Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7

Pandey, Ashish; Gürbüz, Yaşar; Ozguz, V.; Niazi, Javed H.; Qureshi, Anjum

doi:10.1016/j.bios.2016.12.041

Cited by 146 publications

(64 citation statements)

References 33 publications

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“…An electrochemical biosensor for its detection was constructed by interfacing graphene nanostructures functionalized with specific antibodies able to immobilize bacteria on the sensor surface. The developed device provided non-faradaic electrochemical responses related to the number of cells per mL, with no need for redox probe, and allowed the detection of bacteria to as low as 10-100 cells mL −1 [114].…”

Section: Microorganismsmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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Section: Microorganismsmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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“…Additionally, graphene shows excellent thermal and electrical conductivity, lower charge‐transfer resistance and broad electrochemical potential which lead to amplification of electrical pathogenic detection signal, lower detection (higher sensitivity) . Thus, graphene can well perform in direct redox electron transfer between active microorganisms and working electrode . Finally, graphene production process itself is ease, safe and cost‐effective (mainly from graphene oxide reduction) which will lower the cost of microbial sensor .…”

Section: Basic Concepts Of Bioelectrochemistrymentioning

confidence: 99%

Bioelectrochemical Systems for Measuring Microbial Cellular Functions

Selim

Kamal²,

Ali

et al. 2017

Electroanalysis

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In bioelectrochemical systems (BESs), living microorganisms are capable of converting the chemical energy of degradable organic matters into bioelectricity. The electrical current outputs are dependent on the microbial cell viability and the biodegradation rates. Therefore, monitoring the current generative through the BES is promising for the microbial activity assessments. As compared to conventional microbiological methods, BESs are considered as non‐invasive techniques that offer rapid and sensitive detection of cellular functions (extra‐ and/or intracellular). Therefore, several progressions were made in the last 100 years in order to develop effective BESs. In this review, the involvements of materials sciences, microbiology, and electrochemistry in the effective designing and developments of BESs were intensively discussed. Due to the nanotechnology revolutions, manipulation of electrode materials led to the creation of different BES generations. Therefore, the impact of nanomaterials on the developments of the second and third generations of BESs is still the outlook of this promising research area.

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“…Several reports have shown the detection of Gram‐positive bacterial contamination in food and water based on a combination of different technologies and materials. Most of these detection methods rely on optical, electronic, and electrochemical techniques that are fast and easy to use . However, each of these methods has various disadvantages: optical methods generally use fluorescence or color development probes wherein any turbidity in the sample matrix affects sensitivity and detection limits; electrochemical methods are limited by the need of electroactive substrates in addition to background signals caused by various interfering ionic species .…”

Section: Introductionmentioning

confidence: 99%

MoS₂/TiO₂ Hybrid Nanostructure‐Based Field‐Effect Transistor for Highly Sensitive, Selective, and Rapid Detection of Gram‐Positive Bacteria

Moudgil

Singh

Mishra

et al. 2019

Adv Materials Technologies

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Field‐effect transistor (FET) biosensors based on hybrid nanostructures present unique advantages of high sensitivity, small size, excellent dynamic range, and compatibility with integrated circuits. In this work, the fabrication of highly sensitive and selective hybrid MoS2/TiO2 nanostructure‐based FET is demonstrated for the detection of Gram‐positive bacteria. The fabricated FET biosensors are efficiently capable of discriminating between Gram‐positive and Gram‐negative bacteria with a limit of detection 50 cfu mL−1. The biosensor exhibits a high sensitivity of 49.47 ± 1.7% for Staphylococcus aureus among eight different bacteria at a concentration of 102 cfu mL−1. The real time detection of bacteria with a response time of 22.19 s is achieved at concentrations as low as 50–100 cfu mL−1, suggesting rapid and low concentration detection of selective bacteria. The FET biosensor is also able to quantify bacteria in the physiological conditions as well as discriminate between live and dead bacteria. The unique hybrid nanostructure based FET with desirable biosensing properties presents the potential to screen the pathogenic bacteria. Live/dead monitoring of bacteria may help in deciding the course and dosage of the antibiotic treatment.

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Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7

Cited by 146 publications

References 33 publications

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

Bioelectrochemical Systems for Measuring Microbial Cellular Functions

MoS₂/TiO₂ Hybrid Nanostructure‐Based Field‐Effect Transistor for Highly Sensitive, Selective, and Rapid Detection of Gram‐Positive Bacteria

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Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7

Cited by 146 publications

References 33 publications

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

Bioelectrochemical Systems for Measuring Microbial Cellular Functions

MoS2/TiO2 Hybrid Nanostructure‐Based Field‐Effect Transistor for Highly Sensitive, Selective, and Rapid Detection of Gram‐Positive Bacteria

Contact Info

Product

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About

MoS₂/TiO₂ Hybrid Nanostructure‐Based Field‐Effect Transistor for Highly Sensitive, Selective, and Rapid Detection of Gram‐Positive Bacteria