Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death

Krunic, Matija; Ristić, Biljana; Bošnjak, Mihajlo; Paunović, Verica; Zogović, Nevena; Mirčić, Aleksandar; Marković, Zoran M.; Marković, Biljana M. Todorović; Jovanović, Svetlana P.; Kleut, Duška; Mojović, Miloš; Nakarada, Đura; Marković, Olivera; Vuković, Irena; Harhaji-Trajković, Ljubica; Trajkovič, Vladimir

doi:10.1016/j.freeradbiomed.2021.10.025

Cited by 13 publications

(14 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 53 In another study with graphene quantum dots, they have been shown to have a protective effect on SHSY5Y cells. 54 However, in these studies, no relationship was established with the surface area of graphene.…”

Section: Resultsmentioning

confidence: 94%

“…In studies using SH-SY5Y cells, it has been shown that GO does not induce apoptosis and has no significant cytotoxicity at low concentrations (<80 μg/mL), but the viability of cells exhibits dose and time-dependent decreses at high concentrations (≥80 μg/mL) . In another study with graphene quantum dots, they have been shown to have a protective effect on SHSY5Y cells . However, in these studies, no relationship was established with the surface area of graphene.…”

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Surface Area of Graphene Governs Its Neurotoxicity

Taşdemir

Morçimen

Dogan

et al. 2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Due to their unique physicochemical properties, graphene and its derivatives are widely exploited for biomedical applications. It has been shown that graphene may exert different degrees of toxicity in in vivo or in vitro models when administered via different routes and penetrated through physiological barriers, subsequently being distributed within tissues or located within cells. In this study, in vitro neurotoxicity of graphene with different surface areas (150 and 750 m 2 /g) was examined on dopaminergic neuron model cells. SH-SY5Y cells were treated with graphene possessing two different surface areas (150 and 750 m 2 /g) in different concentrations between 400 and 3.125 μg/mL, and the cytotoxic and genotoxic effects were investigated. Both sizes of graphene have shown increased cell viability in decreasing concentrations. Cell damage increased with higher surface area. Lactate dehydrogenase (LDH) results have concluded that the viability loss of the cells is not through membrane damage. Neither of the two graphene types showed damage through lipid peroxidation (MDA) oxidative stress pathway. Glutathione (GSH) values increased within the first 24 and 48 h for both types of graphene. This increase suggests that graphene has an antioxidant effect on the SH-SY5Y model neurons. Comet analysis shows that graphene does not show genotoxicity on either surface area. Although there are many studies on graphene and its derivatives on their use with different cells in the literature, there are conflicting results in these studies, and most of the literature is focused on graphene oxide. Among these studies, no study examining the effect of graphene surface areas on the cell was found. Our study contributes to the literature in terms of examining the cytotoxic and genotoxic behavior of graphene with different surface areas.

show abstract

Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 97%

Surface Area of Graphene Governs Its Neurotoxicity

Taşdemir

Morçimen

Dogan

et al. 2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…35 Krunić et al explored the anti-oxidative property of GQDs against oxidative and nitrative damages in SH-SY5Y neuroblastoma cells generated through sodium nitroprusside, with GQDs acting as non-selective antioxidants and iron chelators, with no enzymatic activity. 36 More interestingly, Wang et al showed that upon halogen doping, GQDs shows a switch between its anti- and pro-oxidant behavior with good antibacterial effect mediated through generation ( 1 O 2 ). 37 Although multiple attempts are made to use GQDs-like enzyme mimics to treat various diseases/disorders associated with stress, its exact treatment mechanism and administrative dose for human applications is yet to be resolved.…”

Section: Introductionmentioning

confidence: 99%

Coal waste-derived synthesis of yellow oxidized graphene quantum dots with highly specific superoxide dismutase activity: characterization, kinetics, and biological studies

Das,

Kumar

et al. 2023

Nanoscale

View full text Add to dashboard Cite

show abstract

“…Increases in reactive oxygen species (ROS) can lead to protein oxidation, DNA damage, alteration of ion channels, and disruption of mitochondrial membrane potential, ultimately resulting in neuronal cell death (1). The brain is particularly vulnerable to oxidative stress because it consumes large amounts of oxygen, contains high levels of fatty acids that are susceptible to peroxidation, and has a weak antioxidant capacity (2). The development of ROS has been associated with neurodegenerative diseases such as Parkinson's disease (PD) (2,3).…”

Section: Introductionmentioning

confidence: 99%

Resveratrol Karbon Noktalarının SH-SY5Y İnsan Nöroblastoma Hücrelerinde 6-OHDA Kaynaklı Nöronal Hücre Ölümü Üzerinde Koruyucu Etkileri

CİCEK,

DANIŞMAN

2023

Harran Üniversitesi Tıp Fakültesi Dergisi

View full text Add to dashboard Cite

Background: We aimed to investigate the ability of resveratrol carbon dots (RES C-Dots) to protect SH-SY5Y cells from oxido-inflammatory stress and apoptosis caused by 6-hydroxydopamine (6-OHDA). Materials and Methods: In vitro PD model was generated in SH-SY5Y cells by administering of 200 µM 6-OHDA for 24 hours. Different concentrations of RES C-Dots (12.5, 25, and 50 µg/mL) were applied to the cells 30 minutes before administration of 6-OHDA. Results: We observed that application of RES C-Dots prevented cell death induced by 6-OHDA and main-tained cell viability. As expected, RES C-Dots prevented oxidative damage induced by 6-OHDA - by strengthening the total amount of antioxidants and lowering the total amount of oxidants in SH-SY5Y cells. Similarly, RES C-Dots markedly alleviated the secretion of inflammatory factors (TNF-α and IL-1β) promot-ed by 6-OHDA. Furthermore, RES C-Dots prevented apoptosis induced by 6-OHDA by suppressing caspase-3 mRNA expression level. Conclusions: RES C-Dots rescued SH-SY5Y cells from 6-OHDA- induced damage by modulating the oxido-inflammatory and apoptotic response. This report indicates enounces that RES- synthesised C-Dots may have promising curative potential for PD.

show abstract

Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death

Cited by 13 publications

References 80 publications

Surface Area of Graphene Governs Its Neurotoxicity

Surface Area of Graphene Governs Its Neurotoxicity

Coal waste-derived synthesis of yellow oxidized graphene quantum dots with highly specific superoxide dismutase activity: characterization, kinetics, and biological studies

Resveratrol Karbon Noktalarının SH-SY5Y İnsan Nöroblastoma Hücrelerinde 6-OHDA Kaynaklı Nöronal Hücre Ölümü Üzerinde Koruyucu Etkileri

Contact Info

Product

Resources

About