Green Designer Solvents for Boosting the Extraction of Biocompounds from Eugenia pyriformis Cambess Leaves

Abstract

This work aimed at evaluating eutectic mixtures as green and designer solvents to extract bioactive compounds from Eugenia pyriformis Cambess (EPC) leaves. First, it was defined through the Folin–Ciocalteu (F–C) reducing capacity and antioxidant activity the optimized conditions of solid–liquid extraction (SLE). Consecutively, this condition (65 °C, 1:30 (m/v) solid–liquid ratio, and 150 min of process) was applied to evaluate the effect of the eutectic mixture-forming compounds in the SLE. The results showed that the lactic acid-based eutectic mixture was the most suitable solvent to preserve bioactive compounds from EPC leaves. Moreover, according to F–C analysis, the lactic acid-based eutectic mixtures boosted the SLE (163.14 ± 5.81 mg gallic acid/g sample). Regarding the phenolic profile, the EPC leaves showed the presence of quercetin, naringin, rutin, catechin, and gallic acid. These findings proved the eutectic mixtures and their forming compounds designer ability to extract valuable biocompounds from EPC leaves.

Optimization of green extraction of high‐value components from Eugenia uniflora leaves: Thermal stability and in‐vitro biological activity

“…[38,39] Besides, DES as a solvent to obtain bioactive compounds from natural matrices, similar to EUL, also resulted in high indexes of antioxidant activities. [36,40,41] The higher activity observed in [Ch]Cl: LA extracts corroborates the results obtained from the TPC.…”

“…The sample amount reduction increases the extraction of bioactive compounds due to a better exposition of the target compound in the solvent. [ 36 ] Thus, the first analysis considering the TPC of the extracts demonstrated that the ideal conditions to obtain bioactive compounds from EUL were: 65°C, 150 min, and a solid:liquid ratio of 1:30.…”

“…Table S3). Mafra and co‐workers [ 36,47 ] also described a significantly higher half‐life for DES‐based extracts of uvaia and soursop leaves than conventional solvents. The knowledge of this kinetic parameter is essential to determine the changes in the bioactivity of the extracts under several process conditions.…”