Greater public health impact of COVID-19 antigen detection tests

Kowada, Akiko

doi:10.1186/s12916-021-01956-z

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…Viral antigen tests have recently emerged as key diagnostic tools that can implement the global efforts of monitoring and containment of the COVID-19 pandemic. 57 With the aim of developing an antigen test for SARS-CoV-2 based on the spike glycoprotein, previously reported spike protein RBD domain-binding aptamers 46 were exploited. The binding affinities of all full-length aptamers (1–6) and the two truncated versions (1C and 4C) for both S1 and RBD proteins of SARS-CoV-2 were initially evaluated using direct ELAAs.…”

Section: Resultsmentioning

confidence: 99%

“…The lower homology of S proteins among the known HCoV’s compared to the N protein renders the S protein as the most appropriate antigen for the development of sensitive and specific antigen tests. Viral antigen tests have recently emerged as key diagnostic tools that can implement the global efforts of monitoring and containment of the COVID-19 pandemic . With the aim of developing an antigen test for SARS-CoV-2 based on the spike glycoprotein, previously reported spike protein RBD domain-binding aptamers were exploited.…”

Section: Resultsmentioning

confidence: 99%

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Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen

et al. 2021

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The novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) emerged at the end of 2019, resulting in the ongoing COVID-19 pandemic. The high transmissibility of the virus and the substantial number of asymptomatic individuals have led to an exponential rise in infections worldwide, urgently requiring global containment strategies. Reverse transcription-polymerase chain reaction is the gold standard for the detection of SARS-CoV-2 infections. Antigen tests, targeting the spike (S) or nucleocapsid (N) viral proteins, are considered as complementary tools. Despite their shortcomings in terms of sensitivity and specificity, antigen tests could be deployed for the detection of potentially contagious individuals with high viral loads. In this work, we sought to develop a sandwich aptamer-based assay for the detection of the S protein of SARS-CoV-2. A detailed study on the binding properties of aptamers to the receptor-binding domain of the S protein in search of aptamer pairs forming a sandwich is presented. Screening of aptamer pairs and optimization of assay conditions led to the development of a laboratory-based sandwich assay able to detect 21 ng/mL (270 pM) of the protein with negligible cross-reactivity with the other known human coronaviruses. The detection of 375 pg of the protein in viral transport medium demonstrates the compatibility of the assay with clinical specimens. Finally, successful detection of the S antigen in nasopharyngeal swab samples collected from suspected patients further establishes the suitability of the assay for screening purposes as a complementary tool to assist in the control of the pandemic.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen

et al. 2021

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“…Second, the antigen detection of SARS-COV-2 itself, without a window period, can be used for early screening of infection. However, studies have shown that its sensitivity and specificity are lower than those of nucleic acid and antibody tests, and it increases the probability of false positives and false negatives ( Kowada, 2021 ). In addition, the risk of infection in antigen detection is a problem that cannot be ignored, but it is ideal for point-of-care testing (POCT) because it can be performed with only nasopharyngeal swabs and LFIA kits, and if it is accompanied by relevant monitoring software, it can greatly save labor and material resources, control the spread of the epidemic, and obtain large data related to the epidemic, and it can facilitate self-testing, thus reducing psychological anxiety and worry.…”

Section: Lfia Technique For Sars-cov-2 Antigen Detectionmentioning

confidence: 99%

Rapid detection of SARS-CoV-2: The gradual boom of lateral flow immunoassay

Zhu

Zhou

et al. 2023

Front. Bioeng. Biotechnol.

