Graphene oxide-hairpin probe nanocomposite as a homogeneous assay platform for DNA base excision repair screening

Zhou, Di; Xi, Qiang; Liang, Manfen; Chen, Cuihua; Tang, Li‐Juan; Jiang, Jian‐Hui

doi:10.1016/j.bios.2012.08.053

Cited by 54 publications

(21 citation statements)

References 37 publications

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“…GO has been used as a sensor for the detection of DNA 15 , proteins 16 and metal ions 17 ; as sorbent for solid-phase extraction 18 and 4 microextraction 19 . Recently it has been employed as adsorbent of heavy metals and organic compounds in the wastewater treatment 20 as well as novel carrier for drug delivery 21 .…”

Section: Introductionmentioning

confidence: 99%

Graphene Quantum Dots Sensor for the Determination of Graphene Oxide in Environmental Water Samples

2014

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The growing production and use of graphene and graphene-family nanomaterials will increase the human and environmental exposure to such nanomaterials, being necessary to evaluate their possible effects in the environment and, subsequently, on the ecosystem health, including humans. Graphene oxide (GO) possesses an unique structure composed of sp 2 carbons surrounded by sp 3 carbons and oxygen containing functional groups 1 which confer it an excellent aqueous solubility -hence their potential presence as contaminant of environmental waters-, surface functionalizability and fluorescence quenching ability.In this work, fluorescent graphene quantum dots (GQDs) have been employed to develop a simple, fast and sensitive fluorescence-based sensor for the determination of GO in natural river water samples 2 . GO is retained in acetate of cellulose membranes for their preconcentration being subsequently recovered in a water solution by ultrasound irradiation. The interaction of graphene oxide with GQDs through hydrophobic π-π stacking of the GO with the aromatic network at the GQDs surface leads to a decrease -quenching-of the fluorescence of GQDs, which was employed as analytical signal for their quantification. The limit of detection obtained was 35 µg·L -1 with a precision, for a 200 µg·L -1 concentration of GO of 5.16%.The procedure has been applied for the determination of GO in environmental river water samples. In such case a clean-up step was necessary in order to remove the interfering organic matter. The filtration of a sodium hydroxide solution which eliminated the retained organic matter overcame such interference, being possible to quantify the presence of GO obtaining good values of recoveries, ranging from 83.7% to 108.2% depending on the concentration level.In this work the two facets of Analytical Nanoscience and Nanotechnology -nanomaterials as analytical tools or objects-have been covered. GQDs are used as analytical tools owing to their exceptional fluorescence properties for the determination of GO in environmental samples.

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Section: Introductionmentioning

confidence: 99%

Graphene Quantum Dots Sensor for the Determination of Graphene Oxide in Environmental Water Samples

2014

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“…Using the electrochemical oxidation of guanine bases in released P1 at the graphene/GC electrode as the signal indicator, the detection UDG activity can be simply carried out with high sensitivity and selectivity. Comparing to reports in previous literature [13,14,[23][24][25]. There are no requirements to label the DNA strand and to construct the complex substrate electrode.…”

Section: Introductionmentioning

confidence: 63%

“…For example, Maksimenko et al carried out the detection of UDG activity by the fluorescence resonance energy transfer (FRET) probe [12,13]. Zhou et al detected UDG activity based on the interaction between a fluorophore-labeled hairpin DNA probe and graphene [14]. These strategies are effective for the lower detection limits.…”

Section: Introductionmentioning

confidence: 98%

A novel and label-free biosensors for uracil-DNA glycosylase activity based on the electrochemical oxidation of guanine bases at the graphene modified electrode

Jiao

Qian

Yun

et al. 2016

Talanta

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“…Similar to graphene-based assay [31], to avoid the potential interference of component in crude cell extract which may be adsorbed on the surface of WS 2 nanosheet or affect the Exo III activity, we used 450 mg mL À1 WS 2 nanosheet and 1.5 units Exo III in the experiments. As shown in Fig.…”

Section: The Assay Performance For Udg Activity Detectionmentioning

confidence: 99%

Tungsten disulfide nanosheet and exonuclease III co-assisted amplification strategy for highly sensitive fluorescence polarization detection of DNA glycosylase activity

Zhao

Kong

et al. 2015

Analytica Chimica Acta

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Graphene oxide-hairpin probe nanocomposite as a homogeneous assay platform for DNA base excision repair screening

Cited by 54 publications

References 37 publications

Graphene Quantum Dots Sensor for the Determination of Graphene Oxide in Environmental Water Samples

Graphene Quantum Dots Sensor for the Determination of Graphene Oxide in Environmental Water Samples

A novel and label-free biosensors for uracil-DNA glycosylase activity based on the electrochemical oxidation of guanine bases at the graphene modified electrode

Tungsten disulfide nanosheet and exonuclease III co-assisted amplification strategy for highly sensitive fluorescence polarization detection of DNA glycosylase activity

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