Graphene: An Antibacterial Agent or a Promoter of Bacterial Proliferation?

Zhang, Tian; Tremblay, Pier-Luc

doi:10.1016/j.isci.2020.101787

Cited by 56 publications

(39 citation statements)

References 292 publications

(242 reference statements)

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“…Furthermore, a stimulative potential of nanomaterials was revealed in this test, particularly with the flake-like nanomaterials (i.e., purified CoOF and GO). At the end of the experiment, these structures reached the highest OD, which agrees with the literature, where a growth stimulation by graphene was described (Zhang and Tremblay 2020). Another nanomaterial containing titanium dioxide (CNT TiO 2 ) did not delay the exponential growth, although it significantly slowed down MGR.…”

Section: Growth Curvessupporting

confidence: 90%

“…This effect was powerful after 24 h. In the current literature, one can find evidence of the toxicity of graphene oxide against microorganisms and its growth enhancement potential, with the first option as the Fig. 1 The optical density of cultures after co-incubation with nanomaterials for 6, 12, and 24 h; *sample statistically different from the control sample (0) with p < 0.05 most frequent description (Yousefi et al 2017;Zhang and Tremblay 2020). Considering also other "flake-like" structures considered in this study, it appears that these structures were rather biocompatible in the in vitro conditions created in our experiments.…”

Section: Optical Density and Fluorescencementioning

confidence: 97%

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Basic physiology of Pseudomonas aeruginosa contacted with carbon nanocomposites

et al. 2022

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Experiments describing properties of nanomaterials on bacteria are frequently limited to the disk diffusion method or other end-point methods indicating viability or survival rate in plate count assay. Such experimental design does not show the dynamic changes in bacterial physiology, mainly when performed on reference microorganisms (Escherichia coli and Staphylococcus aureus). Testing other microorganisms, such as Pseudomonas aeruginosa, could provide novel insights into the microbial response to nanomaterials. Therefore, we aimed to test selected carbon nanomaterials and their components in a series of experiments describing the basic physiology of P. aeruginosa. Concentrations ranging from 15.625 to 1000 µg/mL were tested. The optical density of cultures, pigment production, respiration, growth curve analysis, and biofilming were tested. The results confirmed variability in the response of P. aeruginosa to tested nanostructures, depending on their concentration. The co-incubation with the nanostructures (in concentration 125 µg/mL) could inhibit the population growth (in most cases) or promote it in the case of graphene oxide. Furthermore, a specific concentration of a given nanomaterial could cause contradictory effects leading to stimulation or inhibition of pigmentation, an optical density of the cultures, or biofilm formation. We have found that particularly nanomaterials containing TiO2 could induce pigmentation in P. aeruginosa, which indicates the possibility of increased virulence. On the other hand, nanocomposites containing cobalt nanoparticles had the highest anti-bacterial potential when cobalt was displayed on the surface. Our approach revealed changes in respiration and growth dynamics that can be used to search for nanomaterials’ application in biotechnology.

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Section: Growth Curvessupporting

confidence: 90%

Section: Optical Density and Fluorescencementioning

confidence: 97%

Basic physiology of Pseudomonas aeruginosa contacted with carbon nanocomposites

et al. 2022

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“…Three main types of graphene materials: graphene (Gr), graphene oxide (GO), and reduced graphene oxide (RGO) are in use for the development of new antimicrobial agents. Their antimicrobial activity depends significantly on the method of synthesis, which determines the sheet number and thickness, as well as the oxygen content [ 26 , 27 ].…”

Section: Antimicrobial Activity and Applications Of Graphene Nanomaterialsmentioning

confidence: 99%

“…This raised the question of whether graphene is an antibacterial agent or a promoter of cell proliferation. To answer this, Zhang et al [ 27 ] debated the mechanisms and factors determining the positive or negative impact of Gr materials on bacteria and summarized that adjustable physicochemical properties and environmental factors determine whether the Gr materials will act as antibacterial materials or will promote bacterial growth. The toxicity of Gr materials toward bacteria is partly explained by their capacity to cause oxidative stress by ROS generated from molecular oxygen.…”

