Gravisensitivity of endothelial cells: the role of cytoskeleton and adhesion molecules

Rudimov, E. G.; Буравкова, Л. Б.

doi:10.1134/s0362119716060177

Cited by 11 publications

(18 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Investigations of the endothelium of blood vessels, which is involved in maintaining vessel integrity and function, are important to obtain a better understanding of cardiovascular changes that may occur in space. From this perspective, cultured endothelial cells are viewed as an adequate model for studying molecular mechanisms of gravireception …”

Section: Discussionmentioning

confidence: 99%

The ICAM‐1 expression level determines the susceptibility of human endothelial cells to simulated microgravity

Буравкова

Rudimov

Andreeva

et al. 2017

J of Cellular Biochemistry

View full text Add to dashboard Cite

Microgravity is a principal risk factor hampering human cardiovascular regulation during space flights. Endothelial dysfunction associated with the impaired integrity and uniformity of the monolayer represents a potential trigger for vascular damage. We characterized the expression profile of the multi-step cascade of adhesion molecules (ICAM-1, VCAM-1, E-selectin, VE-cadherin) in umbilical cord endothelial cells (ECs) after 24 h of exposure to simulated microgravity (SMG), pro-inflammatory cytokine TNF-α, and the combination of the two. Random Positioning Machine (RPM)-mediated SMG was used to mimic microgravity effects. SMG stimulated the expression of E-selectin, which is known to be involved in slowing leukocyte rolling. Primary ECs displayed heterogeneity with respect to the proportion of ICAM-1-positive cells. ECs were divided into two groups: pre-activated ECs displaying high proportion of ICAM-1 -cells (ECs-1) (greater than 50%) and non-activated ECs with low proportion of ICAM-1 -cells (ECs-2) (less than 25%). Only non-activated ECs-2 responded to SMG by elevating gene transcription and increasing ICAM-1 and VE-cadherin expression. This effect was enhanced after cumulative SMG-TNF-α exposure. ECs-1 displayed an unexpected decrease in number of E-selectin- and ICAM-1-positive ECs and pronounced up-regulation of VCAM1 upon activation of inflammation, which was partially abolished by SMG. Thus, non-activated ECs-2 are quite resistant to the impacts of microgravity and even exhibited an elevation of the VE-cadherin gene and protein expression, thus improving the integrity of the endothelial monolayer. Pre-activation of ECs with inflammatory stimuli may disturb the EC adhesion profile, attenuating its barrier function. These alterations may be among the mechanisms underlying cardiovascular dysregulation in real microgravity conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

The ICAM‐1 expression level determines the susceptibility of human endothelial cells to simulated microgravity

Буравкова

Rudimov

Andreeva

et al. 2017

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…The effects of gravity and microgravity on mammalian cells were known long before gravity effects on humans because cells can be exposed, relatively easily, to hypergravity by centrifugation and to simulated microgravity by using clinostats. Voluminous data on microgravity effects on cultured mammalian cells are now available from ground-based experiments using clinostats and also from experiments carried out on the International Space Station [4,5,6]. Some major changes noted in cultured mammalian cells when they are placed in a simulated [4,5,6] or true [7] microgravity environment include proliferation, motility, cytoskeletal reorganization, substrate adhesion, extracellular matrix synthesis, Ca 2+ homeostasis, gene and protein expressions, and cell signaling.…”

Section: Introductionmentioning

confidence: 99%

“…Voluminous data on microgravity effects on cultured mammalian cells are now available from ground-based experiments using clinostats and also from experiments carried out on the International Space Station [4,5,6]. Some major changes noted in cultured mammalian cells when they are placed in a simulated [4,5,6] or true [7] microgravity environment include proliferation, motility, cytoskeletal reorganization, substrate adhesion, extracellular matrix synthesis, Ca 2+ homeostasis, gene and protein expressions, and cell signaling. These changes are observed in many cell types, but the direction of changes (i.e., increase or decrease), such as for proliferation and motility, appears to depend on the cell type.…”

