Greater Diversity of Shiga Toxin-Encoding Bacteriophage Insertion Sites among
            <i>Escherichia coli</i>
            O157:H7 Isolates from Cattle than in Those from Humans

Besser, Thomas E.; Shaikh, Nurmohammad; Holt, Nicholas J.; Tarr, Phillip I.; Konkel, Michael E.; Malik-Kale, Preeti; Walsh, Coilin W.; Whittam, Thomas S.; Bono, James L.

doi:10.1128/aem.02632-07

Cited by 11 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the strains studied here five different SBI genotypes were identified (1, 3, 5, 6, 21) ( Table 1) and genotype 1 (60·6%) was significantly more prevalent (P < 0·05) which may provide initial evidence of its epidemiological significance as an indicator of a potential infection risk from food sources in Egypt. This finding is consistent with former studies that described SBI genotype 1 as a potential risk genotype in the USA [4] and The Netherlands [26]. SBI 5 and 6 accounted for fewer strains (18·2% and 15·2%, respectively) while SBI 3 and 21 were infrequent (3%) suggesting the possible limited relevance of these genotypes in Egypt.…”

Section: Resultssupporting

confidence: 94%

“…Earlier studies have reported the importance of the molecular assays used in this study in identifying E. coli O157:H7 genotypes with variation in pathogenic potential and host specificity [3][4][5][6][7][8]. Furthermore, such assays were proposed as valuable indicators for countries with high rates of outbreak investigations with this organism [23].…”

Section: Resultsmentioning

confidence: 95%

“…Several epidemiological studies have characterized E. coli O157:H7 strains into genotypes associated with altered pathogenic potential and host specificity [3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multilocus genotypic characterization of Escherichia coli O157:H7 recovered from food sources

Elhadidy

Elkhatib

2014

Epidemiol. Infect.

View full text Add to dashboard Cite

Escherichia coli O157:H7 strains (n = 33) recovered from different food sources in Egypt were characterized using molecular assays to identify strain genotypes associated with various levels of pathogenic potential. Genotypic characterization included: lineage-specific polymorphism assay (LSPA-6), Shiga-toxin-encoding bacteriophage insertion site assay (SBI), clade 8 typing, Tir (A255 T) polymorphism, and variant analysis of Shiga toxin 2 gene (Stx 2a and Stx 2c), and anti-terminator Q genes (Q 933 and Q 21). Genotypes LI/II (76%), SBI 1 (60·6%), clade 8 (69·7%), Tir (255 T) (72·7%) and Stx 2c (45·5%) were found to be significantly more frequent compared to other genetic markers in the strains analysed. Multivariable analysis revealed a significant association between LPSA-6 and clade types as well as Tir (A255 T). To the best of our knowledge, this is the first study to report the characterization of these genetic markers in E. coli O157:H7 strains in the Middle East and Africa.

show abstract

Section: Resultssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

Multilocus genotypic characterization of Escherichia coli O157:H7 recovered from food sources

Elhadidy

Elkhatib

2014

Epidemiol. Infect.

View full text Add to dashboard Cite

show abstract

“…Evidence of at least two major O157:H7 lineages has been found in which lineage II was isolated more frequently from cattle than from humans (Kim et al, 1999;Zhang et al, 2007). Differences between those lineages were recently highlighted by us and others (Maldonado et al, 2005;Dowd and Ishizaki, 2006;Rashid et al, 2006;Besser et al, 2007;Lefebvre et al, 2008). Indeed, using DNA hybridization on arrays, we previously showed that O157:H7 isolates from beef cattle were genetically different from human isolates but possessed a similar virulence potential or, more precisely, a similar set of virulence genes (Lefebvre et al, 2008).…”

Section: Introductionmentioning

confidence: 89%

Relative Cytotoxicity ofEscherichia coliO157:H7 Isolates from Beef Cattle and Humans

Lefebvre

Diarra²,

Vincent³

et al. 2009

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

Differences and similarities between Escherichia coli O157:H7 isolates from beef cattle and those from sporadic human outbreaks are not fully elucidated. Here, we compared 44 O157:H7 isolates of bovine and human origins (22 isolates of each) to better understand their cytotoxic potential. The Shiga toxin genes stx1, stx2, or both were detected in the 44 isolates, and all elicited Vero cell cytotoxicity. The greatest cytotoxicity was caused by bovine isolates having only stx2 and which represented the majority of such isolates (81.8%). However, no correlation was found between the level of stx gene transcription and cytotoxicity. All human and bovine isolates possessed variant type stx2 and stx2c, respectively, as determined by PCR-restriction fragment length polymorphism. Isolates harboring both stx1 and stx2 genes were much more frequent in human isolates (86.4%). The combination stx1-stx2c found in only four bovine isolates was less cytotoxic. It is clear that cytotoxicity alone cannot account for the apparent inability of O157:H7 bovine isolates to cause diseases in humans. We have found that stx1-stx2-containing or stx1-stx2c-containing isolates were less cytotoxic than several bovine isolates having only stx2c, suggesting that the stx gene combination or other virulence genes in specific genetic lineages may affect the disease outcome.

show abstract

“…Some genotypes are highly associated with human clinical cases, while others are more frequently found in bovine cases (Besser et al, 2007). A recent study compared the expression patterns of clinical genotype 1 strains with those of bovine-biased genotype 5 strains using microarrays (Vanaja et al, 2010).…”

Section: Molecular Evolutionmentioning

confidence: 98%

Enterohemorrhagic Escherichia coli

Viazis

Diez-Gonzalez

2011

Advances in Agronomy

View full text Add to dashboard Cite

Greater Diversity of Shiga Toxin-Encoding Bacteriophage Insertion Sites among Escherichia coli O157:H7 Isolates from Cattle than in Those from Humans

Cited by 11 publications

References 0 publications

Multilocus genotypic characterization of Escherichia coli O157:H7 recovered from food sources

Multilocus genotypic characterization of Escherichia coli O157:H7 recovered from food sources

Relative Cytotoxicity ofEscherichia coliO157:H7 Isolates from Beef Cattle and Humans

Enterohemorrhagic Escherichia coli

Contact Info

Product

Resources

About