Graphene Quantum Dots and Enzyme-Coupled Biosensor for Highly Sensitive Determination of Hydrogen Peroxide and Glucose

Wang, Bingdi; Shen, Jing; Huang, Yanjun; Liu, Zhenning; Zhuang, Hong

doi:10.3390/ijms19061696

Cited by 29 publications

(17 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A GQD-biosensor based on the synergetic effect of enzyme-coupled technique and fluorescence quenching was engineered for monitoring glucose levels in human serum samples [114]. As explained in Figure 4, oxidation of glucose was achieved by the glucose oxidase (GOx) enzyme, producing H 2 O 2 .…”

Section: Fluorescence-based Gqd Sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

190

View full text Add to dashboard Cite

Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

show abstract

Section: Fluorescence-based Gqd Sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

190

View full text Add to dashboard Cite

show abstract

“…In vivo tests carried out using human serum showed good selectivity when faced with interfering molecules, and the good reproducibility and the ease with which molecules immobilize onto the sensor’s surface gives the potential for development towards other biosensor types. Our next device employs a fluorescence quenching mechanism in the detection of glucose, using a combination of enzymatic reactions and graphene quantum dots (GQDs) to successfully create a linear relationship between glucose concentration in serum and the fluorescent intensity of the enzymatic reactions over a range of 0.2–10 μM, using enzymatic coupling of GOD and horseradish peroxidase ( Wang et al, 2018 ). High sensitivity was achieved due to the optical properties of the GQDs, as well as high selectivity when faced with other molecules due to the enzyme coupling method.…”

Section: Continuous Monitoring Of Glucose In Different Body Fluidsmentioning

confidence: 99%

Advances in Biosensors for Continuous Glucose Monitoring Towards Wearables

Johnston

Wang

et al. 2021

Front. Bioeng. Biotechnol.

104

View full text Add to dashboard Cite

Continuous glucose monitors (CGMs) for the non-invasive monitoring of diabetes are constantly being developed and improved. Although there are multiple biosensing platforms for monitoring glucose available on the market, there is still a strong need to enhance their precision, repeatability, wearability, and accessibility to end-users. Biosensing technologies are being increasingly explored that use different bodily fluids such as sweat and tear fluid, etc., that can be calibrated to and therefore used to measure blood glucose concentrations accurately. To improve the wearability of these devices, exploring different fluids as testing mediums is essential and opens the door to various implants and wearables that in turn have the potential to be less inhibiting to the wearer. Recent developments have surfaced in the form of contact lenses or mouthguards for instance. Challenges still present themselves in the form of sensitivity, especially at very high or low glucose concentrations, which is critical for a diabetic person to monitor. This review summarises advances in wearable glucose biosensors over the past 5 years, comparing the different types as well as the fluid they use to detect glucose, including the CGMs currently available on the market. Perspectives on the development of wearables for glucose biosensing are discussed.

show abstract

“…Moreover, this sensor platform combined with glucose oxidase (GOx) was further extended to the glucose sensing in human urine. The probe showed satisfactory recoveries of 94.6%−98.8% (Wang, Shen, et al, 2018). A dual enzyme-coupled and fluorescence quenching mechanism-based detection strategy for glucose was developed using highly fluorescent GQDs.…”

Section: 76mentioning

confidence: 99%

Recent progress on graphene quantum dots‐based fluorescence sensors for food safety and quality assessment applications

Sharma

Ali

Devaraj

et al. 2021

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

The versatile photophysicalproperties, high surface-to-volume ratio, superior photostability, higher biocompatibility, and availability of active sites make graphene quantum dots (GQDs) an ideal candidate for applications in sensing, bioimaging, photocatalysis, energy storage, and flexible electronics. GQDsbased sensors involve luminescence sensors, electrochemical sensors, optical biosensors, electrochemical biosensors, and photoelectrochemical biosensors.Although plenty of sensing strategies have been developed using GQDs for biosensing and environmental applications, the use of GQDs-based fluorescence techniques remains unexplored or underutilized in the field of food science and technology. To the best of our knowledge, comprehensive review of the GQDsbased fluorescence sensing applications concerning food quality analysis has not yet been done. This review article focuses on the recent progress on the synthesis strategies, electronic properties, and fluorescence mechanisms of GQDs.The various GQDs-based fluorescence detection strategies involving Förster resonance energy transfer-or inner filter effect-driven fluorescence turn-on and turn-off response mechanisms toward trace-level detection of toxic metal ions, toxic adulterants, and banned chemical substances in foodstuffs are summarized. The challenges associated with the pretreatment steps of complex food matrices and prospects and challenges associated with the GQDs-based fluorescent probes are discussed. This review could serve as a precedent for further advancement in interdisciplinary research involving the development of versatile GQDs-based fluorescent probes toward food science and technology applications.

show abstract

Graphene Quantum Dots and Enzyme-Coupled Biosensor for Highly Sensitive Determination of Hydrogen Peroxide and Glucose

Cited by 29 publications

References 55 publications

Applications of Graphene Quantum Dots in Biomedical Sensors

Applications of Graphene Quantum Dots in Biomedical Sensors

Advances in Biosensors for Continuous Glucose Monitoring Towards Wearables

Recent progress on graphene quantum dots‐based fluorescence sensors for food safety and quality assessment applications

Contact Info

Product

Resources

About