Graphene oxide: A growth factor delivery carrier to enhance chondrogenic differentiation of human mesenchymal stem cells in 3D hydrogels

Zhou, Mi; Lozano, Neus; Wychowaniec, Jacek K.; Hodgkinson, Tom; Richardson, Stephen M.; Kostarelos, Kostas; Hoyland, Judith A.

doi:10.1016/j.actbio.2019.07.027

Cited by 117 publications

(92 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GO/PRP Composite Scaffold could enhance TBH's quality, improve the biomechanical strength and stiffness, restoring the biomechanical properties. This is in line with Wang's and Zhou's researches [20,37] that GO, as a carrier, possesses good osteogenic, chondrogenic, and angiogenic induction capabilities. The number of Sharpey's bers determines the tensile strength of the TBI.…”

Section: Discussionsupporting

confidence: 87%

Enhancement of Rotator Cuff Tendon-bone Healing with Graphene Oxide/PRP Composite Scaffold in a Rabbit Model: A Histological and Biomechanical Study

Qin¹,

Bao²,

Zeng³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Application of platelet-rich plasma (PRP) can improve tendon-bone healing (TBH) after rotator cuff surgical repair. Graphene Oxide (GO) is a steady, controlled, and sustained carrier. The purpose of this study is to determine whether GO/PRP Composite Scaffold enhances the TBH after RC surgical repair in a rabbit model.Methods: A full-thickness tear of the supraspinatus tendon was created and repaired in 36 adult male New Zealand rabbits. They were divided into three groups: Control group, PRP group, and GO/PRP Composite Scaffold group (GO group). The effect of GO/PRP Composite Scaffold on TBH was assessed using histological and biomechanical evaluations at 8 and 12 weeks postoperatively.Results: Histological analysis showed that greater continuity, better orientation, and more density of collagen fiber were detected in the GO group than PRP and Control groups at 8 and 12 weeks, respectively. Results of biomechanical evaluations showed that the load to failure and stiffness of the GO group were statistically higher than those of PRP and Control groups at both 8 and 12 weeks (P<0.05). Compared with 8 weeks in the GO group, there was no significant difference in load to failure at 12 weeks (P>0.05), while the stiffness at 12 weeks was higher than that at 8 weeks (P<0.05). Conclusions: These results demonstrated that GO/PRP Composite Scaffold enhanced the TBH following rotator cuff surgical repair in a rabbit model. The GO may be an effective carrier for PRP into repair sites.

show abstract

Section: Discussionsupporting

confidence: 87%

Enhancement of Rotator Cuff Tendon-bone Healing with Graphene Oxide/PRP Composite Scaffold in a Rabbit Model: A Histological and Biomechanical Study

Qin¹,

Bao²,

Zeng³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The expressions of aggrecan, glycosaminoglycan, type‐II collagen (Col II), and SOX‐9 were obviously increased. [ 121 ] These results were verified by later studies [ 122–124 ] in which MSCs and GO‐TGF‐ β 3 biocomposites were folded and assembled into composite pellets, [ 123 ] as illustrated in Figure a. The GO–TGF‐ β 3 enhanced the differentiation of MSCs and stimulated cartilage matrix elaboration.…”

Section: Cartilage Repairsupporting

confidence: 55%

Section: Cartilage Repairmentioning

confidence: 99%

2D Nanomaterials for Tissue Engineering and Regenerative Nanomedicines: Recent Advances and Future Challenges

Zheng

Hong

Wang

et al. 2021

Adv Healthcare Materials

117

View full text Add to dashboard Cite

Regenerative medicine has become one of the hottest research topics in medical science that provides a promising way for repairing tissue defects in the human body. Due to their excellent physicochemical properties, the application of 2D nanomaterials in regenerative medicine has gradually developed and has been attracting a wide range of research interests in recent years. In particular, graphene and its derivatives, black phosphorus, and transition metal dichalcogenides are applied in all the aspects of tissue engineering to replace or restore tissues. This review focuses on the latest advances in the application of 2D‐nanomaterial‐based hydrogels, nanosheets, or scaffolds that are engineered to repair skin, bone, and cartilage tissues. Reviews on other applications, including cardiac muscle regeneration, skeletal muscle repair, nerve regeneration, brain disease treatment, and spinal cord healing are also provided. The challenges and prospects of applications of 2D nanomaterials in regenerative medicine are discussed.

