Graphene-based conductive fibrous scaffold boosts sciatic nerve regeneration and functional recovery upon electrical stimulation

Dong, Chanjuan; Qiao, Fangyu; Hou, Wensheng; Yang, Li; Lv, Yonggang

doi:10.1016/j.apmt.2020.100870

Cited by 34 publications

(38 citation statements)

References 59 publications

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“…For instance, applying ES with an intensity of 50 mv/cm for one h/day through sodium dodecyl benzenesulfonate (DBS)/GO/polypyrrole-poly-l-lactic acid (PLLA) nanofiber can significantly promote neurite elongation and alignment [ 53 ]. Another study represents that significant intensity of ES may damage the neural cells and tissue, thus fabricating electrical responsive scaffolds that can deliver electrical signals to neuron cells and modulate cellular behavior for developing functional connection is highly recommended [ 32 , 52 , 54 ]. Applying a nominal ES intensity of 10 mv (1 h/day) alongside a superior conductive scaffold (4% G/thermoplastic polyurethane) with conductivity of 33.45 ± 0.78 S/m would be an ideal candidate for guiding neural cell growth [ 32 ].…”

Section: Tissue-engineered Scaffolds With Gbm and Neural Tissuesmentioning

confidence: 99%

Graphene-Based Materials Prove to Be a Promising Candidate for Nerve Regeneration Following Peripheral Nerve Injury

et al. 2021

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Peripheral nerve injury is a common medical condition that has a great impact on patient quality of life. Currently, surgical management is considered to be a gold standard first-line treatment; however, is often not successful and requires further surgical procedures. Commercially available FDA- and CE- approved decellularized nerve conduits offer considerable benefits to patients suffering from a completely transected nerve but they fail to support neural regeneration in gaps >30 mm. To address this unmet clinical need, current research is focused on biomaterial-based therapies to regenerate dysfunctional neural tissues, specifically damaged peripheral nerve, and spinal cord. Recently, attention has been paid to the capability of graphene-based materials (GBMs) to develop bifunctional scaffolds for promoting nerve regeneration, often via supporting enhanced neural differentiation. The unique features of GBMs have been applied to fabricate an electroactive conductive surface in order to direct stem cells and improve neural proliferation and differentiation. The use of GBMs for nerve tissue engineering (NTE) is considered an emerging technology bringing hope to peripheral nerve injury repair, with some products already in preclinical stages. This review assesses the last six years of research in the field of GBMs application in NTE, focusing on the fabrication and effects of GBMs for neurogenesis in various scaffold forms, including electrospun fibres, films, hydrogels, foams, 3D printing, and bioprinting.

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Section: Tissue-engineered Scaffolds With Gbm and Neural Tissuesmentioning

confidence: 99%

Graphene-Based Materials Prove to Be a Promising Candidate for Nerve Regeneration Following Peripheral Nerve Injury

et al. 2021

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“…Therefore, appropriate electrical stimulation for cells encapsulated in the scaffolds and host neurons and glia is speculated to promote axon survival and migration [ 220 , 221 ]. Dong et al incorporated graphene into electrospun fibrous scaffolds, and they found that electrical stimulation could accelerate cell migration and promote neurotrophic factor secretion in vitro as well as enhance sciatic nerve regeneration and functional recovery after implantation [ 222 ]. By including conductive materials, like gold nanoparticles, graphene, polyaniline, etc., we can combine an anisotropic topography with electrical stimulation to study their synergistic effects on nerve regeneration.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Xue

Shi

Kong

et al. 2021

Bioactive Materials

112

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The treatment of long-gap (>10 mm) peripheral nerve injury (PNI) and spinal cord injury (SCI) remains a continuous challenge due to limited native tissue regeneration capabilities. The current clinical strategy of using autografts for PNI suffers from a source shortage, while the pharmacological treatment for SCI presents dissatisfactory results. Tissue engineering, as an alternative, is a promising approach for regenerating peripheral nerves and spinal cords. Through providing a beneficial environment, a scaffold is the primary element in tissue engineering. In particular, scaffolds with anisotropic structures resembling the native extracellular matrix (ECM) can effectively guide neural outgrowth and reconnection. In this review, the anatomy of peripheral nerves and spinal cords, as well as current clinical treatments for PNI and SCI, is first summarized. An overview of the critical components in peripheral nerve and spinal cord tissue engineering and the current status of regeneration approaches are also discussed. Recent advances in the fabrication of anisotropic surface patterns, aligned fibrous substrates, and 3D hydrogel scaffolds, as well as their in vitro and in vivo effects are highlighted. Finally, we summarize potential mechanisms underlying the anisotropic architectures in orienting axonal and glial cell growth, along with their challenges and prospects.

