Graphene oxide incorporated polycaprolactone/chitosan/collagen electrospun scaffold: Enhanced osteogenic properties for bone tissue engineering

Aidun, Amir; Firoozabady, Alireza Safaei; Moharrami, Mohammad; Ahmadi, Ali; Haghighipour, Nooshin; Bonakdar, Shahin; Faghihi, Shahab

doi:10.1111/aor.13474

Cited by 81 publications

(56 citation statements)

References 73 publications

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“…This study aimed to reinforce CS scaffolds with GO at wt % of 2, 4, and 6 which were further crosslinked with glutaraldehyde (GTA). The highest GO wt % attempted in this study was justified by a recent work by Aidun and coworkers (2019) [ 22 ]. They also prepared their chitosan composite scaffolds with GO at wt % of 6.…”

Section: Introductionmentioning

confidence: 84%

Preliminary In Vitro Evaluation of Chitosan–Graphene Oxide Scaffolds on Osteoblastic Adhesion, Proliferation, and Early Differentiation

Wong

Lim

Tiong

et al. 2020

IJMS

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An ideal scaffold should be biocompatible, having appropriate microstructure, excellent mechanical strength yet degrades. Chitosan exhibits most of these exceptional properties, but it is always associated with sub-optimal cytocompatibility. This study aimed to incorporate graphene oxide at wt % of 0, 2, 4, and 6 into chitosan matrix via direct blending of chitosan solution and graphene oxide, freezing, and freeze drying. Cell fixation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, alkaline phosphatase colorimetric assays were conducted to assess cell adhesion, proliferation, and early differentiation of MG63 on chitosan–graphene oxide scaffolds respectively. The presence of alkaline phosphatase, an early osteoblast differentiation marker, was further detected in chitosan–graphene oxide scaffolds using western blot. These results strongly supported that chitosan scaffolds loaded with graphene oxide at 2 wt % mediated cell adhesion, proliferation, and early differentiation due to the presence of oxygen-containing functional groups of graphene oxide. Therefore, chitosan scaffolds loaded with graphene oxide at 2 wt % showed the potential to be developed into functional bone scaffolds.

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Section: Introductionmentioning

confidence: 84%

Preliminary In Vitro Evaluation of Chitosan–Graphene Oxide Scaffolds on Osteoblastic Adhesion, Proliferation, and Early Differentiation

Wong

Lim

Tiong

et al. 2020

IJMS

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“…Importantly, they can be combined with inorganic components, such as synthetic HAp, alpha-and beta-tricalcium phosphate (α-and β-TCP) as well as bioactive glass in order to improve their structural similarity to natural bone, mechanical properties, and osteoconductivity [72,256,[260][261][262][263]. [272] For instance, Janarthanan et al [273] fabricated PCL/α-TCP, gelatin/PCL/α-TCP, and fibronectin/PCL/α-TCP scaffolds using solvent casting method combined with gas foaming process. They showed that both gelatin/PCL/α-TCP and fibronectin/PCL/α-TCP scaffolds were characterized by superior biocompatibility in vitro compared to PCL/α-TCP biomaterial (studies on human ASCs).…”

Section: Bone Tementioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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“…Since GO also has good antibacterial properties [10,11], it has been used as surgical dressing to prevent infection and heal external wounds [12]. Moreover, a stable structure built by planting phospholipid bilayer on GO [13] can promote cell adhesion [14] and proliferation [15]. Many reports suggest that GO is biocompatible [16] and biostable [17] and hence serves as an interesting platform for biomedical applications [18,19].…”

Section: Introductionmentioning

confidence: 99%

Investigation on physicochemical properties of graphene oxide/nano-hydroxyapatite composites and its biomedical applications

Yang

Liu

et al. 2021

J Aust Ceram Soc

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Graphene oxide/nano-hydroxyapatite (GO/nHAP) composites were synthesized by simultaneous titration method. The GO powder was uniformly dispersed ultrasonically in a solution containing Ca(NO3)2. It was co-titrated with (NH4)2HPO4, during which NH3·H2O was used to maintain pH of about 10. Transmission electron microscopy (TEM) showed that HAP had a drusy acicular crystal structure with 100–200 nm length in the composite. The Ca2+ ions were attracted by the negatively charged oxygen functional groups present on GO sheets. They also oriented the growth of hydroxyapatite preferentially along (112) plane, which was also consistent with X-ray diffractometry (XRD) results. According to X-ray photoelectron spectroscopic (XPS) results, the peak intensities of the C–O and C–C groups increased in the GO/nHAP composite. However, the number of –COO– and C–O–C groups was reduced as well as the position of peaks shifted due to electrostatic interactions. These results were also corroborated with Fourier transform infrared spectroscopy (FTIR). MTT assay indicated that GO/nHAP composites had a significant effect on proliferation of 293T cells and good biomimetic mineralization as shown by in vitro bioactivity assays. EDS spectroscopy confirmed that the Ca/P ratio in calcium phosphate deposits was 1.62, which was close to the ratio of 1.64 in natural bone. The biological performance of GO/nHAP composite proved it to be a promising candidate for bone regeneration and implantation.

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Graphene oxide incorporated polycaprolactone/chitosan/collagen electrospun scaffold: Enhanced osteogenic properties for bone tissue engineering

Cited by 81 publications

References 73 publications

Preliminary In Vitro Evaluation of Chitosan–Graphene Oxide Scaffolds on Osteoblastic Adhesion, Proliferation, and Early Differentiation

Preliminary In Vitro Evaluation of Chitosan–Graphene Oxide Scaffolds on Osteoblastic Adhesion, Proliferation, and Early Differentiation

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Investigation on physicochemical properties of graphene oxide/nano-hydroxyapatite composites and its biomedical applications

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