Green Biosynthesis of Silver Nanoparticles Using Vallarai Chooranam and Their Potential Biomedical Applications

Sathiyaraj, Sivaji; Suriyakala, Gunasekaran; Gandhi, Arumugam Dhanesh; Saranya, S.; Santhoshkumar, Murali; Kavitha, P.; Babujanarthanam, Ranganathan

doi:10.1007/s10904-020-01683-7

Cited by 28 publications

(21 citation statements)

References 42 publications

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“…In our study this was evident from UV-Vis spectrum that the SPR was visible between 400 to 450 nm and the highest excitation of AgNPs was at 420 nm ( Fig. 1) due to vibrational movement of electrons at that wavelength depending on size and shape of SpNPs which is in line with other related studies 2,5,14,36 . Further, we observed that a higher concentration of the plant material (solution Y) to silver nitrate (solution X) exhibit a direct effect on the size and dispersion of the SpNPs.…”

Section: Discussionsupporting

confidence: 92%

“…Our synthesized SpNPs may have broad applications in biomedical field, for example they can be used for wound healing 61 , as anticoagulant, anticancer, and for anti-inflammatory biosensing applications 36 . Moreover, SpNPs may also have environmental applications 15 to reduce contaminants such as microbial pathogens and toxic compounds including dyes and heavy metals.…”

Section: Discussionmentioning

confidence: 99%

“…They also exhibit effective antimicrobial efficacy against fungal pathogens 31 . Recently, applications of AgNPs in other biomedical fields have also been reported, for instance, anticoagulant activity 32 , thrombolytic effect 33 , anti-filarial 34 , anti-leishmania 35 , antilarval, anthelmintic, antioxidant activity and anticancer 36 . The applications of AgNPs in biomedical and pharmaceuticals will continue to rise, owing to their potential industrial applications related to global health and eco-benign production requiring cheaper raw materials and cost-effective procedures.…”

Section: Introductionmentioning

confidence: 99%

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Salvadora persica mediated synthesis of silver nanoparticles and their antimicrobial efficacy

Arshad

Sami

Sadaf

et al. 2021

Sci Rep

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Silver nanoparticles (AgNPs) exhibit strong antimicrobial properties against many pathogens. Traditionally employed chemical methods for AgNPs synthesis are toxic for the environment. Here, we report a quicker, simpler, and environmentally benign process to synthesize AgNPs by using an aqueous ‘root extract’ of Salvadora persica (Sp) plant as a reducing agent. The synthesized Salvadora persica nano particles (SpNPs) showed significantly higher antimicrobial efficacy compared to earlier reported studies. We characterized SpNPs using UV–Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy (FE-SEM), Dynamic Light Scattering (DLS) and X-ray powder diffraction (P-XRD). UV–Vis spectrum showed the highest absorbance at 420 nm. FTIR analysis depicts presence of bond stretching including OH– (3300 cm−1), C=N– (2100 cm−1) and NH– (1630 cm−1) which are attributed in the involvement of phenolics, proteins or nitrogenous compounds in reduction and stabilization of AgNPs. TEM, FE-SEM and DLS analysis revealed the spherical and rod nature of SpNPs and an average size of particles as 37.5 nm. XRD analysis showed the presence of the cubic structure of Ag which confirmed the synthesis of silver nanoparticles. To demonstrate antimicrobial efficacy, we evaluated SpNPs antimicrobial activity against two bacterial pathogens (Escherichia coli (ATCC 11229) and Staphylococcus epidermidis (ATCC 12228)). SpNPs showed a significantly high inhibition for both pathogens and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were found to be 0.39 µg/mL and 0.78 µg/mL for E. coli while 0.19 µg/mL and 0.39 µg/mL for S. epidermidis respectively. Further, Syto 16 staining of bacterial cells provided a supplemental confirmation of the antimicrobial efficacy as the bacterial cells treated with SpNPs stop to fluoresce compared to the untreated bacterial cells. Our highly potent SpNPs will likely have a great potential for many antimicrobial applications including wound healing, water purification, air filtering and other biomedical applications.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Salvadora persica mediated synthesis of silver nanoparticles and their antimicrobial efficacy

