Graphene Oxide/Gelatin Nanofibrous Scaffolds Loaded with N-Acetyl Cysteine for Promoting Wound Healing

Abstract

Purpose We aimed to develop an antioxidant dressing material with pro-angiogenic potential that could promote wound healing. Gelatin (Gel) was selected to improve the biocompatibility of the scaffolds, while graphene oxide (GO) was added to enhance their mechanical property. The loaded N-Acetyl cysteine (NAC) was performing the effect of scavenging reactive oxygen species (ROS) at the wound site. Materials and Methods The physicochemical and mechanical properties, NAC releases, and biocompatibility of the NAC-GO-Gel scaffolds were evaluated in vitro. The regeneration capability of the scaffolds was systemically investigated in vivo using the excisional wound-splinting model in mice. Results The NAC-GO-Gel scaffold had a stronger mechanical property and sustainer NAC release ability than the single Gel scaffold, which resulted in a better capacity for cell proliferation and migration. Mice wound-splinting models revealed that the NAC-GO-Gel scaffold effectively accelerated wound healing, promoted re-epithelialization, enhanced neovascularization, and reduced scar formation. Conclusion The NAC-GO-Gel scaffold not only promotes wound healing but also reduces scar formation, showing a great potential application for the repair of skin defects.

Gelatin-based biomaterials and gelatin as an additive for chronic wound repair

“…Stem cells can promote the production of transforming growth factor-β3 (TGF-β3), VEGF, and insulin growth factor (IGF) through paracrine, promoting cell migration and vascular regeneration ( Chen et al, 2021 ). Yu et al, 2023 reduced graphene oxide (GOG) by gelatin to increase its compressive strength for the delivery of N-acetylcysteine (NAC) to obtain NAC-GO-Gel. GO-Gel stents have good biocompatibility and promote neovascularization ( Shen et al, 2022 ).…”

Recent advancements in natural polymers‐based self‐healing nano‐materials for wound dressing

“…Gelatin (Gel) was chosen to improve scaffold biocompatibility, N ‐acetyl cysteine (NAC) was loaded into the scaffold to scavenge ROS at the wound site, while GO was incorporated to provide self‐healing and mechanical strength. Results demonstrated that the NAC‐GO‐Gel scaffold exhibited superior mechanical properties and sustained NAC release compared to Gel alone, leading to enhanced cell proliferation and migration 210 …”

Electrospun L-Lysine/Amorphous Calcium Phosphate Loaded Core-Sheath Nanofibers for Managing Oral Biofilm Infections and Promoting Periodontal Tissue Repairment

“…[29][30][31] On the other hand, electrospun nanofibers are constantly being used in new and innovative applications due to their ability to encapsulate active ingredients for specific purposes. [32][33][34] Additionally, it is easy to load multiple active ingredients into complex electrospun structures with the ability to tailor components, compositions, and spatial distributions within nanofibers. 35 As a result, this is a powerful method for achieving multiple functional benefits and achieving a designed synergistic effect.…”