neuronal yang ditunjukkan dengan meningkatnya aktivitas caspase-3. 5 Caspase adalah keluarga sisteinil protease spesifik aspartat yang terdapat pada organisme multiselular dan berfungsi sebagai regulator sentral apoptosis.…”

Section: Pendahuluanunclassified

PENGARUH ANTOSIANIN UBI UNGU (Ipomoea batatas L.) KULTIVAR GUNUNG KAWI TERHADAP EKSPRESI CASPASE-3 PADA JARINGAN OTAK TIKUS MODEL DM TIPE 2

Prakosa¹,

Ratnawati²,

Prabawati³

2017

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AbstrakDiabetes melitus (DM) merupakangangguan metabolism yang menyebabkan kondisi hiperglikemia. Hiperglikemia akan memicu pembentukan radikal bebas. Radikal bebas yang berlebihan dapat merusak sistem membran sel dan kematian sel di berbagai jaringan tubuh. Salah satu penanda terjadinya kematian sel pada suatu jaringan adalah caspase-3. Ubi ungu merupakan tanaman tradisional yang mengandung antosianin yang bermanfaat sebagai antiinflamasi, antioksidan, neuroprotektan, dan antidiabetik. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian antosianin ubi ungu terhadap ekspresi caspase-3 pada jaringan otak tikus Wistar yang diinduksi DM tipe 2. Penelitian ini menggunakan desain eksperimental post-test only control group. Sampel penelitian terbagi menjadi 5 kelompok yaitu kontrol negatif, kontrol positif, antosianin dosis 10 mg/kgBB, dosis 20 mg/kgBB, dan dosis 80 mg/kgBB selama 5 minggu. Kontrol positif adalah tikus yang dibuat DM tipe 2, sedangkan kontrol negatif adalah tikus sehat. Ekspresi caspase-3 diukur dengan metode imunohistokimia. Analisis statistik menunjukkan bahwa ekspresi caspase-3 berbeda secara bermakna pada semua kelompok perlakuan (ANOVA, p<0,05). Pemberian antosianin dosis 10 dan 20 mg/kgBB menurunkan ekspresi caspase-3 secara bermakna dibandingkan kontrol positif, sedangkan pemberian antosianindosis 80 mg/kgBB justru meningkatkan ekspresi caspase-3. Dapat disimpulkan bahwa pemberian antosianin dosis 10 dan 20 mg/kgBB menurunkan ekspresi caspase-3 pada jaringan otak tikus wistar model DM tipe 2.Kata kunci: antosianin, ekspresi caspase-3, hiperglikemia, jaringan otak THE EFFECT OF PURPLE SWEET POTATO (Ipomoea batatas L.) ANTHOCYANIN ON THE EXPRESSION OF CASPASE-3 IN BRAIN TISSUE OF DIABETES MELLITUS TYPE 2-INDUCED RATS AbstractDiabetes mellitus (DM) is a metabolic disorder that leads to the condition of hyperglycemia. Hyperglycemia will trigger the formation of free radicals. Excessive free radicals can damage the cell membrane system and cell death in various tissues. Caspase-3 is a marker of cell death in a tissue. Purple sweet potato is a traditional crop that contain anthocyanins that are useful as antiinflammatory, antioxidant, neuroprotectant, and antidiabetic. This study was conducted to determine the effect of purple sweet potato anthocyanin on the expression of caspase-3 in brain tissue of diabetes mellitus type 2-induced Wistar rats. This study used an experimental post-test only control group design. The research sample was divided into 5 groups: negative control, positive control, anthocyanin dose of 10 mg/kg, dose of 20 mg/kg, and dose of 80 mg/kg for 6 weeks. Positive control contained of rats with DM type 2, whereas negative control contained of the healthy rats. The expression of caspase-3 was measured by immunohistochemistry methods. Statistic analysis result showed that the expression of caspase-3 increased significantly in all treatment groups (ANOVA, p < 0,05). Anthocyanin dose of 10 and 20mg/kg treatment decreased the expression of caspase-3 significantly more than the...

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Grape seed extract has superior beneficial effects than vitamin E on oxidative stress and apoptosis in the hippocampus of streptozotocin induced diabetic rats

Cited by 56 publications

References 65 publications

Sulforaphane Prevents Neuronal Apoptosis and Memory Impairment in Diabetic Rats

Sulforaphane Prevents Neuronal Apoptosis and Memory Impairment in Diabetic Rats

Black Grape Seed Extract Modulates Fluoxetine-Induced Oxidative Stress and Cytotoxicity in the Mouse Testis

PENGARUH ANTOSIANIN UBI UNGU (Ipomoea batatas L.) KULTIVAR GUNUNG KAWI TERHADAP EKSPRESI CASPASE-3 PADA JARINGAN OTAK TIKUS MODEL DM TIPE 2

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