Graphene Oxide Biosensors Based on Hybridization Chain Reaction Signal Amplification for Detecting Biomarkers of Radiation-Resistant Nasopharyngeal Carcinoma and Imaging in Living Cells

Lan, Qing; Yang, Dutao; Yin, Shaoxian; Qian, Yue; Cai, Yanfei; Jin, Jian; Huang, Gang; Yang, Zhaoqi

doi:10.1021/acs.langmuir.1c00406

Cited by 12 publications

(6 citation statements)

References 37 publications

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“…[47,65] Yang et al designed a target (miRNA-205)-triggered HCR-based fluorescent probe, which enabled linear signal amplification for the detection of intracellular miRNA-205. [66] Na and co-workers constructed DNA-inorganic hybrid nanowire (NW) balls using HCR-programmable hairpin oligonucleotide probes (H1, H2) and Zn 2 + ions through a one-pot reaction. When the nucleic acid probe was delivered into cells by the NW balls, intracellular target (e. g. miR-21) specifically hybridised with H1, triggering HCR-amplified sensing and realising the in situ visualisation of low-abundance targets (Figure 5A).…”

Section: Hcr-mediated Signal Amplificationmentioning

confidence: 99%

“…Yang et al . designed a target (miRNA‐205)‐triggered HCR‐based fluorescent probe, which enabled linear signal amplification for the detection of intracellular miRNA‐205 [66] . Na and co‐workers constructed DNA–inorganic hybrid nanowire (NW) balls using HCR‐programmable hairpin oligonucleotide probes (H1, H2) and Zn 2+ ions through a one‐pot reaction.…”

Section: Non‐enzymatic Amplificationmentioning

confidence: 99%

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Intracellular Signal Amplification for Ultrasensitive Detection and Imaging: Progress, Challenges, and Opportunities

Gong

Shan

et al. 2022

Analysis & Sensing

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Disease signaling molecules and biomarkers are important in biosensing and biomedicine, and their ultra‐sensitive detection and imaging are critical in medical diagnosis. However, most biomarkers have low levels of expression in cells and are easily degraded; therefore, they cannot be accurately detected in real time by conventional amplification methods, which are typically confined to cell lysates. To achieve highly sensitive detection of intracellular low‐abundance molecules, extensive efforts have been devoted to the development of in situ signal amplification techniques. This review tracks the development of recent advances in in situ signal amplification strategies, and systematically summarizes the advantages and disadvantages of enzymatic and non‐enzymatic amplification techniques for detecting and tracing intracellular targets. Further, perspectives, challenges, and opportunities for intracellular signal amplification are discussed to promote its adoption for in situ signal amplification in clinical settings and to provide new insights into signaling molecules and biomarkers in cellular functions and associated diseases.

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Section: Hcr-mediated Signal Amplificationmentioning

confidence: 99%

Section: Non‐enzymatic Amplificationmentioning

confidence: 99%

Intracellular Signal Amplification for Ultrasensitive Detection and Imaging: Progress, Challenges, and Opportunities

Gong

Shan

et al. 2022

Analysis & Sensing

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“…However, the sensitivity of DNA nanotweezers is not sufficient to meet the diagnostic requirements. Signal amplification techniques such as a hybridization chain reaction and rolling circle amplification are not ideal because they need a continuous input of either fuel strands or enzymes. ,− Among all different designs of DNA nanostructures, a two-dimensional DNA tile is able to self-repeat into a large-scale structure. − We designed nine oligomers that self-assemble into a four-arm DNA junction . Two individual tiles, named TA and TB (Figure S1), were adopted in this study .…”

Section: Introductionmentioning

confidence: 99%

Self-Assembly of DNA Tiles with G-Quadruplex DNAzyme Catalytic Activity for Sensing Applications

Wang

Cui

Tanner

et al. 2022

ACS Appl. Bio Mater.

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DNA tiles form through self-assembly of a small number of DNA strands that interact through basic repeated interactions, allowing the growth of nanoscale structures seeded by molecular inputs. If an approach for catalytic signal amplification can be integrated into the resultant nanostructure, then one can anticipate biosensing or diagnostic applications mediated by DNA tile self-assembly. Here, two-dimensional DNA tiles with split quadruplexes were designed as diagnostic tools for nucleic acid sensing without the use of protein enzymes. The presence of a target sequence leads to formation of extended microscale structures with arrayed multiple G-quadruplexes across the tile plane, with catalytic activity coupled to a colorimetric reporter. Such a mechanism has potential for low-cost signal amplification using unmodified DNA without the use of protein enzymes for biosensing.

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“…Hybridization chain reaction (HCR) as one label-free nucleic acid amplification approach has attracted substantial research efforts because it is simple, cost-effective, and enzyme-free. Many detection strategies based on HCR have been developed for the analysis of DNA, RNA, proteins, and small-molecular-weight targets. − However, the detection sensitivity of the assays based on HCR is not high enough for the detection of targets at trace concentration because it is a linear signal-amplification method. Therefore, an additional nucleic acid amplification procedure is needed to improve the detection sensitivity in a biological assay.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Detection of the BRCA1 Gene via Exponential Isothermal Amplification and Hybridization Chain Reaction of Surface-Bound Probes

et al. 2022

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In this work, we demonstrated an ultrasensitive approach with a dual-amplification strategy for DNA assay based on isothermal exponential amplification (EXPAR) and the hybridization chain reaction (HCR). In the presence of target DNA, the hairpin probe DNA (HP1) recognized and partially hybridized with the target DNA to form double-stranded structures containing the full recognition sequences for nicking endonuclease and then initiated EXPAR. Under the reaction of EXPAR, a large number of single-stranded DNA (ssDNA) was produced in the circle of nicking, polymerization, and strand displacement. The resulting ssDNA can bind to the surface-bound probe on the well of the microplate and trigger the hybridization chain reaction, resulting in the production of numerous double-stranded DNA concatamers with biotin labeling. In the presence of streptavidin-conjugated horseradish peroxidase (HRP), the amplified signal can be detected by a spectrophotometer via HRP-catalyzed substrate 3,3′5,5′-tetramethylbenzidine (TMB). This proposed dualamplification method provides a detection limit of 74.48 aM, which also exhibits good linearity ranging from 0.1 fM to 100 pM.

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Graphene Oxide Biosensors Based on Hybridization Chain Reaction Signal Amplification for Detecting Biomarkers of Radiation-Resistant Nasopharyngeal Carcinoma and Imaging in Living Cells

Cited by 12 publications

References 37 publications

Intracellular Signal Amplification for Ultrasensitive Detection and Imaging: Progress, Challenges, and Opportunities

Intracellular Signal Amplification for Ultrasensitive Detection and Imaging: Progress, Challenges, and Opportunities

Self-Assembly of DNA Tiles with G-Quadruplex DNAzyme Catalytic Activity for Sensing Applications

Spectrophotometric Detection of the BRCA1 Gene via Exponential Isothermal Amplification and Hybridization Chain Reaction of Surface-Bound Probes

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