Green biosynthesis of titanium dioxide quantum dots using watermelon peel waste: antimicrobial, antioxidant, and anticancer activities

Ali, Omar M.; Hasanin, Mohamed S.; Suleiman, Waleed B.; Helal, Eman El-Husseiny; Hashem, Amr H.

doi:10.1007/s13399-022-02772-y

Cited by 61 publications

(29 citation statements)

References 80 publications

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“…7) that showed one major broad band at 207 nm with absorbance 2.54 au, another minor band at 433 with absorbance 0.44 au which is a typical band for Ag-NPs as mentioned in previous studies [61]. Moreover, the broadness of the band may be related to the method [45].…”

Section: Taxonomic Characterizations Of the Actinomycete Isolate Azha...mentioning

confidence: 60%

“…For such purpose, an aliquot of aqueous suspension of Ag-NPs was transferred onto a carbon coated copper grid and allowed to dry at room temperature, the grid was then scanned using TEM (JEOL, JEM2100, Electron Microscope, TEMHR, Japan), TEM operated at a voltage of 80 kV. The size of biosynthesized Ag-NPs was analyzed using Image Analysis docu software program [45].…”

Section: Molecular Identi Cation Of Most Antagonistic Actinomycetes I...mentioning

confidence: 99%

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Ecofriendly biosynthesis of Ag-NPs by Streptomyces griseus eradicating Candida albicans associated with different types of dysspermatism

El-Enain

Abed

Helal

et al. 2022

Preprint

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Silver nanoparticles Ag-NPs have potent biological properties such as antibacterial, antifungal, anti-viral and anti-inflammatory activities. A simple, eco-friendly, inexpensive method was conducted in order to synthesize silver nanoparticles by actinomycetes. A total of 17 actinomycetes isolates were recovered from serially diluted compost sample and subsequently examined for their capabilities to produce Ag-NPs. Alongside, Candida albicans was isolated from human semen that affect male infertility, and subsequently submitted to susceptibility test using the well diffusion method. It was found that the Ag-NPs biosynthesized by Streptomyces griseus Azhar.S2 have high zone of inhibition against Candida albicans which was recorded by measuring the diameter of inhibition zone that was 20.5 ± .05 mm. The biosynthesized Ag-NPs had been characterized using UV-vis spectroscopy, FTIR, EDX and TEM. Conclusively, Candida albicans isolated from different types of dysspermatism human semen was highly eradicated by microbially synthesized Ag-NPs from Streptomyces griseus Azhar.S2.

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Section: Taxonomic Characterizations Of the Actinomycete Isolate Azha...mentioning

confidence: 60%

Section: Molecular Identi Cation Of Most Antagonistic Actinomycetes I...mentioning

confidence: 99%

Ecofriendly biosynthesis of Ag-NPs by Streptomyces griseus eradicating Candida albicans associated with different types of dysspermatism

El-Enain

Abed

Helal

et al. 2022

Preprint

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“…Six vehicle controls with media orr0.5% DMSO were ran for each 96ewell plate as aacontrol. After incubationffor 24 h, numbers of viable cells were determined by the MTT test [20][21][22]. The viability and inhibition percentages were calculated according to these equations:…”

Section: In-vitro Anticancer Activity Assaymentioning

confidence: 99%

Purification, Characterization and anticancer activity of L-methionine γ-lyase from thermo-tolerant Aspergillus fumigatus

