Green analysis of newly approved binary omeprazole/aspirin mixture in presence of aspirin impurity using ultra‐high‐performance liquid chromatography and thin‐layer chromatography methods

Abstract

Two green, simple, and accurate chromatographic methods were developed and validated for the simultaneous determination of omeprazole and aspirin mixture in the presence of salicylic acid, a major impurity of aspirin. Method A is a reversed‐phase ultra‐high‐performance liquid chromatography; the separation was performed on a C18 column, with a mobile phase composed of ethanol:0.1% aqueous solution of triethylamine acidified with orthophosphoric acid (pH 3) (30:70, v/v) at 0.15 mL/min flow rate and 230 nm. Omeprazole, aspirin, and aspirin impurity retention times were 7.47, 4.40, and 5.13 min, respectively. Good linearity was achieved in the concentration ranges of 5–80, 5–85, and 3–50 μg/mL for the three mentioned components, respectively. Method B is thin‐layer chromatography (TLC) where silica gel TLC F254 plates were utilized to achieve separation using ethanol:ethyl acetate (2:8, v/v) as a developing system at 240 nm. The resulted Rf values were 0.83, 0.65, and 0.23 for omeprazole, aspirin, and impurity, respectively. The concentration ranges of 0.1–3 μg/band for the three drugs showed good linearity. The proposed methods are eco‐friendly and greener when compared to the already reported method (Microchemical Journal, 152, 104350). This is the first use of TLC method for the determination of the three drugs. International Council for Harmonization (ICH) guidelines were followed to ensure the validity of developed methods.

Spider diagram and sustainability evaluation of UV-methods strategy for quantification of aspirin and sildenafil citrate in the presence of salicylic acid in their bulk and formulation

“…1 A, which is an antiplatelet drug that lowers the risk of cardiovascular diseases mortality [ [6] , [7] , [8] ]. Some methods used for the quantification of ASA like HPLC [ 9 ], spectrophotometric [ 10 ], UHPLC [ 11 ], TLC [ 11 ], and UPLC [ 12 ]. These methods are shown in Table 1 .…”

“… Drug analyzed Method studied Publication year Reference Aspirin + Methocarbamol in the apparition of guaifenesin and salicylic acid. HPLC-DAD method 2019 [ 9 ] Aspirin + Omeprazole in the apparition of Salicylic Acid Spectrophotometric methods 2022 [ 10 ] Aspirin + Omeprazole in the presence of salicylic acid UHPLC and TLC 2021 [ 11 ] Aspirin + Salicylic acid + Salvianolic acid B UPLC-MS/MS 2021 [ 12 ] Sildenafil citrate + Dapoxetine + Vardenafil + Tadalafil Spectrofluorimetric and Spectrophotometric methods 2020 [ 15 ] Dapoxetine + Sildenafil Spectrophotometric methods 2022 [ 16 ] Sildenafil citrate Infrared Spectroscopic method 2021 [ 17 ] Sildenafil HPLC 2020 [ 18 ] Aspirin + Sildenafil citrate Spectrophotometric method 2015 [ 19 ] Aspirin + Sildenafil citrate Spectrophotometric methods 2022 [ 20 ] Aspirin + Sildenafil citrate + Salicylic acid HPLC 2014 [ ...…”

Improved performance of printed electrochemical sensors via cold atmospheric plasma surface modification

“…We have estimated the limit of detection (LOD) of the sensors toward nitrogen detection using the slopes of the calibration curves and residual standard deviation of the y -intercept from the regression of the calibration curve (SD). 49 By applying the equation of LOD = 3.3 SD/Slope, the LOD values were calculated to be 4 × 10 −5 and 1 × 10 −5 M for UT-ISE and PT-ISE, respectively.…”

Research progress on the impurities identification and determination in pharmaceuticals

“…Precision would be comparable to that of estimation via HPTLC, and the analytical method reduces the measurement cost, time and consumption of organic reagents. Eglal et al 60 is the first use of TLC method for the determination of aspirin, omeprazole and the impurity of aspirin (salicylic acid). TLC method is eco-friendly and greener when compared to the already reported method.…”