The purpose of this study was to investigate the graph interpretation ability and perception of high school students and preservice secondary teachers in Earth science. We developed two different instruments; one was a graph interpretation ability inventory that consists of 9 graph types with 18 items, and the other one is two questionnaires to explore the participants' perception about Earth science-related graph. The results of this study are as follows: High school students and preservice secondary teachers demonstrated their remarkable ability in interpreting a line graph, but showed their limited ability with the graph of overlapped and directional change, which means the graph interpretation ability was affected by a graph type; two groups participated in this study revealed a considerable difference in the graph interpretation ability depending on the grade level; preservice teachers were superior to high school students in discriminating two graphs, the representation method, which are different with the same topic; and many participants in both groups considered that the property of Earth science graph was considerably different from that of other science subjects, especially in directional change graph, scatter graph, contour map, and domain graph. The results suggest that the effective graph instruction strategies be developed in Earth science learning.