et al. 2023

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Purification of L-methionine γ-lyase (MGL) from A. fumigatus was sequentially conducted using heat treatment and gel filtration, resulting in 3.04 of purification fold and 73.9% of enzymatic recovery. The molecular mass of the purified MGL was approximately apparent at 46 KDa based on SDS-PAGE analysis. The enzymatic biochemical properties showed a maximum activity at pH 7 and exhibited plausible stability within pH range 5.0–7.5; meanwhile the highest catalytic activity of MGL was observed at 30–40 °C and the enzymatic stability was noted up to 40 °C. The enzyme molecule was significantly inhibited in the presence of Cu2+, Cd2+, Li2+, Mn2+, Hg2+, sodium azide, iodoacetate, and mercaptoethanol. Moreover, MGL displayed a maximum activity toward the following substrates, L-methionine < DL-methionine < Ethionine < Cysteine. Kinetic studies of MGL for L-methioninase showed catalytic activity at 20.608 mM and 12.34568 µM.min−1. Furthermore, MGL exhibited anticancer activity against cancerous cell lines, where IC50 were 243 ± 4.87 µg/ml (0.486 U/ml), and 726 ± 29.31 µg/ml (1.452 U/ml) against Hep-G2, and HCT116 respectively. In conclusion, A. fumigatus MGL had good catalytic properties along with significantly anticancer activity at low concentration which makes it a probably candidate to apply in the enzymotherapy field.

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“…SEM technique was performed stepwise including fixation by osmium tetraoxide (OsO 4 ), followed by tissue processor (Sciences tissue processor model Lynx) for dehydration through immersion in gradual concentrations of ethyl alcohol followed by gradual concentrations of acetone. The third step involved the critical point dryer (EMS 850 apparatus) in which acetone was replaced by carbon dioxide, mounted on stubs to be ready for the gold coating in order to good conductivity by diode gold sputter coater (SPI Module™ Sputter Coater), and finally, the fugal specimen getting ready to be examined by highvacuum mode of a JEOL JSM-5500LV Scanning Electron Microscope [27,28].…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

A multi-aspect analysis of two analogous aspergillus spp. belonging to section Flavi: aspergillus flavus and aspergillus oryzae

Suleiman

2023

BMC Microbiol

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Microfungal isolates were routinely identified depending on both macro and micro morphological characteristics, sometimes, some fungal isolates appeared to be similar and such cases caused severe confusion for mycologists during the preliminary identification. During our previous studies dealing with isolation of fungi for some biotechnological applications; two mystifying species Aspergillus flavus and Aspergillus oryzae showed similar cultural and macroscopic features. Therefore, the current study aimed to easily distinguish between these two species depending on simple approaches which are routinely followed by a large segment of researchers. Investigation of the macroscopic features was performed to check the fungal growth on four different media (PDA, MEA, YES, and CYA) followed by microscopic examination using an ordinary light microscope, and scanning electron microscope SEM. Also, screening of secondary metabolites for both strains was preliminarily identified to find out the difference between their metabolic profiles. Finally, ITS rDNA was involved to clarify the molecular differences along their partial sequence. Conclusively, the BLAST strategy confirmed the similarity of ITS rDNA segments of both fungal strains that supported our hypothesis. The color of the fungal growth is a very critical factor whereas it is extensively influenced by the type of cultivation media. Accordingly, the YES medium was an inspiring tool assisting in prompt differentiation during the culture investigation step whereas A. oryzae and A. flavus appeared significant mustard yellow and olive green respectively. During the microscopic examination, the CYA medium also had a robust effect on the formation of the conidial chain whereas the knit long chain was observed in A. oryzae while the conidia appeared scattered and not in a chain in the case of A. flavus. Likewise, both two strains possessed different metabolic profiles where A. oryzae is not an Afla toxin producer, unlike A. flavus.

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Green biosynthesis of titanium dioxide quantum dots using watermelon peel waste: antimicrobial, antioxidant, and anticancer activities

Cited by 61 publications

References 80 publications

Ecofriendly biosynthesis of Ag-NPs by Streptomyces griseus eradicating Candida albicans associated with different types of dysspermatism

Ecofriendly biosynthesis of Ag-NPs by Streptomyces griseus eradicating Candida albicans associated with different types of dysspermatism

Purification, Characterization and anticancer activity of L-methionine γ-lyase from thermo-tolerant Aspergillus fumigatus

A multi-aspect analysis of two analogous aspergillus spp. belonging to section Flavi: aspergillus flavus and aspergillus oryzae

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