Graphene-based fluorometric determination of agrD gene transcription in methicillin-resistant Staphylococcus aureus using exonuclease III-aided target recycling and DNA walker cascade amplification

Ning, Yi; Wang, Xiaoqi; Hu, Jue; Li, Ling; Xiao, Rong; Lu, Fangguo

doi:10.1007/s00604-021-04933-8

Cited by 7 publications

(1 citation statement)

References 35 publications

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“…More importantly, compared with some other reported methods, the proposed sensor has a clearer design principle, lower cost, and simpler operation. [42][43][44] 3.5 | Repeatability, stability, and specificity of biosensor…”

Section: Electrochemical Detection Of Target Dnamentioning

confidence: 99%

A sensitive electrochemical DNA sensor based on reduced graphene oxide modified electrode

Shu

et al. 2022

J Chinese Chemical Soc

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In this work, an electrochemical sensing platform, analogous to a DNA walker, was explored with exonuclease III (EXO III) as a driving force, tetraferrocene as a signal marker, and reduced graphene oxide (RGO) electrode as twodimensional (2D) orbit to realize ultrasensitive detection of target DNA. We designed a single hairpin DNA probe with 3 0 -end modified tetraferrocene, immobilized on the electrode surface by π-π stacking between the 5 0 terminus and reduced graphene oxide. In addition, EXO III digested the double-stranded DNA with a blunt 3 0 terminus formed by the target DNA and the probe DNA, thus degrading the 3 0 end of probe DNA, and resulting in the away movement of tetraferrocene from the electrode surface, decreasing the electrochemical signal. The proposed electrochemical sensor was very simple and stable with acceptable reproducibility, excellent DNA detection performance, and a detection limit as low as 0.3 pM.

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Section: Electrochemical Detection Of Target Dnamentioning

confidence: 99%