Green-fruited Solanum habrochaites lacks fruit-specific carotenogenesis due to metabolic and structural blocks

Kilambi, Himabindu Vasuki; Manda, Kalyani; Rai, Avanish; Charakana, Chaitanya; Bagri, Jayram; Sharma, Rameshwar; Sreelakshmi, Yellamaraju

doi:10.1093/jxb/erx288

Cited by 25 publications

(22 citation statements)

References 61 publications

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“…Our observations suggest that primitive green-fruited species may have retained an ancestral expression pattern for each analyzed sucrose cleavage gene, as was also demonstrated for carotenoid metabolism genes (Kilambi et al, 2017).…”

Section: Resultssupporting

confidence: 75%

“…Fruits of red-fruited species accumulate carotenoids at high levels in carotenoid sequestering substructures inside chromoplasts (Li, Van Eck, 2007). Fruit carotenogenesis coincides with the chloroplast into chromoplast transformation (Kilambi et al, 2017). In green-fruited species, fruits do not have orange/red color due to the numerous blocks of fruit-specific chromoplast formation and carotenoid synthesis (Kilambi et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Fruit carotenogenesis coincides with the chloroplast into chromoplast transformation (Kilambi et al, 2017). In green-fruited species, fruits do not have orange/red color due to the numerous blocks of fruit-specific chromoplast formation and carotenoid synthesis (Kilambi et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Up-regulation of the vacuolar invertase TAI gene may contribute to the accumulation of carotenoids in tomato fruits

2019

Plant Genetic Resources for Breeding and Producing Functional Nutraceutical Food

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Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

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Up-regulation of the vacuolar invertase TAI gene may contribute to the accumulation of carotenoids in tomato fruits

2019

Plant Genetic Resources for Breeding and Producing Functional Nutraceutical Food

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“…Tomato species ( Solanum section Lycopersicon) are considered a good model to study fleshy fruit ripening, including coloration and evolution of the underlying genetic mechanisms, because of their significant phenotypic diversity [ 30 ]. All three PSY genes have been detected in cultivated tomato S. lycopersicum ; however, there are few studies on PSY enzymes in wild tomatoes, which mostly indicate that fruit color depends on PSY1 expression [ 31 , 32 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Regulation of Phytoene Synthase PSY1 During Fruit Carotenogenesis in Cultivated and Wild Tomato Species (Solanum section Lycopersicon)

Efremov

Слугина

Щенникова

et al. 2020

Plants

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In plants, carotenoids define fruit pigmentation and are involved in the processes of photo-oxidative stress defense and phytohormone production; a key enzyme responsible for carotene synthesis in fruit is phytoene synthase 1 (PSY1). Tomatoes (Solanum section Lycopersicon) comprise cultivated (Solanum lycopersicum) as well as wild species with different fruit color and are a good model to study carotenogenesis in fleshy fruit. In this study, we identified homologous PSY1 genes in five Solanum section Lycopersicon species, including domesticated red-fruited S. lycopersicum and wild yellow-fruited S. cheesmaniae and green-fruited S. chilense, S. habrochaites and S. pennellii. PSY1 homologs had a highly conserved structure, including key motifs in the active and catalytic sites, suggesting that PSY1 enzymatic function is similar in green-fruited wild tomato species and preserved in red-fruited S. lycopersicum. PSY1 mRNA expression directly correlated with carotenoid content in ripe fruit of the analyzed tomato species, indicating differential transcriptional regulation. Analysis of the PSY1 promoter and 5′-UTR sequence revealed over 30 regulatory elements involved in response to light, abiotic stresses, plant hormones, and parasites, suggesting that the regulation of PSY1 expression may affect the processes of fruit senescence, seed maturation and dormancy, and pathogen resistance. The revealed differences between green-fruited and red-fruited Solanum species in the structure of the PSY1 promoter/5′-UTR, such as the acquisition of ethylene-responsive element by S. lycopersicum, could reflect the effects of domestication on the transcriptional mechanisms regulating PSY1 expression, including induction of carotenogenesis during fruit ripening, which would contribute to red coloration in mature fruit.

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“…On the other hand, accessions south of the AHZ in Peru are often confined to isolated river valleys and experience more uniform environments with regard to altitude and temperature fluctuations. The diverse biotic and abiotic interactions and geographical features are associated with a range of metabolic and reproductive phenotypes in this species (Rick et al, 1979;Sacks & St. Clair, 1998;ten Have et al, 2007;Finkers et al, 2007;Gonzales-Vigil et al, 2012;Kim et al, 2012;Arms et al, 2015;Schilmiller et al, 2015;Markova et al, 2016;Broz et al, 2017b;Kilambi et al, 2017;Fan et al, 2017), some of which we assessed here.…”

Section: Discussionmentioning

confidence: 99%

Migration through a major Andean ecogeographic disruption as a driver of genotypic and phenotypic diversity in a wild tomato species

Landis

Miller

Broz

et al. 2020

Preprint

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The large number of species on our planet arises from the phenotypic variation and reproductive isolation occurring at the population level. In this study, we sought to understand the origins of such population-level variation in defensive acylsugar chemistry and mating systems in Solanum habrochaites – a wild tomato species found in diverse Andean habitats in Ecuador and Peru. Using Restriction-Associated-Digestion Sequencing (RAD-seq) of 50 S. habrochaites accessions, we identified eight population clusters generated via isolation and hybridization dynamics of 4-6 ancestral populations. Estimation of heterozygosity, fixation index, isolation by distance, and migration probabilities, allowed identification of multiple barriers to gene flow leading to the establishment of extant populations. One major barrier is the Amotape-Huancabamba Zone (AHZ) – a geographical feature in the Andes with high endemism, where the mountainous range breaks up into isolated microhabitats. The AHZ was associated with emergence of alleles for novel reproductive and acylsugar phenotypes. These alleles led to the evolution of self-compatibility in the northern populations, where alleles for novel defense-related enzyme variants were also found to be fixed. We identified geographical distance as a major force causing population differentiation in the central/southern part of the range, where S. habrochaites was also inferred to have originated. Findings presented here highlight the role of the diverse ecogeography of Peru and Ecuador in generating new, reproductively isolated populations, and enhance our understanding of the microevolutionary processes that lay a path to speciation.

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Green-fruited Solanum habrochaites lacks fruit-specific carotenogenesis due to metabolic and structural blocks

Abstract: The green-fruited Solanum habrochaites encodes a functional phytoene synthase 1 but fails to accumulate lycopene due to metabolic and structural blocks in chromoplast formation and carotenoid storage.

Cited by 25 publications

References 61 publications

Up-regulation of the vacuolar invertase TAI gene may contribute to the accumulation of carotenoids in tomato fruits

Up-regulation of the vacuolar invertase TAI gene may contribute to the accumulation of carotenoids in tomato fruits

Differential Regulation of Phytoene Synthase PSY1 During Fruit Carotenogenesis in Cultivated and Wild Tomato Species (Solanum section Lycopersicon)

Migration through a major Andean ecogeographic disruption as a driver of genotypic and phenotypic diversity in a wild tomato species

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