Graphene-Orange II composite nanosheets with electroactive functions as label-free aptasensing platform for “signal-on” detection of protein

Guo, Yujing; Han, Yujie; Guo, Yanxiu; Dong, Chuan

doi:10.1016/j.bios.2013.01.054

Cited by 46 publications

(21 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensing principles applied rely on: displacement of LBA from its methylene blue-tagged DNA complex in the presence of lysozyme, resulting in a conformational change of methylene blue-tagged DNA into a hairpin structure; this brings methylene blue closer to the electrode surface, leading to an increase of its signal (signal-on sensor) [90] 4. displacement of LBA from its complex with DNA upon lysozyme addition (signal-off sensor) [ desorption of lysozyme aptamer from rGO/Orange II-modified GCE, reversing the blocking effect and reestablishing efficient electron transfer from graphene-adsorbed aromatic dye Orange II [85] Competitive detection schemes are typically used with regenerable sensors, where in order to revert to the characteristics of the original interface, the aptasensor needs simply to be re-incubated in fresh aptamer solution at the end of each measurement [62,89]. An ingenious detection strategy for multiple protein detection relied on immobilization of dabcyl-labeled-aptamer-modified metal nanoparticles (DLAPs) on a β-cyclodextrin-modified electrode by host-guest affinity [89].…”

Section: Electrochemical Assay Formats: Direct Sandwich and Competitmentioning

confidence: 99%

“…adsorption or π-π stacking interactions between the DNA bases of the lysozyme aptamer and graphene oxide-modified interfaces ( Figure 2A) [83][84][85] 2.…”

Section: Surface Immobilization Of Aptamer Ligandsmentioning

confidence: 99%

“…The hollow TiO2 spheres provided a large surface, porous support for 3D-graphene and polypyrrole, acting synergistically with these two materials to facilitate the immobilization of significant amounts of aptamer ( Figure 3B). For Case (1), polarization of carbon paste electrodes (CPE) at +0.5 V for 2 min [81] or modification via drop casting with reduced graphene oxide (rGO) [83], rGO/Fe 2 O 3 or rGO/Orange II nanocomposites [84,85] results in interfaces with the ability to strongly interact with aptamers via π-π stacking.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

View full text Add to dashboard Cite

Protein analysis and quantification are required daily by thousands of laboratories worldwide for activities ranging from protein characterization to clinical diagnostics. Multiple factors have to be considered when selecting the best detection and quantification assay, including the amount of protein available, its concentration, the presence of interfering molecules, as well as costs and rapidity. This is also the case for lysozyme, a 14.3-kDa protein ubiquitously present in many organisms, that has been identified with a variety of functions: antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods or a model of amyloid-type protein aggregation. Since the design of the first lysozyme aptamer in 2001, lysozyme became one of the most intensively-investigated biological target analytes for the design of novel biosensing concepts, particularly with regards to electrochemical aptasensors. In this review, we discuss the state of the art of aptamer-based electrochemical sensing of lysozyme, with emphasis on sensing in serum and real samples.

show abstract

Section: Electrochemical Assay Formats: Direct Sandwich and Competitmentioning

confidence: 99%

“…adsorption or π-π stacking interactions between the DNA bases of the lysozyme aptamer and graphene oxide-modified interfaces ( Figure 2A) [83][84][85] 2.…”

Section: Surface Immobilization Of Aptamer Ligandsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

View full text Add to dashboard Cite

show abstract

“…et al, 2013), and fluorescent aptasensor (Kong et al, 2013). Although some amplification-based or reagent-contained techniques show better detection limits down to picomolar levels (Cai et al, 2006;Guo et al, 2013;Hu et al, 2015;Jiang et al, 2013;Liu et al, 2014;Wang et al, 2013;Zhang et al, 2011), our technique may find an application with features on simplicity and highthroughput modality.…”

Section: Entrymentioning

confidence: 92%

Dual aptamer-immobilized surfaces for improved affinity through multiple target binding in potentiometric thrombin biosensing

Goda

Higashi

Matsumoto

et al. 2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

We developed a label-free and reagent-less potentiometric biosensor with improved affinity for thrombin. Two different oligomeric DNA aptamers that can recognize different epitopes in thrombin were introduced in parallel or serial manners on the sensing surface to capture the target via multiple contacts as found in many biological systems. The spacer and linker in the aptamer probes were optimized for exerting the best performance in molecular recognition. To gain the specificity of the sensor to the target, an antifouling molecule, sulfobeaine-3-undecanethiol (SB), was introduced on the sensor to form a self-assembled monolayer (SAM). Surface characterization revealed that the aptamer probe density was comparable to the distance of the two epitopes in thrombin, while the backfilling SB SAM was tightly aligned on the surface to resist nonspecific adsorption. The apparent binding parameters were obtained by thrombin sensing in potentiometry using the 1:1 Langmuir adsorption model, showing the improved dissociation constants (Kd) with the limit of detection of 5.5 nM on the dual aptamer-immobilized surfaces compared with single aptamer-immobilized ones. A fine control of spacer and linker length in the aptamer ligand was essential to realize the multivalent binding of thrombin on the sensor surface. The findings reported herein are effective for improving the sensitivity of potentiometric biosensor in an affordable way towards detection of tiny amount of biomolecules.

show abstract

“…Singal et al and Guo et al also reported sensitive protein detection using graphene-modified electrodes to amplify the detection signal. 27,28 While these sensors can achieve good analytical performance, they require complicated sensor modification procedures or lack the sensitivity required for detecting very low biomarker concentrations in raw biofluids.…”

Section: Introductionmentioning

confidence: 99%

An ultrasensitive enzyme-free electrochemical immunosensor based on redox cycling amplification using methylene blue

Dutta

Lillehoj

2017

Analyst

View full text Add to dashboard Cite

We report a new enzyme-free electrochemical sensor for ultrasensitive measurements of protein biomarkers in plasma and whole blood samples based on a unique electrochemical-chemical-chemical (ECC) redox cycling signal amplification scheme. This scheme uses methylene blue (MB) as a redox indicator which undergoes an endergonic reaction with Ru(NH3)63+ and a highly exergonic reaction with tris(2-carboxyethyl)phosphine (TCEP). This approach offers improved detection sensitivity and sensor stability compared with enzyme-based ECC redox cycling techniques while involving a simpler sensor modification process and detection protocol. This redox cycling scheme was combined with a robust immunosandwich assay for quantitative measurements of protein biomarkers. For proof of principle, Plasmodium falciparum histidine-rich protein 2 (PfHRP2) was measured in human plasma and whole blood samples, which could be detected down to 10 fg/mL and 18 fg/mL, respectively. Furthermore, this immunosensor exhibits high selectivity, excellent reproducibility and good stability for up to 2 weeks, making it a promising platform for point-of-care testing, especially for detecting extremely low biomarker concentrations in raw biofluids.

show abstract

Graphene-Orange II composite nanosheets with electroactive functions as label-free aptasensing platform for “signal-on” detection of protein

Cited by 46 publications

References 43 publications

Aptamer-Based Electrochemical Sensing of Lysozyme

Aptamer-Based Electrochemical Sensing of Lysozyme

Dual aptamer-immobilized surfaces for improved affinity through multiple target binding in potentiometric thrombin biosensing

An ultrasensitive enzyme-free electrochemical immunosensor based on redox cycling amplification using methylene blue

Contact Info

Product

Resources

About