Green Extracellular Synthesis of the Silver Nanoparticles Using Thermophilic Bacillus Sp. AZ1 and its Antimicrobial Activity Against Several Human Pathogenetic Bacteria

Deljou, Ali; Goudarzi, Samad

doi:10.15171/ijb.1259

Cited by 86 publications

(36 citation statements)

References 41 publications

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“…Silver (Ag) signal comes from the AgNPs and the atomic percentage of silver was 79.96%. In this connection, the absorbance of the metallic AgNPs generally shows an absorption peak approximately at 3 KeV (Deljou and Goudarzi, 2016). In addition to Ag, there were also some other peaks.…”

Section: Edx Spectroscopy Analysismentioning

confidence: 88%

BIOSYNTHESIS, OPTIMIZATION AND CHARACTERIZATION OF SILVER NANOPARTICLES BIOSYNTHESIZED BY Bacillus subtilis ssp spizizenii MT5 ISOLATED FROM HEAVY METALS POLLUTED SOIL

El‐Saadony

El-Wafai

El-Fattah

et al. 2018

Zagazig Journal of Agricultural Research

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Nanotechnology and nanoparticles (NPs) researches have attracted a lot of interest in recent decades, and there is growing attention to find more effective ways for their synthesis. The use of biological approach, (using various microorganisms), as bio-nanofactories provides a clean and promising alternative process for the fabrication of silver nanoparticles. This study confirmed the production of silver nanoparticles (AgNPs) by a cost effective, safe and environment-friendly technique using silver nitrate and supernatants of the bacterium Bacillus subtilis ssp spizizenii MT5 as a bio reducing agent. Supernatants of the tested microbe growing on nutrient broth (NB) were used for fabrication of AgNPs. Some parameters of optimization i.e., incubation time, silver nitrate concentration, mixing ratio of culture supernatant and silver nitrate, media type, temperature degree and pH level were studied. The biosynthesis of AgNPs in the cell extract filtrate was confirmed and characterized by biophysical methods using the advanced available instruments. The determined conditions for the bioinspired synthesis of AgNPs revealed that incubation time was 40 h, silver nitrate concentration was 3mM, supernatant and silver nitrate ratio was 1:4, medium type was nutrient broth (NB), agitation speed was 160 rpm, temperature degree was 35°C and pH level was 7. Characterizations of the produced bio silver nanoparticles were done using the advanced available methods. The ultraviolet-visible spectrum showed an absorption peak at 420 nm. Transmission electron microscopy (TEM) showed that the mean diameter of the formed AgNPs was 38 to 49 nm. Powder X-ray diffraction (XRD) showed that the particles are crystalline in nature, with a facecentered spherical structure. Dynamic light scattering (DLS) and Zeta potential analysis showed that the average AgNPs size was 31.42 nm and the zeta potential was-20.8mV, Fourier Transform Infrared Spectroscopy analysis (FT-IR) confirmed the presence of elemental silver and the dual function of biomolecule responsible for the bio reduction and stabilization of AgNPs in the reaction mixtures. The scanning electron microscopy (SEM) micrograph indicated that produced AgNPs are spherical in shape. However, it also showed an indeterminate morphology. Energy-dispersive X-ray spectroscopy (EDX) exhibited strong signal in the silver region which confirms the formation of AgNPs.

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Section: Edx Spectroscopy Analysismentioning

confidence: 88%

BIOSYNTHESIS, OPTIMIZATION AND CHARACTERIZATION OF SILVER NANOPARTICLES BIOSYNTHESIZED BY Bacillus subtilis ssp spizizenii MT5 ISOLATED FROM HEAVY METALS POLLUTED SOIL

El‐Saadony

El-Wafai

El-Fattah

et al. 2018

Zagazig Journal of Agricultural Research

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“…(9 mm). Deljou and Goudarzi [1] reported the silver nanoparticles showed the highest activity against the E. coli with 22 mm of zone of growth inhibition while other pathogens were also affected by AgNPs but shown the lower range of inhibition. Cyanobacterium Oscillatoria sp.…”

Section: Antimicrobial Activity Of Synthesized Silver Nanoparticlesmentioning

confidence: 99%

“…One of the important features of nanotechnology is the synthesis of nanoparticles of different sizes, shape using new chemical, physical as well as biological methods. The chemical methods for the synthesis of nanoparticles may produce a large number of hazardous byproducts which is harmful to environment [1]. In last one-decade various metal oxides are used for synthesis of biological nanoparticles with number of biological applications [2].…”

Section: Introductionmentioning

confidence: 99%

Extracellular Biosynthesis of Silver Nanoparticles using Bacterial Isolate from Saline Soil

Pawar¹,

.²,

Gaikwad³

et al. 2020

JNST

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Nanotechnology is now known to be an area related to research and technology development. Nanoparticles have potential applications in the field of electronics, medicine and health. Nanotechnology is also proved in the medical field including therapeutics, drug discovery and diagnosis of diseases. An attempt has been made to isolate and identify the bacteria from saline soil and screening of all these bacteria for the synthesis of silver nanoparticles. Different bacterial cultures were isolated from saline soil samples from Baramati region. Isolated cultures were screened for extracellular biosynthesis of silver nanoparticles. The color change (white to brown) was observed after 72 hours of incubation. Among those bacteria the bacterial culture which showed absorption peaks in the range of 420-430 nm was selected for further characterization. Selected culture was identified using phenotypic and genotypic characteristics and was found to be Bacillus cereus. Characterization of nanoparticles was done using UV-visible spectral analysis, FTIR, SEM and XRD. The preparation showed characteristic absorption peak at 433 nm. SEM analysis illustrated that the silver nanoparticles formed were fairly uniform in size with a hexagonal shape and an average diameter of 94 nm. FTIR spectra revealed the presence of amino-methyl stretching groups and carbonyl groups. The antibacterial activity of the synthesized AgNPs was observed against human pathogens. The studies have indicated the potential of bacterial synthesized silver nanoparticles as bactericidal against human pathogens.

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“…The bacterial strain E. coli isolated from urine sample synthesis silver nanoparticles and exhibits significant antibacterial activity against Bacillus subtilis and Klebsiella pneumonia 92 . The bacterial strains isolated from oil-contaminated soil sites also have the potential to produce silver nanoparticles synthesis at 85 °C and pH value 7.0 93 95 . There are some actinobacteria like Streptomyces fulvissimus are also used for the green synthesis of nanoparticles 96 .…”

Section: Nanoparticles Synthesis By Plantsmentioning

confidence: 99%

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IJPSR

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Green Extracellular Synthesis of the Silver Nanoparticles Using Thermophilic Bacillus Sp. AZ1 and its Antimicrobial Activity Against Several Human Pathogenetic Bacteria

Cited by 86 publications

References 41 publications

BIOSYNTHESIS, OPTIMIZATION AND CHARACTERIZATION OF SILVER NANOPARTICLES BIOSYNTHESIZED BY Bacillus subtilis ssp spizizenii MT5 ISOLATED FROM HEAVY METALS POLLUTED SOIL

BIOSYNTHESIS, OPTIMIZATION AND CHARACTERIZATION OF SILVER NANOPARTICLES BIOSYNTHESIZED BY Bacillus subtilis ssp spizizenii MT5 ISOLATED FROM HEAVY METALS POLLUTED SOIL

Extracellular Biosynthesis of Silver Nanoparticles using Bacterial Isolate from Saline Soil

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