Green biomanufacturing in recombinant collagen biosynthesis: trends and selection in various expression systems

Abstract

The rapid development of synthetic biology allows us to perform a heterologous expression of recombinant collagens in diverse expression systems (prokaryotic organisms, yeasts, plants, insects, mammalian and human cells, etc.).

Expression of Multicopy Tandem Recombinant Ginseng Hexapeptide in Bacillus subtilis and the Evaluation of Antiaging Activity

“…In the past decade, researchers have provided extensive scientific evidence supporting the health benefits of proteins and peptides obtained from food sources. In recent years, a growing number of small-molecule bioactive peptides have been demonstrated to improve lifespan and delay aging, including myostatin, palmitoyl tripeptide-5, collagen peptides, acetyl hexapeptide-3, and GOPs. In addition, these bioactive peptides can reduce oxidative stress and age-related diseases by eliminating excess radicals in the body .…”

Production and Functional Analysis of Collagen Hexapeptide Repeat Sequences in Pichia pastoris

“…3,4 It is mainly found in various tissues and organs of animals, and has attracted much attention due to its high nutritive value, good moisturization, degradability, biocompatibility, and low antigenicity, and has good application value in different industries such as pharmaceuticals, food, and daily chemicals. 5,6 So far, type I collagen is the most abundant structural protein in the human body, accounting for more than 90% of the organic volume of bone, and is also the major component of connective tissues such as skin, tendons, bone, and dentin. 7 Specific cellular functions of collagens are known to be generated by the interaction between specific epitopes displayed on the triple helix and collagen receptors (i.e., integrins, discoidal structural domain receptors, and platelet glycoprotein VI) 8 or other collagen-binding molecules.…”

“…The extracellular matrix (ECM) is a complex network composed of proteins, polysaccharides, and extracellular DNA. It plays a key role not only in providing structural support for tissues but also in various cellular processes. , Collagen is a vital structural protein and a major component of the ECM. , It is mainly found in various tissues and organs of animals, and has attracted much attention due to its high nutritive value, good moisturization, degradability, biocompatibility, and low antigenicity, and has good application value in different industries such as pharmaceuticals, food, and daily chemicals. , So far, type I collagen is the most abundant structural protein in the human body, accounting for more than 90% of the organic volume of bone, and is also the major component of connective tissues such as skin, tendons, bone, and dentin …”

Biosynthesis of a Functional Fragment of Human Collagen II in Pichia pastoris

“…4 Although the biosynthesis of recombinant COL has been commercialized, further research and development are required to enhance biosafety, yield, and functionality. 5 Thus, there is a need to identify new functional COL fragments as potential biomaterials.…”

“…Consequently, various expression systems, including insects, animal cells, plants, bacteria, and yeast, have been developed to produce recombinant COL. With the increasing usage of COL, the market is projected to reach 6.63 billion US dollars by 2025 . Although the biosynthesis of recombinant COL has been commercialized, further research and development are required to enhance biosafety, yield, and functionality . Thus, there is a need to identify new functional COL fragments as potential biomaterials.…”