Controlled-atmosphere (CA) effects on chlorophyll and carotenoid concentrations in green beans
were studied. Green bean pods (cv. Perona) were stored at 8 °C and 98% relative humidity, with
and without controlled atmospheres (samples: air, 5% O2−3% CO2; 3% O2−3% CO2; 1% O2−3%
CO2), and then transferred to 20 °C to simulate ambient temperature. Green beans stored in air
at 8 °C had good appearance for 18 days, whereas an atmosphere of 1% O2−3% CO2 extended the
storage period to 22 days. Samples stored in other assayed atmospheres (5% O2−3% CO2 or 3%
O2−3% CO2) had the same shelf life as the air-stored sample (18 days). Maximum chlorophyll
accumulation in air-stored green beans took place at 6 days of storage, whereas the 1% O2−3% CO2
stored sample showed the maximum chlorophyll concentration at 13 days of storage, and no
significant increase of this pigment was observed in the first 6 days. Degradation of chlorophylls
a and b did not yield the corresponding pheophytin compounds, because the pheophytin content
also showed a significant decrease during storage. Green bean carotenoids showed similar changes
in chlorophyll concentrations during storage in air, 3% O2−3% CO2, and 5% O2−3% CO2.
Keywords: Green beans; cold storage; controlled atmosphere; pigments; decay; shelf life