Grass Carp Reovirus Induces Formation of Lipid Droplets as Sites for Its Replication and Assembly

He, Libo; Wang, Qian; Li, Xinyu; Wang, Hanyue; Chu, Pengfei; Cheng, Yang; Li, Yongming; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping

doi:10.1128/mbio.02297-22

Cited by 9 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Viral infection promoted the expression of IDH1/2, which facilitates viral replication, and lipid synthesis is crucial for the maturation of viral particles [48]. Notably, GCRV-II displayed an additional abundance of differentially expressed genes involved in lipid biosynthesis and metabolic processes [49]. Ultrastructural analysis of GCRV-II-infected CIK cells revealed the presence of an extra lipid membrane surrounding viral particles [50].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Kong,

Ding,

Yang

et al. 2023

IJMS

View full text Add to dashboard Cite

Grass carp reovirus (GCRV), one of the most serious pathogens threatening grass carp (Ctenopharyngodon idella), can lead to grass carp hemorrhagic disease (GCHD). Currently, GCRV can be divided into three genotypes, but the comparison of their pathogenic mechanisms and the host responses remain unclear. In this study, we utilized the Ctenopharyngodon idella kidney (CIK) model infected with GCRV to conduct comparative studies on the three genotypes. We observed a cytopathic effect (CPE) in the GCRV-I and GCRV-III groups, whereas the GCRV-II group did not show any CPE. Moreover, a consistent trend in the mRNA expression levels of antiviral-related genes across all experimental groups of CIK cells was detected via qPCR and further explored through RNA-seq analysis. Importantly, GO/KEGG enrichment analysis showed that GCRV-I, -II, and -III could all activate the immune response in CIK cells, but GCRV-II induced more intense immune responses. Intriguingly, transcriptomic analysis revealed a widespread down-regulation of metabolism processes such as steroid biosynthesis, butanoate metabolism, and N-Glycan biosynthesis in infected CIK cells. Overall, our results reveal the CIK cells showed unique responses in immunity and metabolism in the three genotypes of GCRV infection. These results provide a theoretical basis for understanding the pathogenesis and prevention and control methods of GCRV.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Kong,

Ding,

Yang

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Knockdown of ARF1 by siRNA or inhibition of GTPase activity of ARF1 restricted the formation of VIBs 119 . Moreover, the host lipid metabolism was hijacked by GCRV to increase de novo fatty acid synthesis and accumulate lipid droplets, which were indispensable for the formation of VIBs 121 . HSP90 was a chaperone for VP35 protein and promoted efficient replication of GCRV‐II 117 .…”

Section: The Replication and Infection Of Aqrvsmentioning

confidence: 99%

Structure, function and immune evasion strategies of aquareoviruses, with focus on grass carp reovirus

Zhang

Chang

2023

Reviews in Aquaculture

View full text Add to dashboard Cite

Aquareoviruses (AqRVs) can infect various wild or cultured bony fishes with serious muscle and viscera haemorrhage syndrome and have caused severe economic losses worldwide. Accumulating evidences have revealed the functions of viral proteins of AqRVs in RNA transcription, virion assembly, cell attachment and/or viral replication. In addition, structural information and transcription mechanism of AqRVs have been in‐depth studied through cryo‐electron microscopy combined with three‐dimensional image reconstruction. Although far from enough, structural and functional studies of AqRVs provide substantial insights into how viral proteins coordinate the replication of AqRVs and escape the host immune response. Grass carp reovirus (GCRV) is the most pathogenic AqRV isolated from aquatic animals. The completion of GCRV lifecycle requires the participation of host proteins. So far, many host factors involved in the entry and replication of GCRV have been identified. Based on the function of viral proteins and the viral lifecycle, many vaccines and antiviral agents have been developed to prevent the replication and infection of GCRV. This review summarises the structural characteristics and the composition of core proteins of AqRVs, as well as synergistic mechanisms of structural proteins on genome transcription and virus entry of AqRVs. In addition, the review highlights our current knowledge for the infection and replication, functions of non‐structural proteins, immune escape mechanisms, as well as the vaccines and antiviral agents of AqRVs, especially for GCRV. A better understanding of structure, function and immune evasion strategies of AqRVs will offer new targets for prevention and control of AqRVs in aquaculture.

show abstract

Recombinant surface display vaccine enhances the immersion immune effect against grass carp reovirus in grass carp (Ctenopharyngodon idella)

Hao,

Wang,

Zhu

et al. 2023

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Grass Carp Reovirus Induces Formation of Lipid Droplets as Sites for Its Replication and Assembly

Abstract: Grass carp reovirus (GCRV) is the most virulent pathogen in the genus Aquareovirus , which belongs to the family Reoviridae . GCRV-induced hemorrhagic disease is a major threat to the grass carp aquaculture industry.

Cited by 9 publications

References 54 publications

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Structure, function and immune evasion strategies of aquareoviruses, with focus on grass carp reovirus

Recombinant surface display vaccine enhances the immersion immune effect against grass carp reovirus in grass carp (Ctenopharyngodon idella)

Contact Info

Product

Resources

About