Graphene oxide-assisted optimized narrow-thermal-cycling amplification for accurate detection of Salmonella spp.

Rapid diagnosis of acute myocardial infarction based on reverse transcription-accelerated strand exchange amplification of miR-208a

“…The new product will serve as the template for the next amplification, causing the 3′ terminal bases of the amplification product to continuously accumulate. 39–41…”

“…The new product will serve as the template for the next amplication, causing the 3 ′ terminal bases of the amplication product to continuously accumulate. [39][40][41] To realize the rapid amplication and detection of miR-208a, the RT-ASEA method was preliminarily established based on the designed reaction system and amplication program. In order to reect the effective improvement of the narrow cycle for the amplication efficiency of RT-ASEA, the same reaction system was reacted at a constant temperature of 60 °C for 1 h. Fig.…”

Accurate Identification of Salmonella enterica in Calves

“…Environmental samples are another type of sample that can be collected for S. enterica detection in cattle, especially in cases of environmental monitoring or investigation [31,50,51]. Environmental samples can include water, soil, bedding, feed, equipment, and other materials that may be contaminated by S. enterica [33].…”

“…A study by the author [50] compared the culture and PCR methods for S. enterica detection in fecal samples of beef cattle in Nebraska, USA. The study found that the culture method detected S. enterica in 6.3% of the samples, while the PCR method detected S. enterica in 12.5% of the samples.…”

“…XLD is used as a primary isolation medium for S. enterica detection in fecal, food, and environmental samples. On XLD, S. enterica colonies appear as red colonies with or without a black center, while other bacteria appear as yellow colonies with or without a black center or are inhibited [50]. Hektoen enteric agar (HE): This is a selective and differential medium that contains lactose, sucrose, and salicin, which inhibit the growth of most Gram-positive and some Gram-negative bacteria, while allowing the growth of S. enterica and some other enteric bacteria.…”