Graphene oxide integrated sensor for electrochemical monitoring of mitomycin C–DNA interaction

Abstract

We present a graphene oxide (GO) integrated disposable electrochemical sensor for the enhanced detection of nucleic acids and the sensitive monitoring of the surface-confined interactions between the anticancer drug mitomycin C (MC) and DNA. Interfacial interactions between immobilized calf thymus double-stranded (dsDNA) and anticancer drug MC were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Based on three repetitive voltammetric measurements of 120 μg mL(-1) DNA immobilized on GO-modified electrodes, the RSD % (n = 3) was calculated as 10.47% and the detection limit (DL) for dsDNA was found to be 9.06 μg mL(-1). EIS studies revealed that the binding of the drug MC to dsDNA leads to a gradual decrease of its negative charge. As a consequence of this interaction, the negative redox species were allowed to approach the electrode, and thus increase the charge transfer kinetics. On the other hand, DPV studies exploited the decrease of the guanine signal due to drug binding as the basis for specifically probing the biointeraction process between MC and dsDNA.

Electrochemical monitoring of the interaction between mitomycin C and DNA at chitosan--carbon nanotube composite modified electrodes

“…MC is an alkylating agent and this alkylation process is related to the sequence specific to guanine nucleoside in the sequence 5'-CpG-3' (shown in Figure 6 in the scheme). Similar to the results in earlier reports, 6,17,23,24,27 it was aimed to monitor a decrease in guanine oxidation signal in the case of the interaction of MC with DNA.…”

A functional graphene oxide-ionic liquid composites–gold nanoparticle sensing platform for ultrasensitive electrochemical detection of Hg2+

“…When the bare electrode was modied with AuNPs, the peak currents increased and the peak-to-peak separation (DE p ) became smaller (Fig. 2c), indicating AuNPs were in favor of the CV of [Fe(CN) 6 ] 3À/4À . The largest peak current was obtained on the AuNPs-GO-IL-GCE (Fig.…”

“…Therefore, in the electrochemistry eld, GO as membrane material for modifying electrodes has shown great application potential. For example, GO-Au nanocluster composites modied glassy carbon electrode (GCE) is constructed for amplied voltammetric detection of L-cysteine, 5 GO integrated pencil graphite electrode is fabricated for electrochemical monitoring of mitomycin C-DNA interaction, 6 and Fe 3 O 4 -GO nanocomposite modied GCE is developed for amino acid sensing. 7 As seen, GO-based composite materials have received wide attention owing to the synergistic contribution of two or more functional components and many potential applications.…”

Synthesis of Cobalt hexacyanoferrate decorated graphene oxide/carbon nanotubes-COOH hybrid and their application for sensitive detection ofhydrazine

“…Its various oxygen-containing functional groups may protect carbon sheets from restacking and agglomeration, make it disperse in water and some other solvents readily [2,3], and also serve as the combining sites for graphene-based nanocomposites. But, its poor electrical conductivity limits its direct application as electrically active materials.…”