Graphene Oxide and Metal Particles Nanocomposites for Inhibition of Pathogenic Bacteria Strains

Abstract

The most effective means to protect against bacterial invasion and to reduce the risk of dangerous infections are antibacterial components synthesis. Preliminary investigations show that hybrid coatings of graphene oxide doped with silver and copper nanoparticles have long-term bactericidal effect on a wide spectrum of standard bacterial strains. In this study the possibilities to use hybrid graphene oxide and metal nanoparticles composites for the inhibition not only standard bacteria, but also clinically important bacteria strains, which have developed multiple resistances to antibiotics, have been investigated. Resistant to antibiotics bacteria often cause dangerous infections in medical institutions of many countries due to the acquired resistance to many classes of antibiotics and the ability to obtain resistance to all antibiotics through mutations. Nanocomposite samples were characterized by TEM and SEM microscopy. The antibacterial activity was evaluated against gram-negative, gram-positive and clinical antibiotics resistant A. baumannii bacteria strains by solutions dilution method. The investigations demonstrated that hybrid nanocomposite shows synergistic mechanism of action and characterizes high efficiency against multidrug-resistant bacteria, such as A. baumannii bacteria strains decrease of viability.

<p>Effect of Graphene Oxide and Silver Nanoparticles Hybrid Composite on P. aeruginosa Strains with Acquired Resistance Genes</p>

“…To determine the bacterial growth inhibition of GO-Ag hybrid nanocomposite to carbapenem-resistant bacteria we used previously developed methodology. 38 P. aeruginosa strains were cultivated overnight on 5% blood agar (BD, USA) at a temperature of 37°C. Saline was used to make P. aeruginosa inoculate of 0.5 MF concentration.…”