Greater Mouse-Eared Bats (Myotis myotis) Hibernating in the Nietoperek Bat Reserve (Poland) as a Vector of Airborne Culturable Fungi

Abstract

Bats can contribute to an increase of aeromycota in underground ecosystems and might be a vector/reservoir of microorganisms; however, there is no information about the number and species composition of fungi around hibernating bats. One of the most common species in Europe with direct human contact is the greater mouse-eared bat (Myotis myotis). The goal of our research was the first report of the airborne fungi present in the close vicinity of hibernating M. myotis in the Nietoperek bat reserve (Western Poland) by the use of culture-based techniques and genetic and phenotypic identifications. Aerobiological investigations of mycobiota under hibernating bats were performed on two culture media (PDA and YPG) and at two incubation temperatures (7 and 24 ± 0.5 °C). Overall, we detected 32 fungal species from three phyla (Ascomycota, Basidiomycota, and Zygomycota) and 12 genera. The application of YPG medium and the higher incubation temperature showed higher numbers of isolated fungal species and CFU. Penicillium spp. were dominant in the study, with spores found outside the underground hibernation site from 51.9% to 86.3% and from 56.7% to 100% inside the bat reserve. Penicillium chrysogenum was the most frequently isolated species, then Absidia glauca, Aspergillus fumigatus, A. tubingensis, Mortierella polycephala, Naganishia diffluens, and Rhodotorula mucilaginosa. Temperature, relative humidity, and the abundance of bats correlated positively with the concentration of airborne fungal propagules, between fungal species diversity, and the concentration of aeromycota, but the number of fungal species did not positively correlate with the number of bats. The air in the underground site was more contaminated by fungi than the air outside; however, the concentration of aeromycota does not pose a threat for human health. Nevertheless, hibernating bats contribute to an increase in the aeromycota and as a vector/reservoir of microscopic fungi, including those that may cause allergies and infections in mammals, and should be monitored.

Evidence of Antimicrobial Resistance in Bats and Its Planetary Health Impact for Surveillance of Zoonotic Spillover Events: A Scoping Review

“…Many of them were discovered near open spaces and vegetation along water bodies [37]. Interestingly, Myotis species have been discovered hibernating in caves with a wide range of microclimate conditions, whereas their breeding populations have primarily been established in buildings [37][38][39]. Another widespread species was the straw-colored fruit bat (Eidolon helvum), a huge Old World fruit bat (Pteropodidae) found across sub-Saharan Africa [40,41].…”

Gypsum Cave Biofilm Communities are Strongly Influenced by Bat- And Arthropod-Related Fungi

“…Another fungal group of interest in Covadura Cave is the class Tremellomycetes that includes the basidiomycetous yeasts Filobasidium, Apiotrichum, Vishniacozyma, Cutaneotrichosporon, and Dioszegia . These genera have been associated with bats [ 64 , 71 ]. In the C3 Cave only Vishniacozyma (= Cryptococcus ) was identified.…”

Keratinophilic and Keratinolytic Fungi in Cave Ecosystems: A Culture-Based Study of Brestovská Cave and Demänovská Ľadová and Slobody Caves (Slovakia)

“…More importantly, the number of species inside the studied caves was greater than those outside the underground sites. The species biodiversity of keratinophilic and keratinolytic fungi in the underground ecosystems, as in the case of fungi in the air, probably also depends on other factors, such as those of anthropogenic origin (e.g., the number of visitors, the period from which the object was made available to tourists) as well as the abundance of animals living in this type of site [6,[103][104][105]. It can also be assumed that the specific microclimate inside the…”