Grape seed proanthocyanidin extract protects lymphocytes against histone-induced apoptosis

Chang, Ping; Mo, Bing; Cauvi, David M.; Yu, Ying; Guo, Zhenhui; Zhou, Jian; Huang, Qiong; Yan, Qin; Chen, Guiming; Liu, Zhan‐Guo

doi:10.7717/peerj.3108

Cited by 7 publications

(5 citation statements)

References 44 publications

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“…Therefore, we found that OPC can inhibit apoptosis by protecting mitochondria. Similar results have been proven in other kinds of cells (39)(40)(41)(42)(43). In this study, we used chondrocytes derived from mice and did not compare with chondrocytes of other animals.…”

Section: Discussionsupporting

confidence: 74%

Oligomeric proanthocyanidins inhibit apoptosis of chondrocytes induced by interleukin-1β

Yin

Wang

et al. 2017

Molecular Medicine Reports

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Oligomeric proanthocyanidin (OPC) is a water-soluble plant polyphenolic compound known for its cytoprotective effects in various tissue types. However, its effect on chondrocytes has not been well characterized. The present study aimed to investigate the effect of OPC on interleukin-1β (IL-1β)-induced apoptosis in chondrocytes, and to determine the mechanisms underlying the protective effects of OPC. Knee articular chondrocytes obtained from 6-week-old SPF Kunming mice were cultured and serially passaged. First-generation chondrocytes were selected for subsequent experiments following toluidine blue staining. Subsequent to IL-1β and OPC administration, an MTT assay was performed to examine the viability rate of chondrocytes, and the optimal drug concentration was determined. The fluorescence dye 2',7'-dichlorofluorescein diacetate was used to determine the intracellular content of reactive oxygen species (ROS). Mitochondrial membrane potential (MMP) was measured using a 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolylcarbocyanine iodide (JC-1) assay. The apoptosis rate of chondrocytes was assessed using an Annexin V-FITC/PI assay and ultrastructural changes were observed under an electron microscope. The results demonstrated that OPC increased the survival rate of chondrocytes against IL-1β-induced apoptosis. The most significant protective effect of OPC was observed at the concentration of 0.050 mg/ml. OPC reversed the increased ROS content and MMP levels, and inhibited IL-1β-induced apoptosis in chondrocytes. In addition, OPC was revealed to protect the ultrastructural integrity of chondrocytes. Taken together, the results of the present study suggest that OPC protects chondrocytes against IL-1β-induced damage by decreasing ROS content and MMP levels.

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Section: Discussionsupporting

confidence: 74%

Oligomeric proanthocyanidins inhibit apoptosis of chondrocytes induced by interleukin-1β

Yin

Wang

et al. 2017

Molecular Medicine Reports

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“…Intriguingly, our data further reveal that GSP cotreatment rescued the early apoptosis triggered by FB 1 . A study in lymphocytes reported that GSPE could reduce apoptosis induced by extracellular histones by upregulating BCL2 expression [56]. Collectively, our results indicate that the suppressive effects of GSP on apoptosis might be another mechanism against FB 1 toxicity in oocytes.…”

Section: Discussionsupporting

confidence: 54%

Grape Seed Proanthocyanidin Ameliorates FB1-Induced Meiotic Defects in Porcine Oocytes

