Graphene oxide based fluorescence resonance energy transfer and loop-mediated isothermal amplification for white spot syndrome virus detection

Waiwijit, Uraiwan; Phokaratkul, D.; Kampeera, Jantana; Lomas, Tanom; Wisitsoraat, Anurat; Kiatpathomchai, Wansika; Tuantranont, Adisorn

doi:10.1016/j.jbiotec.2015.08.003

Cited by 25 publications

(12 citation statements)

References 30 publications

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“…The probe is unquenched and fluorescence can be detected. Graphene Oxide based FRET LAMP 42 is another approach that uses a fluorescent probe, but with graphene oxide quenching the DNA fluorophore complex. Quenching of unincorporated amplification signal reporters (QUASR) 43 offers an alternative approach for increased specificity using temperature to determine the presence of target.…”

Section: Introductionmentioning

confidence: 99%

Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Hardinge

Murray

2019

Sci Rep

149

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Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular diagnostics as an alternative to PCR based methods. There are numerous reported techniques to detect the LAMP amplification including turbidity, bioluminescence and intercalating fluorescent dyes. In this report we show that quenched fluorescent labels on various LAMP primers can be used to quantify and detect target DNA molecules down to single copy numbers. By selecting different fluorophores, this method can be simply multiplexed. Moreover this highly specific LAMP detection technique can reduce the incidence of false positives originating from mispriming events. Attribution of these events to particular primers will help inform and improve LAMP primer design.

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Section: Introductionmentioning

confidence: 99%

Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Hardinge

Murray

2019

Sci Rep

149

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“…FRET between the uorophore and GO was rst applied by Waiwijit et al to detect white spot syndrome virus. 67 Analytical sensitivity was 10 plasmid DNA copies per reaction and 0.6 fg infected shrimp total DNA. High analytical specicity (100%) was conrmed by testing against common shrimp viruses.…”

Section: Alternately Binding Quenching Probe Competitive Lamp (Abc-lamp)mentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

Becherer

Borst

Bakheit³

et al. 2020

Anal. Methods

311

245

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“…These features can be utilized in biosensor construction ( Lu et al, 2009 ). The selectivity and detection limit in the FRET detection mode depend not only on the ability for Watson–Crick base-pairing but also the noncovalent binding of nucleobases to nanomaterials ( Iliafar et al, 2014 ; Umadevi and Sastry, 2011 ; Waiwijit et al, 2015 ). It should be noted that the strength of interaction of biomacromolecules and their building blocks to 2D nanomaterials is still largely underexplored with respect to binding affinities among biomolecules.…”

Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

161

117

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Our recent experience of the COVID-19 pandemic has highlighted the importance of easy-to-use, quick, cheap, sensitive and selective detection of virus pathogens for the efficient monitoring and treatment of virus diseases. Early detection of viruses provides essential information about possible efficient and targeted treatments, prolongs the therapeutic window and hence reduces morbidity. Graphene is a lightweight, chemically stable and conductive material that can be successfully utilized for the detection of various virus strains. The sensitivity and selectivity of graphene can be enhanced by its functionalization or combination with other materials. Introducing suitable functional groups and/or counterparts in the hybrid structure enables tuning of the optical and electrical properties, which is particularly attractive for rapid and easy-to-use virus detection. In this review, we cover all the different types of graphene-based sensors available for virus detection, including, e.g., photoluminescence and colorimetric sensors, and surface plasmon resonance biosensors. Various strategies of electrochemical detection of viruses based on, e.g., DNA hybridization or antigen-antibody interactions, are also discussed. We summarize the current state-of-the-art applications of graphene-based systems for sensing a variety of viruses, e.g., SARS-CoV-2, influenza, dengue fever, hepatitis C virus, HIV, rotavirus and Zika virus. General principles, mechanisms of action, advantages and drawbacks are presented to provide useful information for the further development and construction of advanced virus biosensors. We highlight that the unique and tunable physicochemical properties of graphene-based nanomaterials make them ideal candidates for engineering and miniaturization of biosensors.

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Graphene oxide based fluorescence resonance energy transfer and loop-mediated isothermal amplification for white spot syndrome virus detection

Cited by 25 publications

References 30 publications

Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection

Human virus detection with graphene-based materials

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