Graphs Defined on Rings: A Review

Madhumitha, S.; Naduvath, Sudev

doi:10.20944/preprints202308.0090.v1

Cited by 1 publication

(2 citation statements)

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“…Also, a YR4 amplified one such polymorphic fragment of 456bp while a CYR1 amplified another polymorphic fragment of 1236bp, present in tolerant lines, but absent in the susceptible cultivar [19]. Similar results are also reported primers RGA1 (RGA1FCG & 1R) and RGA8 (RGA8F & 8R) produced amplicon at 490-513bp and 387-390bp respectively [31]. Further, four RGA makers pairs showed the presence of consistent bands of size ~450bp in all resistant lines while absent in susceptible GM 4 [32].…”

Section: Screening Of Rga Markers Associated With Resistance Genesupporting

confidence: 84%

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Resistant Gene Analogue Marker(S) Screening against Yellow Mosaic Disease in Mungbean [Vigna radiata (L.) Wilczek]

Kalaria,

Bhabhor,

Modha

2023

IJPSS

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During Rabi 2021–2022, 100 genotypes of mungbean were tested for resistance to yellow mosaic disease in the field using the disease grading system. Out of the 100 mungbean genotypes, 49 were resistant to the yellow mosaic disease, 22 as Moderately resistant, 11 as Moderately susceptible, 3 as susceptible, and 15 were highly susceptible. The sequenced virus shares 98.94% similarity with the whole coding sequence of the MYMIV-Mb02 coat protein (AV1) gene from the mungbean yellow mosaic India virus strain, accession number GQ387502.1., as per the results of the BLAST program. As a result, the variation was designated mungbean yellow mosaic India virus isolates NAU-RJ coat protein gene segment and assigned the accession number ON622515.1. The current study examined Six resistant (40 C, NMS-21-01, NMS-21-06, NMS-21-22, NMS-21-49, NMS-21-95) and six highly susceptible (GM 4, NMS-21-23, NMS-21-24, NMS-21-40, NMS-21-68, NMS-21-69). In order to identify the six susceptible and six resistant mungbean genotypes, nine pairs of RGA primers were employed. Five pairs of RGA primers were successfully amplified in all resistant genotypes as a consequence, but not in all highly susceptible genotypes. The results of this study suggest that five pairs of RGA markers can effectively distinguish between the genotypes of mungbean that are highly susceptible and resistant. The long-lasting YMV resistance of these RGA markers makes them useful for mapping resistance genes and marker validation studies.

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Section: Screening Of Rga Markers Associated With Resistance Genesupporting

confidence: 84%

“…The phylogenetic tree built using the partial coat protein genes of mungbean yellow mosaic India virus isolate NAU-RJ coat protein gene segment DNA A showed two main clusters (Fig. 6 [30], in mungbean against MYMV [31]. Also, similar work carried out in mungbean against MYMV [32].…”

Section: Phylogenetic Analysis Of Cp Gene Of Dna-a Mymiv With Other B...mentioning

confidence: 82%