Graphene oxide accelerates TGFβ-mediated epithelial-mesenchymal transition and stimulates pro-inflammatory immune response in amniotic epithelial cells

Cerveró-Varona, Adrián; Canciello, Angelo; Peserico, Alessia; Haidar, Arlette; Citeroni, Maria Rita; Mauro, Annunziata; Russo, Valentina; Moffa, Samanta; Pilato, Serena; Giacomo, Stefano Di; Dufrusine, Beatrice; Dainese, Enrico; Fontana, Antonella; Barboni, Barbara

doi:10.1016/j.mtbio.2023.100758

Cited by 5 publications

(1 citation statement)

References 63 publications

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“…We acknowledge that the addition of 3-MA did not have a strong effect on alleviating cytotoxicity. Previous studies have shown that nanosheets can trigger various intracellular biological events, such as oxidative stress [58] and inflammatory responses [59]. We speculated that, in addition to autophagy, various intracellular biological events were involved in the enhanced sensitivity of A549 cells to DOX induced by WS2 and WSe2 nanosheets.…”

Section: Ws2 and Wse2 Sensitized A549 Cells To Dox By Triggering Auto...mentioning

confidence: 93%

Enhanced Sensitivity of A549 Cells to Doxorubicin with WS2 and WSe2 Nanosheets via the Induction of Autophagy

Jin,

Yang,

Jia

et al. 2024

IJMS

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The excellent physicochemical properties of two-dimensional transition-metal dichalcogenides (2D TMDCs) such as WS2 and WSe2 provide potential benefits for biomedical applications, such as drug delivery, photothermal therapy, and bioimaging. WS2 and WSe2 have recently been used as chemosensitizers; however, the detailed molecular basis underlying WS2- and WSe2-induced sensitization remains elusive. Our recent findings showed that 2D TMDCs with different thicknesses and different element compositions induced autophagy in normal human bronchial epithelial cells and mouse alveolar macrophages at sublethal concentrations. Here, we explored the mechanism by which WS2 and WSe2 act as sensitizers to increase lung cancer cell susceptibility to chemotherapeutic agents. The results showed that WS2 and WSe2 enhanced autophagy flux in A549 lung cancer cells at sublethal concentrations without causing significant cell death. Through the autophagy-specific RT2 Profiler PCR Array, we identified the genes significantly affected by WS2 and WSe2 treatment. Furthermore, the key genes that play central roles in regulating autophagy were identified by constructing a molecular interaction network. A mechanism investigation uncovered that WS2 and WSe2 activated autophagy-related signaling pathways by interacting with different cell surface proteins or cytoplasmic proteins. By utilizing this mechanism, the efficacy of the chemotherapeutic agent doxorubicin was enhanced by WS2 and WSe2 pre-treatment in A549 lung cancer cells. This study revealed a feature of WS2 and WSe2 in cancer therapy, in which they eliminate the resistance of A549 lung cancer cells against doxorubicin, at least partially, by inducing autophagy.

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Section: Ws2 and Wse2 Sensitized A549 Cells To Dox By Triggering Auto...mentioning

confidence: 93%

Enhanced Sensitivity of A549 Cells to Doxorubicin with WS2 and WSe2 Nanosheets via the Induction of Autophagy

Jin,

Yang,

Jia

et al. 2024

IJMS

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Graphene Oxide: A Promising Nanomaterial for Antibacterial and Antiviral Applications

Quezada,

Congreve,

Kokkarachedu

2024

Nanotechnology in the Life Sciences

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Optimization of a nanoparticle uptake protocol applied to amniotic-derived cells: unlocking the therapeutic potential

Peserico,

Canciello,

Prencipe

et al. 2024

J. Mater. Chem. B

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Graphene oxide accelerates TGFβ-mediated epithelial-mesenchymal transition and stimulates pro-inflammatory immune response in amniotic epithelial cells

Cited by 5 publications

References 63 publications

Enhanced Sensitivity of A549 Cells to Doxorubicin with WS2 and WSe2 Nanosheets via the Induction of Autophagy

Enhanced Sensitivity of A549 Cells to Doxorubicin with WS2 and WSe2 Nanosheets via the Induction of Autophagy

Graphene Oxide: A Promising Nanomaterial for Antibacterial and Antiviral Applications

Optimization of a nanoparticle uptake protocol applied to amniotic-derived cells: unlocking the therapeutic potential

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