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“…In this context, the crosslinking procedure applied to the nerve conduit can be critically relevant because it strongly affects its mechanical properties and stiffness and, consequently, the macrophage response [180]. Some of the chemical crosslinking agents commonly used are formaldehyde, hexamethylene diisocyanate, glutaraldehyde (GA), polyepoxy compounds, carbodiimides (EDAC, EDC) and genipin [180,188,189]. The effects induced by the crosslinking procedure on macrophages phenotype are described in a 2019 study by Kočí et al Here, they compared the effects of genipin and formaldehyde on collagen-based nerve guidance conduits.…”

Section: Chemical Modificationsmentioning

confidence: 99%

“…Dong et al demonstrated that the association of graphene-based conductive fibrous scaffold and exogenous electrical stimulation is an efficient method to improve nerve regeneration and direct macrophage polarization. Specifically, it was assessed that the graphene-based conduits, when crossed by an external current, had a significantly increased number of macrophages positive for CD163, a marker of the anti-inflammatory phenotype, in comparison to the other scaffolds analyzed [189]. In a similar study by Agarwal et al, a graphene crosslinked collagen-based nerve conduit, when seeded with raw 264.7 macrophages in vitro, induced a high expression of anti-inflammatory markers CD163 and CD206.…”

Section: Microporous Nanodiamonds/pcl Nerve Bridgementioning

confidence: 99%

“…Finally, self-assembling peptides (SAPs) comprise a family of biocompatible, biodegradable and easily modifiable short chain amino acid sequences, [317], capable of forming hydrogels, and have shown considerable promise for CNS injury applications [318]. SAPs have demonstrated good integration into the lesioned spinal cord and promote axonal growth [188,189] and do not induce an immune response with CD68+ macrophage numbers similar to lesioned cord controls as long as 4 weeks after injury [319,320]. Because subtyping into M1 and M2 macrophages was not explored, it is not possible to determine if SAPs may exhibit immunomodulatory functions.…”

Section: Chemical Modificationsmentioning

confidence: 99%

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Biomaterial and Therapeutic Approaches for the Manipulation of Macrophage Phenotype in Peripheral and Central Nerve Repair

et al. 2021

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Injury to the peripheral or central nervous systems often results in extensive loss of motor and sensory function that can greatly diminish quality of life. In both cases, macrophage infiltration into the injury site plays an integral role in the host tissue inflammatory response. In particular, the temporally related transition of macrophage phenotype between the M1/M2 inflammatory/repair states is critical for successful tissue repair. In recent years, biomaterial implants have emerged as a novel approach to bridge lesion sites and provide a growth-inductive environment for regenerating axons. This has more recently seen these two areas of research increasingly intersecting in the creation of ‘immune-modulatory’ biomaterials. These synthetic or naturally derived materials are fabricated to drive macrophages towards a pro-repair phenotype. This review considers the macrophage-mediated inflammatory events that occur following nervous tissue injury and outlines the latest developments in biomaterial-based strategies to influence macrophage phenotype and enhance repair.

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Graphene-based conductive fibrous scaffold boosts sciatic nerve regeneration and functional recovery upon electrical stimulation

Cited by 34 publications

References 59 publications

Graphene-Based Materials Prove to Be a Promising Candidate for Nerve Regeneration Following Peripheral Nerve Injury

Graphene-Based Materials Prove to Be a Promising Candidate for Nerve Regeneration Following Peripheral Nerve Injury

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Biomaterial and Therapeutic Approaches for the Manipulation of Macrophage Phenotype in Peripheral and Central Nerve Repair

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