Graphene‐Supported Hemin as a Highly Active Biomimetic Oxidation Catalyst

Tian, Xue; Jiang, Shan; Qu, Yongquan; Su, Qiao; Cheng, Rui; Dubin, Sergey; Chiu, Chin‐Yi; Kaner, Richard B.; Huang, Yu; Duan, Xiangfeng

doi:10.1002/anie.201108400

Cited by 326 publications

(261 citation statements)

References 40 publications

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“…As we know, K m represents the affinity of an enzyme toward substrates. A higher affinity for the substrate is reflected by smaller K m and vice versa [37]. In our study, the obtained K m and V max values of NiS/MMT/GO were comparatively investigated with that of HRP and other peroxidase analogues, listed in NiS/MMT/GO was slightly higher than that of MMT [31] and GO-COOH [42], suggesting the relatively weak affinity between TMB and the NiS/MMT/GO composites than them.…”

Section: Steady-state Kinetic Study Of Nis/mmt/go Nanocompositesmentioning

confidence: 79%

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Chen

Zhu

Yang

et al. 2018

Adv Compos Hybrid Mater

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In the present study, a facile method was proposed to prepare nickel sulfide (NiS) nanoparticles well-dispersed on the surface of montmorillonite/graphene oxide (MMT/GO). The NiS/MMT/GO nanocomposites were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), fluorescence spectrophotometry, etc. Significantly, the novel NiS/MMT/GO nanocomposites exhibited high peroxidase-like activity toward the typical chromogenic substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). However, glutathione (GSH) could inhibit the peroxidase-like activity of NiS/ MMT/GO along with a visible color variation. Based on the color change of the system of nanocomposites-H 2 O 2 -TMB, a novel colorimetric sensor was designed and conveniently used to quantitatively detect H 2 O 2 as well as GSH. The limit of detection (LOD) of H 2 O 2 and GSH is 9.73 and 0.5043 μM, respectively. The proposed H 2 O 2 sensor has potential application in the field of biosensing, food, and environment.

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Section: Steady-state Kinetic Study Of Nis/mmt/go Nanocompositesmentioning

confidence: 79%

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Chen

Zhu

Yang

et al. 2018

Adv Compos Hybrid Mater

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“…The graphene FET was non-covalently functionalized with hemin chloride through the p-p stacking interaction to form an NO sensor (Fig. 1b) 35 . Hemin is chosen as the functionalization molecule because of its large binding constant and high selectivity towards NO 36 .…”

Section: Fabrication and Characterization Of Graphene-hemin Sensorsmentioning

confidence: 99%

Real-time electrical detection of nitric oxide in biological systems with sub-nanomolar sensitivity

et al. 2013

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Real-time monitoring of nitric oxide concentrations is of central importance for probing the diverse roles of nitric oxide in neurotransmission, cardiovascular systems and immune responses. Here we report a new design of nitric oxide sensors based on hemin-functionalized graphene field-effect transistors. With its single atom thickness and the highest carrier mobility among all materials, graphene holds the promise for unprecedented sensitivity for molecular sensing. The non-covalent functionalization through p-p stacking interaction allows reliable immobilization of hemin molecules on graphene without damaging the graphene lattice to ensure the highly sensitive and specific detection of nitric oxide. Our studies demonstrate that the graphene-hemin sensors can respond rapidly to nitric oxide in physiological environments with a sub-nanomolar sensitivity. Furthermore, in vitro studies show that the graphene-hemin sensors can be used for the detection of nitric oxide released from macrophage cells and endothelial cells, demonstrating their practical functionality in complex biological systems.

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“…Moreover, graphene oxide also possesses a rich surface chemistry and has the potential to further promote the catalytic activity and stability of the supported molecular systems such as hemin and other porphyrin species through cation-π interactions or π-π stacking [19]. Hunag et al [20] prepared a GO-haemoglobin composite supramolecular hydrogel by directly mixing the dispersions of both components and explored its application as a catalyst for a peroxidatic reaction in organic solvents.…”

Section: Covalent Interactionmentioning

confidence: 99%

Interaction of Graphene Oxide with Proteins and Applications of their Conjugates

2017

JNMR

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Graphene oxide (GO) has abundant surface oxygen-containing groups such as epoxide, hydroxyl, and carboxylic groups; it can be prepared through the oxidative intercalation and exfoliation of graphite on a mass scale. Owing to the enriched surface functionalities, the GO is water-soluble and chemically versatile. The surface functional groups can also provide plenty of reaction sites for linking nanoparticles, proteins, enzymes, peptides, bacteria, cells, and nucleic acids through covalent and non-covalent binding. GO has been used as a matrix for protein immobilization in different biotechnological applications such as fluorescence-or electrochemical-based sensors, labeling and imaging, therapy, and targeted delivery. This paper reviews the main strategies for the assembly of proteins onto graphene oxide surface and their applications, especially in the biomedical area.

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Graphene‐Supported Hemin as a Highly Active Biomimetic Oxidation Catalyst

Cited by 326 publications

References 40 publications

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Real-time electrical detection of nitric oxide in biological systems with sub-nanomolar sensitivity

Interaction of Graphene Oxide with Proteins and Applications of their Conjugates

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