show abstract

“…Besides, to encourage stem cell-scaffold interactions, many immobilized biological components are often used to decorate materials. In addition to the nature of the scaffold material, antibodies, protein modifications-including with epidermal growth factor (EGF), neurotrophin-3 (NT-3), and glial cell-derived neurotrophic factor (GDNF)-can be applied to the scaffold to regulate stem cell fate [54]. They can provide an avenue for continuous growth factor delivery, which can alter the environment, making it more conducive for regeneration.…”

Section: Combination Of Biomaterials and Mscs Improve The Microenvironmentioning

confidence: 99%

Biomaterial-supported MSC transplantation enhances cell–cell communication for spinal cord injury

Zhang

Yuan

et al. 2021

Stem Cell Res Ther

View full text Add to dashboard Cite

The spinal cord is part of the central nervous system (CNS) and serves to connect the brain to the peripheral nervous system and peripheral tissues. The cell types that primarily comprise the spinal cord are neurons and several categories of glia, including astrocytes, oligodendrocytes, and microglia. Ependymal cells and small populations of endogenous stem cells, such as oligodendrocyte progenitor cells, also reside in the spinal cord. Neurons are interconnected in circuits; those that process cutaneous sensory input are mainly located in the dorsal spinal cord, while those involved in proprioception and motor control are predominately located in the ventral spinal cord. Due to the importance of the spinal cord, neurodegenerative disorders and traumatic injuries affecting the spinal cord will lead to motor deficits and loss of sensory inputs.Spinal cord injury (SCI), resulting in paraplegia and tetraplegia as a result of deleterious interconnected mechanisms encompassed by the primary and secondary injury, represents a heterogeneously behavioral and cognitive deficit that remains incurable. Following SCI, various barriers containing the neuroinflammation, neural tissue defect (neurons, microglia, astrocytes, and oligodendrocytes), cavity formation, loss of neuronal circuitry, and function must be overcame. Notably, the pro-inflammatory and anti-inflammatory effects of cell–cell communication networks play critical roles in homeostatic, driving the pathophysiologic and consequent cognitive outcomes. In the spinal cord, astrocytes, oligodendrocytes, and microglia are involved in not only development but also pathology. Glial cells play dual roles (negative vs. positive effects) in these processes. After SCI, detrimental effects usually dominate and significantly retard functional recovery, and curbing these effects is critical for promoting neurological improvement. Indeed, residential innate immune cells (microglia and astrocytes) and infiltrating leukocytes (macrophages and neutrophils), activated by SCI, give rise to full-blown inflammatory cascades. These inflammatory cells release neurotoxins (proinflammatory cytokines and chemokines, free radicals, excitotoxic amino acids, nitric oxide (NO)), all of which partake in axonal and neuronal deficit.Given the various multifaceted obstacles in SCI treatment, a combinatorial therapy of cell transplantation and biomaterial implantation may be addressed in detail here. For the sake of preserving damaged tissue integrity and providing physical support and trophic supply for axon regeneration, MSC transplantation has come to the front stage in therapy for SCI with the constant progress of stem cell engineering. MSC transplantation promotes scaffold integration and regenerative growth potential. Integrating into the implanted scaffold, MSCs influence implant integration by improving the healing process. Conversely, biomaterial scaffolds offer MSCs with a sheltered microenvironment from the surrounding pathological changes, in addition to bridging connection spinal cord stump and offering physical and directional support for axonal regeneration. Besides, Biomaterial scaffolds mimic the extracellular matrix to suppress immune responses.Here, we review the advances in combinatorial biomaterial scaffolds and MSC transplantation approach that targets certain aspects of various intercellular communications in the pathologic process following SCI. Finally, the challenges of biomaterial-supported MSC transplantation and its future direction for neuronal regeneration will be presented.

show abstract

Graphene oxide: A growth factor delivery carrier to enhance chondrogenic differentiation of human mesenchymal stem cells in 3D hydrogels

Cited by 117 publications

References 39 publications

Enhancement of Rotator Cuff Tendon-bone Healing with Graphene Oxide/PRP Composite Scaffold in a Rabbit Model: A Histological and Biomechanical Study

Enhancement of Rotator Cuff Tendon-bone Healing with Graphene Oxide/PRP Composite Scaffold in a Rabbit Model: A Histological and Biomechanical Study

2D Nanomaterials for Tissue Engineering and Regenerative Nanomedicines: Recent Advances and Future Challenges

Biomaterial-supported MSC transplantation enhances cell–cell communication for spinal cord injury

Contact Info

Product

Resources

About