Arshad

Sami

Sadaf

et al. 2021

Sci Rep

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“…Microbial and phyto-metabolites derived NPs are produced by an environmental friendly approach and have several biomedical applications including antimicrobial and cytotoxic effects (Garibo et al 2020). The facile synthesis of nanoparticles with desirable properties has led to increase in the applications of biosynthesized metal nanoparticles including in vivo sensing, bio-imaging, targeted therapy, diagnosis, enzyme immobilization, antibacterial and anti-cancerous applications (Ding et al 2015;Sathiyaraj et al 2020). Green synthesis or the plant extract driven fabrication and stabilization of metal nanoparticles is renewable, eco-friendly, economically viable, non-toxic and leads to biologically compatible nano-products with relatively high reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

2020

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The present study describes green synthesis of silver nanoparticles (AgNPs) and inulin hydrolyzing enzyme nanocomplexes (ENC) using Azadirachta indica (Ai) and Punica granatum (Pg) leaf extracts. Surface topology and physico-chemical characteristics of AgNPs were studied using surface plasmon resonance (SPR), FTIR, SEM, AFM and EDX analyses. Particle size analysis using dynamic light scattering and AFM studies revealed that Ai-AgNPs (76.4 nm) were spherical in shape having central bigger nano-regime with smaller surroundings while Pg-AgNPs (72.1 nm) and ENCs (Inulinase-Pg-AgNPs ~ 145 nm) were spherical particles having smooth surfaces. Pg-AgNPs exhibited significant photocatalysis of a thiazine dye, methylene blue. Both Ai-and Pg-AgNPs showed selective antibacterial action by inhibiting pathogenic Bacillus cereus, while the probiotic Lactobacillus strains remained unaffected. Ai-AgNPs showed potential anti-biofilm effect (30% viability) on B. cereus biofilms. Pg-AgNPs showed anti-cancer effect against human colon cancer cell lines (Caco-2) resulting in 40% cell death in 48 h. Enzymes (inulinase, L-asparaginase and glucose oxidase) were successfully immobilized onto nanoparticles together with the biogenic synthesis of AgNPs and recyclability of the Inulinase-Pg-AgNPs complex was demonstrated. The study elaborates characteristics of green synthesized nanoparticles and their potential applications as anti-cancer, antibacterial and antioxidant nano drugs that could be used in food and nutraceutical industries. Enzyme immobilization on AgNPs without any toxic cross-linker opens up newer possibilites in enzyme-nanocomplex research.

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“…The TiO 2 NPs have smaller size is the most activity sample against all the bacteria as compared to those calcined at higher temperature. This is due to the increase in the particles size, surface area decreased resulting a clear reduction in the reactive surfaces sites, which decrease the capability of nanoparticles to inhibit the growth of bacteria (Mahfooz‐Ur‐Rehman et al, 2019; Sathiyaraj, Suriyakala, Dhanesh, & Sivakumar, 2020). The TiO 2 sparingly dissolved in aqueous solution and release Ti 2+ ions, which alter the surface properties like free energy.…”

Section: Resultsmentioning

confidence: 99%

Structural and biological investigation of biogenically synthesized titanium dioxide nanoparticles: Calcination and characterization

Hafeez

Shamim

Ehsan

et al. 2021

Microscopy Res & Technique

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The antimicrobial drug resistance is increasing with the passage of time due to wide and improper use of broad spectrum drugs and the demand of the new drug increases day by day. The present study was planned to encounter this problem by synthesizing titanium dioxide nanoparticles (TiO2 NPs) by an eco‐friendly route using Cannabis sativa leaves extract. The synthesized TiO2 NPs were calcined at 100, 300, 600, and 900°C in a muffle furnace. The crystallographic parameters were studied by X‐ray diffraction and the phase transition occurred above 600°C. The surface morphology of the synthesized samples was studied by transmission electron microscopy (TEM), and scanning electron microscopy (SEM) and the particle size was measured through the ImageJ software. The elemental composition and purity of all the samples were studied by performing energy dispersive X‐ray (EDX). All the synthesized TiO2 NPs were tested for their antimicrobial effect against Gram‐positive and Gram‐negative bacteria using the agar well diffusion method. The activity was found higher against Gram‐negative bacteria and compared to Gram‐positive bacteria.

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Green Biosynthesis of Silver Nanoparticles Using Vallarai Chooranam and Their Potential Biomedical Applications

Cited by 28 publications

References 42 publications

Salvadora persica mediated synthesis of silver nanoparticles and their antimicrobial efficacy

Salvadora persica mediated synthesis of silver nanoparticles and their antimicrobial efficacy

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

Structural and biological investigation of biogenically synthesized titanium dioxide nanoparticles: Calcination and characterization

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