Grapefruit juice and its furocoumarins inhibits autoinducer signaling and biofilm formation in bacteria

Girennavar, Basavaraj; Cepeda, Martha; Soni, Kamlesh A.; Vikram, Amit; Jesudhasan, Palmy R.; Jayaprakasha, G.K.; Pillai, Suresh D.; Patil, Bhimanagouda S.

doi:10.1016/j.ijfoodmicro.2008.03.028

Cited by 181 publications

(112 citation statements)

References 42 publications

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“…Extracts of fruits (e.g., blackberries, cranberries, vanilla, and citrus) and herbs (e.g., rosemary and turmeric) and extracts and oils of other plant materials (garlic, clove, and cinnamon) and medicinal plants (e.g., betel nut and notoginsing) have all displayed QSI activity to some degree, typically found via inhibition of violacein production in the C. violaceum bioassay (137,138,(264)(265)(266)(267)(268)(269)(270)(271)(272)(273)(274)(275). In very few cases, however, have individual compounds been isolated or identified from these kinds of complex sources.…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

690

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

690

556

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“…Also, the protective effect offered by adding pyrogallol to the brine shrimp culture water could be nullified by adding catalase but not by adding heat-inactivated catalase, again confirming that the effect of pyrogallol could be attributed to peroxide production resulting from the autooxidation of the compound. During the past decade, multiple reports have been published describing compounds as quorum-sensing inhibitors at so-called subinhibitory concentrations (13)(14)(15)(16)(17)(18)(19)(20). Our results emphasize that it is essential to verify the effect on viability by using sensitive methods (e.g., under nonoptimal growth conditions) and to include appropriate controls (e.g., tests on the same phenotype, but in a strain that is engineered in such a way to express the phenotype independently of quorum sensing) in order to confirm that such compounds really do interfere with quorum sensing.…”

mentioning

confidence: 99%

The Apparent Quorum-Sensing Inhibitory Activity of Pyrogallol Is a Side Effect of Peroxide Production

Defoirdt

Julyantoro

Baruah

et al. 2013

Antimicrob Agents Chemother

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There currently is more and more interest in the use of natural products, such as tea polyphenols, as therapeutic agents. The polyphenol compound pyrogallol has been reported before to inhibit quorum-sensing-regulated bioluminescence in Vibrio harveyi. Here, we report that the addition of 10 mg · liter ؊1 pyrogallol protects both brine shrimp (Artemia franciscana) and giant river prawn (Macrobrachium rosenbergii) larvae from pathogenic Vibrio harveyi, whereas the compound showed relatively low toxicity (therapeutic index of 10). We further demonstrate that the apparent quorum-sensing-disrupting activity is a side effect of the peroxide-producing activity of this compound rather than true quorum-sensing inhibition. Our results emphasize that verification of minor toxic effects by using sensitive methods and the use of appropriate controls are essential when characterizing compounds as being able to disrupt quorum sensing. P olyphenols are a large group of compounds found in plants, including coffee and tea (1). They have been reported to have both antioxidant (2) and prooxidant activities (3). The polyphenol compound pyrogallol (1,2,3-trihydroxybenzene) has been reported to have antibacterial activity against many bacteria, including vibrios (4). More recently, the compound has been reported to inhibit quorum-sensing-regulated bioluminescence at subinhibitory concentrations (i.e., concentrations that did not affect growth) in a Vibrio harveyi HAI-1 receptor mutant (50% inhibitory concentration [IC 50 ] of 2 M [ϳ0.25 mg · liter Ϫ1 ]) (5). However, the effect of pyrogallol on the bioluminescence of a constitutively luminescent strain (which would allow verification that the bioluminescence inhibition is really caused by interference with its regulation) has not been studied, nor has the compound been reported to affect any other quorum-sensing-regulated phenotype in the bacterium.Impact of pyrogallol on the virulence of Vibrio harveyi toward brine shrimp larvae. We previously showed that the virulence of Vibrio harveyi BB120 (ϭ ATCC BAA-1116; recently reclassified as Vibrio campbellii [6]) in our model system with gnotobiotic brine shrimp larvae is regulated by quorum sensing (7). Using this model system, we investigated whether pyrogallol could protect challenged larvae from the pathogen and found that when added to the culture water at 10 mg · liter Ϫ1 or more, the compound significantly increased the survival of challenged larvae (Table 1). Furthermore, pyrogallol showed relatively low toxicity to brine shrimp larvae, as there was no significant negative effect on the survival of nonchallenged larvae for concentrations up to 100 mg · liter Ϫ1 (Table 1). Impact of pyrogallol on the virulence of Vibrio harveyi toward giant river prawn larvae. Because of these promising results, we went further to investigate the effect of pyrogallol in a commercial crustacean species. We had previously found that the virulence of Vibrio harveyi to larvae of the giant river prawn Macrobrachium rosenbergii is regulated by quorum se...

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“…However, synthetic furonone derivatives showed significant anti-QS activity in P. aeruginosa (2,25). Natural or synthetic furanone compounds were also found to inhibit a range of QS regulated phenotypes in a variety of bacterial species including E. coli O157:H7 and Salmonella enterica serovar Typhimurium and Serratia liquefaciens (26,27). These results suggest that furanone compounds could serve as a source to develop furanone-based drugs to block quorum-sensing systems in many Gram (-) bacteria.…”

Section: Qs Inhibitorsmentioning

confidence: 93%

Disruption of Bacterial cell-to-cell communication (Quorum Sensing): A Promising Novel Way to Combat Bacteria-Mediated Diseases

Tinaz¹

2013

MUSBED

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Grapefruit juice and its furocoumarins inhibits autoinducer signaling and biofilm formation in bacteria

Cited by 181 publications

References 42 publications

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

The Apparent Quorum-Sensing Inhibitory Activity of Pyrogallol Is a Side Effect of Peroxide Production

Disruption of Bacterial cell-to-cell communication (Quorum Sensing): A Promising Novel Way to Combat Bacteria-Mediated Diseases

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