Section: Introductionmentioning

confidence: 99%

Increased Chromosome Aberrations in Cells Exposed Simultaneously to Simulated Microgravity and Radiation

Hada

Ikeda

Rhone

et al. 2018

IJMS

View full text Add to dashboard Cite

Space radiation and microgravity (μG) are two major environmental stressors for humans in space travel. One of the fundamental questions in space biology research is whether the combined effects of μG and exposure to cosmic radiation are interactive. While studies addressing this question have been carried out for half a century in space or using simulated μG on the ground, the reported results are ambiguous. For the assessment and management of human health risks in future Moon and Mars missions, it is necessary to obtain more basic data on the molecular and cellular responses to the combined effects of radiation and µG. Recently we incorporated a μG–irradiation system consisting of a 3D clinostat synchronized to a carbon-ion or X-ray irradiation system. Our new experimental setup allows us to avoid stopping clinostat rotation during irradiation, which was required in all other previous experiments. Using this system, human fibroblasts were exposed to X-rays or carbon ions under the simulated μG condition, and chromosomes were collected with the premature chromosome condensation method in the first mitosis. Chromosome aberrations (CA) were quantified by the 3-color fluorescent in situ hybridization (FISH) method. Cells exposed to irradiation under the simulated μG condition showed a higher frequency of both simple and complex types of CA compared to cells irradiated under the static condition by either X-rays or carbon ions.

show abstract

“…Space experiments and ground-based studies have demonstrated that ECs are very sensitive to modulation of a gravitational stimulus, demonstrating the signs of endothelial dysfunction 9–11 . Opportunities of studying the EC monolayer behavior under real microgravity are extremely limited; therefore, the data obtained in ground-based experiments are one of the most important sources of knowledge on the mechanisms of EC adaptation to microgravity.…”

Section: Introductionmentioning

confidence: 99%

“…To simulate the effects of microgravity on adherent cells, 2D- and 3D-clinorotation (random positioning machine (RPM)) are considered the most appropriate 12,13 . These models have shown that ECs demonstrate both early and delayed responses 9–11,14,15 . Exposure to adverse microenvironmental factors (such as inflammatory mediators) under microgravity may contribute to deterioration of the vascular wall condition.…”

Section: Introductionmentioning

confidence: 99%

Simulated microgravity modulates the mesenchymal stromal cell response to inflammatory stimulation

Ratushnyy

Yakubets

Andreeva

et al. 2019

Sci Rep

View full text Add to dashboard Cite

The duration and distance of manned space flights emphasizes the importance of advanced elucidation of space flight factors and their effects on human beings. The exposure to inflammatory mediators under microgravity may contribute to the activity of different cells, perivascular stromal cells (MSCs) in particular. Inflammatory activation is now considered as a principal cue of MSC engagement in reparative remodeling. In the present paper, the effect of simulated microgravity (sµg) on TNFα-mediated priming of adipose tissue-derived MSC (ASCs) was examined. Sµg per se did not induce inflammatory-related changes, such as elevation of ICAM-1 and HLA-ABC expression, soluble mediator production, or shifting of the transcription profile in ASCs. Moreover, the attenuated ASC response to TNFα priming under sµg was manifested in decreased production of TNFα-dependent pleiotropic cytokines (IL-8 and MCP-1), matrix remodeling proteases, and downregulation of some genes encoding growth factors and cytokines. Time-dependent analysis detected the first signs of priming attenuation after 48 hours of 3D-clinorotation. A reduced response of MSCs to priming under sµg can be a negative factor in terms of MSC involvement in tissue remodeling processes.

show abstract

Gravisensitivity of endothelial cells: the role of cytoskeleton and adhesion molecules

Cited by 11 publications

References 40 publications

The ICAM‐1 expression level determines the susceptibility of human endothelial cells to simulated microgravity

The ICAM‐1 expression level determines the susceptibility of human endothelial cells to simulated microgravity

Increased Chromosome Aberrations in Cells Exposed Simultaneously to Simulated Microgravity and Radiation

Simulated microgravity modulates the mesenchymal stromal cell response to inflammatory stimulation

Contact Info

Product

Resources

About