Section: Proposed Mechanisms Of Microbial Adhesion Inhibition By Graphene-based Nanomaterialsmentioning

confidence: 99%

Antibiofouling Activity of Graphene Materials and Graphene-Based Antimicrobial Coatings

et al. 2021

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Microbial adhesion and biofilm formation is a common, nondesirable phenomenon at any living or nonliving material surface in contact with microbial species. Despite the enormous efforts made so far, the protection of material surfaces against microbial adhesion and biofilm formation remains a significant challenge. Deposition of antimicrobial coatings is one approach to mitigate the problem. Examples of such are those based on heparin, cationic polymers, antimicrobial peptides, drug-delivering systems, and other coatings, each one with its advantages and shortcomings. The increasing microbial resistance to the conventional antimicrobial treatments leads to an increasing necessity for new antimicrobial agents, among which is a variety of carbon nanomaterials. The current review paper presents the last 5 years’ progress in the development of graphene antimicrobial materials and graphene-based antimicrobial coatings that are among the most studied. Brief information about the significance of the biofouling, as well as the general mode of development and composition of microbial biofilms, are included. Preparation, antibacterial activity, and bactericidal mechanisms of new graphene materials, deposition techniques, characterization, and parameters influencing the biological activity of graphene-based coatings are focused upon. It is expected that this review will raise some ideas for perfecting the composition, structure, antimicrobial activity, and deposition techniques of graphene materials and coatings in order to provide better antimicrobial protection of medical devices.

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“…By the functionalization of CS (positively charged) with CA, the CsA molecules became negatively charged due to the COO − groups originated from CA. In the presence of Gram (−) P. aeruginosa, the electrostatic repulsions occur between the negatively charged cell surface and CsA with negative charges, leading to the growth inhibition of the Gram (−) bacterial strain on the surface of fibrous membranes [56]. In addition, the literature reports that the bacteria with elongated shape (Gram −) are more disturbed compared to the ones with spherical shape (Gram +) due to the larger contact surface area [57].…”

Section: Anti-biofilm Activity Of Nanofibrous Membranesmentioning

confidence: 99%

Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings

et al. 2022

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The present research work is focused on the design and investigation of electrospun composite membranes based on citric acid-functionalized chitosan (CsA) containing reduced graphene oxide-tetraethylene pentamine (CsA/rGO-TEPA) as materials with opportune bio-properties for applications in wound dressings. The covalent functionalization of chitosan (CS) with citric acid (CA) was achieved through the EDC/NHS coupling system and was checked by 1H-NMR spectroscopy and FTIR spectrometry. The mixtures to be electrospun were formulated by adding three concentrations of rGO-TEPA into the 1/1 (w/w) CsA/poly (ethylene oxide) (PEO) solution. The effect of rGO-TEPA concentration on the morphology, wettability, thermal stability, cytocompatibility, cytotoxicity, and anti-biofilm activity of the nanofibrous membranes was extensively investigated. FTIR and Raman results confirmed the covalent and non-covalent interactions that appeared between the system’s compounds, and the exfoliation of rGO-TEPA sheets within the CsA in the presence of PEO (CsA/P) polymer matrix, respectively. SEM analysis emphasized the nanofibrous architecture of membranes and the presence of rGO-TEPA sheets entrapped into the CsA nanofiber structure. The MTT cellular viability assay showed a good cytocompatibility with the highest level of cell development and proliferation registered for the CsA/P composite nanofibrous membrane with 0.250 wt.% rGO-TEPA. The designed nanofibrous membranes could have potential applications in wound dressings, given that they showed a good anti-biofilm activity against Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacterial strains.

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Graphene: An Antibacterial Agent or a Promoter of Bacterial Proliferation?

Cited by 56 publications

References 292 publications

Basic physiology of Pseudomonas aeruginosa contacted with carbon nanocomposites

Basic physiology of Pseudomonas aeruginosa contacted with carbon nanocomposites

Antibiofouling Activity of Graphene Materials and Graphene-Based Antimicrobial Coatings

Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings

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