Zhao

et al. 2021

Toxins

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Fumonisin B1 (FB1), as the most prevalent and toxic fumonisin, poses a health threat to humans and animals. The cytotoxicity of FB1 is closely related to oxidative stress and apoptosis. The purpose of this study is to explore whether Grape seed proanthocyanidin (GSP), a natural antioxidant, could alleviate the meiotic maturation defects of oocytes caused by FB1 exposure. Porcine cumulus oocyte complexes (COCs) were treated with 30 μM FB1 alone or cotreated with 100, 200 and 300 μM GSP during in vitro maturation for 44 h. The results show that 200 μM GSP cotreatment observably ameliorated the toxic effects of FB1 exposure, showing to be promoting first polar body extrusion and improving the subsequent cleavage rate and blastocyst development rate. Moreover, 200 μM GSP cotreatment restored cell cycle progression, reduced the proportion of aberrant spindles, improved actin distribution and protected mitochondrial function in FB1-exposed oocytes. Furthermore, reactive oxygen species (ROS) generation was significantly decreased and the mRNA levels of CAT, SOD2 and GSH-PX were obviously increased in the 200 μM GSP cotreatment group. Notably, the incidence of early apoptosis and autophagy level were also significantly decreased after GSP cotreatment and the mRNA expression levels of BAX, CASPASE3, LC3 and ATG5 were markedly decreased, whereas BCL2 and mTOR were observably increased in the oocytes after GSP cotreatment. Together, these results indicate that GSP could exert significant preventive effects on FB1-induced oocyte defects by ameliorating oxidative stress through repairing mitochondrial dysfunction.

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“…It has been confirmed that UTI can protect cells from apoptosis though anti-oxidation and reduction of mitochondrial damage. It is known that apoptosis contributes to immunoparalysis and death of sepsis patients (Hotchkiss and Nicholson, 2006; Hotchkiss et al, 2013; Chang et al, 2017b). Anti-apoptosis in sepsis models, via increase in Bcl-2 expression or blocking of CD95, reduced the incidence of sepsis-related mortality (Hotchkiss and Nicholson, 2006; Zhang et al, 2010; Sun et al, 2011; Liu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Improvement of Sepsis Prognosis by Ulinastatin: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

et al. 2019

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Background: Ulinastatin has been prescribed to treat sepsis. However, there is doubt regarding the extent of any improvement in outcomes to guide future decision making.Objectives: To evaluate the effects of ulinastatin on mortality and related outcomes in sepsis patients.Methods: Thirteen randomized controlled trials and two prospective studies published before September 1, 2018, that included 1358 patients with sepsis, severe sepsis, or septic shock were evaluated. The electronic databases searched in this study were PubMed, Medline, Embase, and China National Knowledge Infrastructure (CNKI) for Chinese Technical Periodicals.Results: Ulinastatin significantly decreased the all-cause mortality {odds ratio (OR) = 0.48, 95% confidence interval (CI) [0.35–0.66], p < 0.00001, I2 = 13%}, Acute Physiology, Age, Chronic Health Evaluation II (APACHE II) score {mean difference (MD) = −2.40, 95% CI [−4.37, −0.44], p = 0.02, I2 = 66%}, and reduced the incidence of multiple organ dysfunction syndrome (MODS) (OR = 0.3, 95% CI [0.18, 0.49], p < 0.00001, I2 = 0%). Ulinastatin also decreased the serum levels of IL-6 (MD = −88.5, 95% CI [−123.97, −53.04], p < 0.00001), TNF-α (MD = −56.22, 95% CI [−72.11, −40.33], p < 0.00001), and increased the serum levels of IL-10 (MD = 37.73, 95% CI [16.92, 58.54], p = 0.0004). Ulinastatin administration did not lead to any difference in the occurrence of adverse events.Conclusions: Ulinastatin improved all-cause mortality and other related outcomes in patients with sepsis or septic shock. The results of this meta-analysis suggest that ulinastatin may be an effective treatment for sepsis and septic shock.

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Grape seed proanthocyanidin extract protects lymphocytes against histone-induced apoptosis

Cited by 7 publications

References 44 publications

Oligomeric proanthocyanidins inhibit apoptosis of chondrocytes induced by interleukin-1β

Oligomeric proanthocyanidins inhibit apoptosis of chondrocytes induced by interleukin-1β

Grape Seed Proanthocyanidin Ameliorates FB1-Induced Meiotic Defects in Porcine Oocytes

Improvement of Sepsis Prognosis by Ulinastatin: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

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