Grapevine Vp<scp>PR</scp>10.1 functions in resistance to <i>Plasmopara viticola</i> through triggering a cell death‐like defence response by interacting with Vp<scp>VDAC</scp>3

Ma, Hui; Xiang, Gaoqing; Li, Zhiqian; Wang, Yuting; Dou, Mengru; Li, Su; Yin, Xiao; Liu, Ruiqi; Wang, Yuejin

doi:10.1111/pbi.12891

Cited by 48 publications

(30 citation statements)

References 104 publications

(139 reference statements)

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“…The full-length sequence of AtHY5 ( ELONGATED HYPOCOTYL 5 ; AT5G11260) was cloned into the pBI221-mCherry vector to generate AtHY5-mCherry. Sequence-verified constructions were introduced into N. benthamiana protoplasts using a PEG-calcium transfection method (Yoo et al, 2007; Ma et al, 2018). The transformed N. benthamiana protoplasts or agroinfiltrated leaves were observed using confocal microscopy (TCS SP8, Leica, Germany).…”

Section: Methodsmentioning

confidence: 99%

The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara viticola Induces Cell Death and Immunity Responses in Nicotiana benthamiana

et al. 2019

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Downy mildew is one of the most serious diseases of grapevine ( Vitis spp). The causal agent of grapevine downy mildew, Plasmopara viticola , is an obligate biotrophic oomycete. Although oomycete pathogens such as P. viticola are known to secrete RxLR effectors to manipulate host immunity, there have been few studies of the associated mechanisms by which these may act. Here, we show that a candidate P. viticola RxLR effector, PvAvh74, induces cell death in Nicotiana benthamiana leaves. Using agroinfiltration, we found that nuclear localization, two putative N -glycosylation sites, and 427 amino acids of the PvAvh74 carboxyl terminus were necessary for cell-death-inducing activity. Using virus-induced gene silencing (VIGS), we found that PvAvh74-induced cell death in N. benthamiana requires EDS1, NDR1, SGT1, RAR1, and HSP90, but not BAK1. The MAPK cascade components MEK2, WIPK, and SIPK were also involved in PvAvh74-induced cell death in N. benthamiana . Transient expression of PvAvh74 could suppress Phytophthora capsici colonization of N. benthamiana , which suggests that PvAvh74 elicits plant immune responses. Suppression of P. capsici colonization also was dependent on nuclear localization of PvAvh74. Additionally, PvAvh74-triggered cell death could be suppressed by another effector, PvAvh8, from the same isolate. This work provides a framework to further investigate the interactions of PvAvh74 and other RxLR effectors with host immunity.

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Section: Methodsmentioning

confidence: 99%

The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara viticola Induces Cell Death and Immunity Responses in Nicotiana benthamiana

et al. 2019

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“…Some of these enzymes (functional annotations) facilitate backbone deconstruction (e.g., endo-polygalacturonase, pectin lyase, pectin methyl-esterase), and side chain cleavage (e.g., arabinase) [32]. The infected grapes detect the pathogen and mount a defense response [33,34]. Oligosaccharides released from HG backbone of the grape cell wall can act as potent defense response elicitors and activate plant immune responses [35,36,37].…”

Section: Grape Cell Wall Integrity Associated With Ripening and Bementioning

confidence: 99%

Deconstructing Wine Grape Cell Walls with Enzymes During Winemaking: New Insights from Glycan Microarray Technology

et al. 2019

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Enzyme-aid maceration is carried out in most modern winemaking industries with a range of positive impacts on wine production. However, inconsistencies in enzyme efficiency are an issue complicated by unclear targets (limited information available on berry cell wall architecture of different cultivars) and the complex wine environment (i.e., fermenting must). Recent studies have been performed to develop a clearer picture of grape cell wall structures, maceration effects, and interactions between important wine compounds and grape-derived polysaccharides. This review highlights critically important recent studies on grape berry cell wall changes during ripening, the importance of enzymes during maceration (skin contact phase) and deconstruction processes that occur during alcoholic fermentation. The novelty of the Comprehensive Microarray Polymer Profiling (CoMPP) technique using cell wall probes (e.g., antibodies) as a method for following cell wall derived polymers during different biological and biotechnological processes is discussed. Recent studies, using CoMPP together with classical analytical methods, confirmed the developmental pattern of berry cell wall changes (at the polymer level) during grape ripening. This innovative technique were also used to track enzyme-assisted depectination of grape skins during wine fermentation and determine how this influence the release of wine favourable compounds. Furthermore, polysaccharides (e.g., arabinogalactan proteins) present in the final wine could be identified. Overall, CoMPP provides a much more enriched series of datasets compared to traditional approaches. Novel insights and future studies investigating grape cell wall and polyphenol interactions, and the tailoring of enzyme cocktails for consistent, effective and “customized” winemaking is advanced and discussed.

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“…Recent several studies have revealed that ROS are essential for maintaining normal cellular functions, as opposed to having a toxic effect on cells [2]. Even cell death that was previously considered a result from oxidative damage is now thought to be a ROS-mediated physiological pathway for programmed cell death, rather than directly killing the cells [3,4]. Additionally, ROS levels that are too low are cytostatic for cells, whereas ROS levels that are too high are cytotoxic [5].…”

Section: Introductionmentioning

confidence: 99%

The Catalase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis

Wang

Cheng

Chen

et al. 2019

Cells

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Catalases (CATs), which were coded by the catalase gene family, were a type notably distinguished ROS-metabolizing proteins implicated to perform various physiological functions in plant growth, development and stress responses. However, no systematical study has been performed in cotton. In the present study, we identified 7 and 7 CAT genes in the genome of Gossypium hirsutum L. Additionally, G. barbadense L., respectively. The results of the phylogenetic and synteny analysis showed that the CAT genes were divided into two groups, and whole-genome duplication (WGD) or polyploidy events contributed to the expansion of the Gossypium CAT gene family. Expression patterns analysis showed that the CAT gene family possessed temporal and spatial specificity and was induced by the Verticillium dahliae infection. In addition, we predicted the putative molecular regulatory mechanisms of the CAT gene family. Based on the analysis and preliminary verification results, we hypothesized that the CAT gene family, which might be regulated by transcription factors (TFs), alternative splicing (AS) events and miRNAs at different levels, played roles in cotton development and stress tolerance through modulating the reactive oxygen species (ROS) metabolism. This is the first report on the genome-scale analysis of the cotton CAT gene family, and these data will help further study the roles of CAT genes during stress responses, leading to crop improvement.

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Grapevine VpPR10.1 functions in resistance to Plasmopara viticola through triggering a cell death‐like defence response by interacting with VpVDAC3

Cited by 48 publications

References 104 publications

The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara viticola Induces Cell Death and Immunity Responses in Nicotiana benthamiana

The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara viticola Induces Cell Death and Immunity Responses in Nicotiana benthamiana

Deconstructing Wine Grape Cell Walls with Enzymes During Winemaking: New Insights from Glycan Microarray Technology

The Catalase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis

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