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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is still in an epidemic situation, which poses a serious threat to the safety of people and property. Rapid diagnosis and isolation of infected individuals are one of the important methods to control virus transmission. Existing lateral flow immunoassay techniques have the advantages of rapid, sensitive, and easy operation, and some new options have emerged with the continuous development of nanotechnology. Such as lateral flow immunoassay test strips based on colorimetric-fluorescent dual-mode and gold nanoparticles, Surface Enhanced Raman Scattering, etc., these technologies have played an important role in the rapid diagnosis of COVID-19. In this paper, we summarize the current research progress of lateral flow immunoassay in the field of Severe Acute Respiratory Syndrome Coronavirus 2 infection diagnosis, analyze the performance of Severe Acute Respiratory Syndrome Coronavirus 2 lateral flow immunoassay products, review the advantages and limitations of different detection methods and markers, and then explore the competitive CRISPR-based nucleic acid chromatography detection method. This method combines the advantages of gene editing and lateral flow immunoassay and can achieve rapid and highly sensitive lateral flow immunoassay detection of target nucleic acids, which is expected to be the most representative method for community and clinical point-of-care testing. We hope that researchers will be inspired by this review and strive to solve the problems in the design of highly sensitive targets, the selection of detection methods, and the enhancement of CRISPR technology, to truly achieve rapid, sensitive, convenient, and specific detection of novel coronaviruses, thus promoting the development of novel coronavirus diagnosis and contributing our modest contribution to the world’s fight against epidemics.

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“…Tek kullanımlık, 15-20 dakika gibi kısa sürede hasta başında sonuç verebilen ve büyük ölçekli dağıtımı mümkün olan görece ucuz testlerdir. Özgül olmakla birlikte, duyarlılığı (özellikle hastalığın erken döneminde) düşüktür 1,10,25 .…”

Section: Hızlı Antijen Testleriunclassified

Usage of Diagnosis and Screening Tests in New Coronavirus Disease (Covıd-19)

BULDUK,

ÇÖL

2023

Arşiv Kaynak Tarama Dergisi

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COVID-19 ile mücadelede; hastalıkla enfekte olmuş kişilerin tespiti ve sonrasında izole edilmesi son derece önemlidir. Bu amaçla dünya genelinde kullanılan tanı testleri, çeşitlilik ve uygulama kriterleri açısından farklılık göstermektedir. Bu derlemede halk sağlığı bakış açısıyla; COVID-19 tanısı için kullanılan testler ile kimlere yönelik ve nasıl kullanıldığının yanısıra test kullanımı konusundaki yaklaşımlar ele alınmıştır. Polimeraz zincir reaksiyonu (PCR) tabanlı testler, yüksek duyarlılık ve özgüllük değerleri dolayısıyla altın standart olarak kabul edilmektedir. Farklı örnek alma yöntemlerine yönelik karşılaştırmalar yapılmakla birlikte genel görüş, nazofaringeal sürüntü yönündedir. Altın standart tanı yöntemi olarak kabul edilmesine karşın bazı dezavantajları vardır. En önemlisi sonuçlanmasının uzun sürmesi ve yüksek maliyettir. Maliyeti azaltmak için birkaç örneğin tek bir örnek gibi değerlendirilmesi yani havuzlandırılması tercih edilebilir. Bu dezavantajlara alternatif olarak hızlı antijen testleri göz önünde bulundurulabilir. Hızlı antijen testlerinin pek çok farklı piyasa formu vardır. Bu testlerin özgüllükleri ve duyarlılıklarına yönelik pek çok çalışma yapılmakta olup farklı değerler bulunmaktadır. Genel olarak özgüllüklerinin yüksek ve duyarlılığının ise daha düşük olduğu tespit edilmiştir. Hastalığın semptomatik tanısının zorluğu, bazı kişilerin hastalığı bulaştırmasına karşın semptom göstermemesi sorunları için toplum taramaları çözüm yolu olabilir. Yeni varyantların ortaya çıkması ile dünya genelinde test politikalarında değişiklikler ve farklı yaklaşımlar gözlenmiştir. Daha geniş kapsamlı tarama yapmak amacıyla testlerin tüm halka açık hale getirilmesi veya toplum taramaları yönünde çalışmalar gerçekleştirilmiştir. Ancak artan PCR test yükü dolayısıyla bazı ülkelerde test yapılacak kişilerin kısıtlanması veya hızlı antijen testleri gibi alternatif test yöntemlerinin ek olarak kullanılması gibi test politikalarının uygulanması gerekmiştir.

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Greater public health impact of COVID-19 antigen detection tests

Cited by 4 publications

References 8 publications

Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen

Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen

Rapid detection of SARS-CoV-2: The gradual boom of lateral flow immunoassay

Usage of Diagnosis and Screening Tests in New Coronavirus Disease (Covıd-19)

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