Graphene oxide-mediated fluorescence turn-on GO-FAM-FRET aptasensor for detection of sterigmatocystin

Gade, Pravin Savata; Sonkar, Rutuja Murlidhar; Bhatt, Praveena

doi:10.1039/d2ay01405j

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…The GO used in this study was synthesized and characterized in our previous work. , The hydrolysate was treated with different concentrations of GO (1–5 mg/mL). The treated hydrolysate was incubated at 200 rpm for 1 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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Green Downstream Processing Method for Xylooligosaccharide Purification and Assessment of Its Prebiotic Properties

Sonkar,

Gade,

Mudliar

et al. 2023

ACS Omega

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Xylooligosaccharides (XOS) obtained from lignocellulosic biomass after autohydrolysis primarily consist of ligninderived impurities and autogenerated inhibitors like furfural, hydroxymethylfurfural, and acetic acid. In this study, graphene oxide-mediated purification (GOMP), a novel and environmentally friendly downstream processing method, was developed for the purification of XOS from hydrolysate obtained after ozone-assisted autohydrolysis of wheat bran. GOMP resulted in appreciable recovery of total XOS from the hydrolysate (73.87 ± 4.25%, DP2− 6) with near complete removal of autogenerated inhibitors (furfural 85.42%, HMF 87.38%, and acetic acid 84.0%). Recovery of XOS by GOMP was higher than the conventional membrane purification technique (44.07 ± 0.92%) and activated charcoal treatment (72.76 ± 0.84%) along with comparatively higher removal of inhibitor compounds. GOMP results in the selective adsorption of inhibitors on the graphene oxide matrix from the XOS-rich hydrolysate, resulting in its purification and concentration. The prebiotic function of the obtained XOS fractions (DP2−4.48%, DP3−39.69%, DP4−36.13%, DP5−8.38%, and DP6−13.10%) was evaluated, indicating the growth stimulation of tested probiotic cultures and differential utilization of XOS oligomers DP3 and DP4 and complete consumption of DP2, DP5, and DP6 along with short-chain fatty acids as a major fermentation product. These findings suggest that GOMP, which employs a common substance (i.e., graphene oxide) used in water treatment, exhibits potential as an efficient and economically viable single-step methodology for XOS purification.

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Section: Methodsmentioning

confidence: 99%

“…GO is an emergent catalyst and has been used extensively as an adsorbent for the removal of water contaminants (bacteria, heavy metals, mercury, fluoride, copper, and arsenic) . It has a negatively charged large surface area, thermal stability, mechanical strength, excellent conductivity, and metal-free catalyst. − …”

Section: Introductionmentioning

confidence: 99%

Green Downstream Processing Method for Xylooligosaccharide Purification and Assessment of Its Prebiotic Properties

Sonkar,

Gade,

Mudliar

et al. 2023

ACS Omega

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“…Python was used to simulate the drug release model (forward problem) while GA was used to solve the inverse problem. In GA, body weight was set to 70 and dose to 500 [32].…”

Section: Simulation; In Silico Modellingmentioning

confidence: 99%

Design, Characterization and in vitro Simulations of nano-HAP/GO Composite Drug Delivery System Produced by Hydrothermal Methods Loaded with Paclitaxel

Çiftçi

2023

Cumhuriyet Science Journal

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In this study, it was aimed to develop a nano drug system that can be used in passive targeting in pancreatic cancer treatment. Hydroxyapatite nanocrystals (n-HAP) produced by hydrothermal process and graphene oxide (GO) produced by hummers method were used to increase the carrier capacity of the nano drug system and to activate the drug release kinetics and drug loading capacity. Analyses performed for nanocomposite drug carrier systems; FT-IR, XRD, TGA, BET analysis, Zeta potential, TEM and SEM. Paclitaxel (PTX), a chemotherapeutic drug used in the treatment of pancreatic cancer, was loaded into HAP nanocrystals (PTX- loaded n-HAP) and its activity on pancreatic cancer cells was investigated. When PTX was 1 and 2 mg, Encapsulation Efficiency (EE) and Drug Loading Content (LC) were 79.17-72.24% and 80.01-80.27%, respectively, for H-n-HAP crystal structure only, while EE and LC were 88.57-81.57% and 90.84-110.57%, respectively, when H-n-HAP crystal structure was loaded with 1 and 2 mg PTX together with GO. Here, it was observed PTX release profiles are according to the Hixson model. According to Fick's law, release profile was observed with values of k=1.89, n=0.21, SSD=0.04, R2=0.997, FIC=2.03, SD=0.004. In cell culture studies, as GO nanomaterials were loaded into H-n-HAP nanocrystal structure, the effect of PTX drug on pancreatic cancer increased and the viability of cancer cells decreased. It can be concluded that H-n-HAP/GO/PTX nanocomposite structure kills more pancreatic cancer cells with synergistic effect.

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Process, advances, and perspectives of graphene oxide-SELEX for the development of aptamer molecular probes: A comprehensive review

Chen,

Yang,

Cui

et al. 2024

Analytica Chimica Acta

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Graphene oxide-mediated fluorescence turn-on GO-FAM-FRET aptasensor for detection of sterigmatocystin

Abstract: A simple one-step graphene oxide mediated fluorescence turn-on GO-FAM-FRET aptasensor for detecting STC in food and feed matrices.

Cited by 4 publications

References 27 publications

Green Downstream Processing Method for Xylooligosaccharide Purification and Assessment of Its Prebiotic Properties

Green Downstream Processing Method for Xylooligosaccharide Purification and Assessment of Its Prebiotic Properties

Design, Characterization and in vitro Simulations of nano-HAP/GO Composite Drug Delivery System Produced by Hydrothermal Methods Loaded with Paclitaxel

Process, advances, and perspectives of graphene oxide-SELEX for the development of aptamer molecular probes: A comprehensive review

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