Graphene oxide-based substrate: physical and surface characterization, cytocompatibility and differentiation potential of dental pulp stem cells

Rosa, Vinícius; Xie, Han; Dubey, Nileshkumar; Madanagopal, Thulasi Thiruvallur; Rajan, Sneha Sundar; Morin, Julien; Islam, Intekhab; Neto, A. H. Castro

doi:10.1016/j.dental.2016.05.008

Cited by 113 publications

(75 citation statements)

References 29 publications

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“…From curve a, it is clear that there is a characteristic peak belonging to manganese oxide at 610 cm −1 , while, the characteristic peaks at 1380 cm −1 and 1598 cm −1 are related to D and G bands, respectively. The D characteristic peak is related to the degree of graphene defect, and the G characteristic peak is the in‐plane vibration of sp 2 carbon atoms in graphene and the ratio of I D / I G reflects the degree of defect in the prepared rGO . Thus, the I D / I G values for Pd/rGO (1.08) and Pd−Mn/rGO (1.05) are not much different, which reveals that the addition of Mn has no obvious influence on the disorder of graphene during the preparation of the material.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Non‐enzymatic Electrochemical Glucose Sensor Based on Pd−Mn Alloy Nanoparticles Supported on Reduced Graphene Oxide

et al. 2020

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Mixed metals alloy nanoparticles supported on carbon nanomaterial are the most attractive candidates for the fabrication of non‐enzymatic electrochemical sensor with enhanced electrochemical performance. In this study, palladium‐manganese alloy nanoparticles supported on reduced graphene oxide (Pd−Mn/rGO) are prepared by a simple reduction protocol. Further, a novel enzyme‐free glucose sensing platform is established based on Pd−Mn/rGO. The successful fabrication of Pd−Mn alloy nanoparticles and their attachment at rGO are thoroughly characterized by various microscopic and spectroscopic techniques such as XRD, Raman, TEM and XPS. The electrochemical activity and sensing features of designed material towards glucose detection are explored by amperometric measurments in 0.1 M NaOH at the working voltage of −0.1 V. Thanks to the newly designed Pd−Mn/rGO nanohybrid for their superior electrorochemical activity towards glucose comprising the admirable sensing features in terms of targeted selectivity, senstivity, two linear parts and good stability. The enhanced electrochemical efficacy of Pd−Mn/rGO electrocatalyst may be credited to the abundant elecrocatalytic active sites formed during the Pd−Mn alloying and the electron transport ability of rGO that augment the electron shuttling phenomenon between the electrode material and targeted analyte.

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Section: Resultsmentioning

confidence: 99%

Fabrication of Non‐enzymatic Electrochemical Glucose Sensor Based on Pd−Mn Alloy Nanoparticles Supported on Reduced Graphene Oxide

et al. 2020

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“…The use of human dental pulp stem cells (DPSC) from single donor (DPF003, Allcells, Alameda) was approved by NUS Institutional Review Board (Approval Number: NUS 2094). The characterization and expressions of CD34, CD73, CD90, CD105 are available elsewhere (Rosa et al, ). The cells were cultured in DMEM, supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (all from Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

Taguchi's methods to optimize the properties and bioactivity of 3D printed polycaprolactone/mineral trioxide aggregate scaffold: Theoretical predictions and experimental validation

et al. 2019

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Mineral trioxide aggregate (MTA) can provide bioactivity to poly‐caprolactone (PCL), which is an inert polymer used to print scaffolds. However, testing all combinations of scaffold characteristics (e.g., composition, pore size, and distribution) to optimize properties of scaffolds is time‐consuming and costly. The Taguchi's methods can identify characteristics that have major influences on the properties of complex designs, hence decreasing the number of combinations to be tested. The objective was to assess the potential of Taguchi's methods as a predictive tool for the optimization of bioactive scaffold printed using electro‐hydro dynamic jetting. A three‐level approach assessed the influence of PCL/MTA proportion, pore size, fiber dimension and number of layers in pH, degradation rate, porosity, yield strength, and Young's modulus. Data were analyzed using Tukey's honest significant difference test, analysis of mean and signal‐to‐noise ratio (S/N) test. Cytocompatibility and differentiation potential were assessed for 5 and 30 days using dental pulp stem cells and analyzed with one‐way analysis of variance (proliferation) or Mann–Whitney (qPCR). The S/N ratio and analysis of mean showed that fiber diameter and composition were the most influential characteristics in all properties. The experimental data confirmed that the addition of MTA to PCL increased the pH and scaffold degradation. Only PCL and PCL with 4% MTA allowed cell proliferation. The latter increased the genetic expression of ALP, COL‐1, OCN, and MSX‐1. The theoretical predictions were confirmed by the experiments. The Taguchi's identified the inputs that can be disregarded to optimize 3D printed meshed bioactive scaffolds.

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“…Published works have suggested that GO has good biocompatibility. Rosa V et al showed that DPSCs were anchored to the rough surface provided by GO, as assessed by scanning electron microscopy (SEM) [26]. Moreover, GO at the concentration of 0.1 lg/ml allowed for BMSC attachment and promoted proliferation, whilst GO at 10 lg/ml was cytotoxic [27].…”

Section: Introductionmentioning

confidence: 99%

Effects of graphene oxide and graphene oxide quantum dots on the osteogenic differentiation of stem cells from human exfoliated deciduous teeth

Yang

Zhao

Chen

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Graphene and its derivatives, graphene oxide (GO) and graphene oxide quantum dots (GOQDs), have recently attracted much attention as bioactive factors in differentiating stem cells towards osteoblastic lineage. The stem cells from human exfoliated deciduous teeth (SHEDs) possess the properties of selfrenewal, extensive proliferation, and multiple differentiation potential, and have gradually become one of the most promising mesenchymal stem cells (MSCs) in bone tissue engineering. The purpose of this study was to explore the effects of GO and GOQDs on the osteogenic differentiation of SHEDs. In this study, GO and GOQDs facilitated SHED proliferation up to 7 days in vitro at the concentration of 1 lg/ml. Because of their excellent fluorescent properties, GOQD uptake by SHEDs was confirmed and distributed in the SHED cytoplasm. Calcium nodules formation, alkaline phosphatase (ALP) activity, and RNA and protein expression increased significantly in SHEDs treated with osteogenic induction medium containing GOQDs but decreased with osteogenic induction medium containing GO. Interestingly, the Wnt/b-catenin signaling pathway appeared to be involved in osteogenic differentiation of SHEDs induced with GOQDs. In summary, GO and GOQDs at the concentration of 1 lg/ml promoted SHED proliferation. GOQDs induced the osteogenic differentiation of SHEDs, whilst GO slightly inhibited it.

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Graphene oxide-based substrate: physical and surface characterization, cytocompatibility and differentiation potential of dental pulp stem cells

Cited by 113 publications

References 29 publications

Fabrication of Non‐enzymatic Electrochemical Glucose Sensor Based on Pd−Mn Alloy Nanoparticles Supported on Reduced Graphene Oxide

Fabrication of Non‐enzymatic Electrochemical Glucose Sensor Based on Pd−Mn Alloy Nanoparticles Supported on Reduced Graphene Oxide

Taguchi's methods to optimize the properties and bioactivity of 3D printed polycaprolactone/mineral trioxide aggregate scaffold: Theoretical predictions and experimental validation

Effects of graphene oxide and graphene oxide quantum dots on the osteogenic differentiation of stem cells from human exfoliated deciduous teeth

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