Green fluorescent protein inspired fluorophores

Kong, Jia; Wang, Yuefei; Qi, Wei; Ming, Huang; Su, Rongxin; He, Zhimin

doi:10.1016/j.cis.2020.102286

Cited by 43 publications

(28 citation statements)

References 150 publications

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“…Thus, we can visualize it as a paint bucket with pigment. The outer wall of the bucket effectively prevents its influence on the spatial structure of the target protein ( 54 – 56 ). The GFP does not require any reaction substrate and cofactor, has no species restriction, and can be expressed in a variety of biological cells to emit stable fluorescence.…”

Section: Discussionmentioning

confidence: 99%

Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

Zhang

Chen

et al. 2021

Front. Vet. Sci.

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Bovine mastitis is perplexing the dairy industry since the initiation of intensive dairy farming, which has caused a reduction in the productivity of cows and an escalation in costs. The use of antibiotics causes a series of problems, especially the formation of bacterial antimicrobial resistance. However, there are limited antibiotic-free therapeutic strategies that can effectively relieve bacterial infection of bovine mammary glands. Hence, in this study, we constructed a mammary gland tissue-specific expression vector carrying the antimicrobial peptide of bovine-derived tracheal antimicrobial peptide (TAP) and evaluated it in both primary bovine mammary epithelial cells (pBMECs) and mice. The results showed that the vector driven by the β-lactoglobulin gene (BLG) promoter could efficiently direct the expression of TAP in pBMECs and the mammary gland tissue of mice. In addition, significant antibacterial effects were observed in both in vitro and in vivo experiments when introducing this vector to bovine-associated Staphylococcus aureus-treated pBMECs and mice, respectively. This study demonstrated that the mammary gland tissue-specific expression vector could be used to introduce antimicrobial peptide both in in vitro and in vivo and will provide a new therapeutic strategy in the treatment of bovine mastitis.

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Section: Discussionmentioning

confidence: 99%

Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

Zhang

Chen

et al. 2021

Front. Vet. Sci.

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“…[ 12 ] Thus, to construct the bioavailable fluorophore with dynamic properties, [ 13 ] peptides constructed by aromatic residues may be one of the best candidates owing to their intrinsic fluorescent property, biocompatibility, and capacity to assemble into a variety of functional nanostructures. [ 14 ]…”

Section: Introductionmentioning

confidence: 99%

Color‐Tunable Fluorescent Hierarchical Nanoassemblies with Concentration‐Encoded Emission

Kong

Zhao

et al. 2022

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Cephalopods possess a dynamic coloration behavior to change their iridescence due to the concentration‐induced optical properties of chromatophores and hierarchical assembly of reflectin. However, cephalopods rarely have iridescence in the darkfield. It would be interesting to develop color‐tunable fluorescent hierarchical nanoassemblies with concentration‐encoded emission. Herein, to construct the bioavailable fluorophore with dynamic coloration properties, a histidine‐rich peptide is designed, which can self‐assemble into hierarchical nanoassemblies stabilized by hydrogen bonds and π–π stacking interactions. The peptidyl nanoassemblies emit fluorescent iridescence, encompassing the blue to orange region due to the assembly‐induced emission. The fluorescence of histidine‐rich peptides is color‐tunable and reversible, which can be dynamically controlled in a concentration‐encoded mode. Due to the coloration ability of histidine‐rich peptides, fluorescent polychromatic human cells are developed, highlighting its potential role as a fluorescent candidate for future applications such as bioimaging, implantable light‐emitting diodes, and photochromic camouflage.

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“…Self-assembled peptides with remarkable photoactive properties, such as photocatalysis and fluorescence, can find promising applications in bioimaging, sensing, cell therapy, and optical devices. − Circularly polarized luminescence (CPL) is defined as the difference in left-handed and right-handed circular polarization intensity of fluorescence generated when the luminescent chiral molecules are excited by light, which has potential applications in sensors, − asymmetric catalysis, , and optical devices. , Generally, the materials with CPL activity could be fabricated by adding achiral luminescent molecules to the chiral matrix, such as quantum dots and aggregation-induced emission (AIE) molecules. − In recent years, the introduction of chiral amino acids or amino acid derivatives as a chiral molecule to induce CPL applications has been reported. − For example, Tang et al synthesized Silole-Leu with CPL by introducing chiral leucine pendants to the silole scaffold . Liu and co-workers revealed that nanohelix structures could transfer chirality to an achiral acceptor to form a composite nanohelix, which showed CPL. , The protein amyloid fibrils with intercalated thioflavin T (THT) also show induced CPL .…”

Section: Introductionmentioning

confidence: 99%

Self-Assembly of Peptide Hierarchical Helical Arrays with Sequence-Encoded Circularly Polarized Luminescence

Zhang

Wang

et al. 2021

Nano Lett.

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Self-assembled peptide materials with sequence-encoded properties have attracted great interest. Despite their intrinsic chirality, the generation of circularly polarized luminescence (CPL) based on the self-assembly of simple peptides has been rarely reported. Here, we report the self-assembly of peptides into hierarchical helical arrays (HHAs) with controlled supramolecular handedness. The HHAs can emit full-color CPL signals after the incorporation of various achiral fluorescent molecules, and the g lum value is 40 times higher than that of the CPL signal from the solutions. By simply changing the amino acid sequence of the peptides, CPL signals with opposite handedness can be generated within the HHAs. The peptide HHAs can provide hydrophobic pockets to accommodate the fluorescent molecules with helical arrangement through strong aromatic stacking interactions, which are responsible for the CPL signals. This work provides a pathway to construct highly ordered chiral materials, which have broad applications in the chiroptical field.

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Green fluorescent protein inspired fluorophores

Cited by 43 publications

References 150 publications

Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

Color‐Tunable Fluorescent Hierarchical Nanoassemblies with Concentration‐Encoded Emission

Self-Assembly of Peptide Hierarchical Helical Arrays with Sequence-Encoded Circularly Polarized Luminescence

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