Green light effects on biological systems: a new biophysical phenomenon

Abstract

This paper reports a new phenomenon connected with the influence of green light (GL) on biological systems. Our experiments have revealed an antioxidant effect of GL on cells subjected to lethal doses of UV at the cellular level and a protective effect of GL on DNA denatured by UV, coupled with a structural modification of DNA macromolecules under GL irradiation, at the molecular level. Mouse melanocyte cultures are subjected to UV irradiations with L(50) fluxes of 16.0 J m(-2) s(-1). GL is obtained from a strontium aluminate pigment, which emits GL under UV activation. Cells grown in GL, prior to UV irradiation, present a clear surprising protective effect with surviving values close to the controls. A GL antioxidant effect is suggested to be mediated through GL influence on cellular water cluster dynamics. To test this hypothesis, reactive oxygen species (ROS) are determined in cell cultures. The results revealed a decrease of cellular ROS generation in the UV-irradiated samples protected by a previous 24 h of GL irradiation. At the DNA level, the same type of GL protection against UV damage is recorded by gel electrophoresis and by UV spectroscopy of the irradiated DNA molecules. Two physical methods, impedance spectroscopy and chronoamperometry, have revealed at the level of GL-irradiated DNA molecules spectral modifications that correlate with the UV spectroscopy results. The interaction between the chargeless photons and the field of water molecules from the cellular compartments is discussed in relation with the new field of macroscopic quantum coherence phenomena.

MYC amplifies gene expression through global changes in transcription factor dynamics

“…Interestingly, the Pi-mCherry line also displayed some increased growth when cultured under blue light. It has been previously reported that blue-green light may have antioxidant effects (Comorosan et al, 2009), have protective effects on protein subjected to UV irradiation (Espinoza and Mercado-Uribe, 2017), and initiate biophysical processes in cells such as increased wound healing and cell proliferation (Adamskaya et al, 2011;Rohringer et al, 2017).…”

Influence of green light illumination at night on biological characteristics of the oriental armyworm,Mythimna separata(Lepidoptera: Noctuidae)

“…Meanwhile, some researchers have suggested that when insects are exposed to UV irradiation, biological characteristics of these insects can be changed, because of damaging cells or DNAs of the body by reactive oxygen species (ROS) produced by UV irradiation (Zhang et al ., 2011; Ali et al ., 2016). However, in an animal body, green light illumination results in an antioxidant role on cells subjected to lethal doses of UV, in addition to, and has a protective role on DNAs denatured by UV light (Comorosan et al ., 2009). The green light illumination could result in weak oxidative stress in M. separata moths, and the moths under this light could restore the damage caused by this light stress (Kim et al ., 2018 b ).…”

Evaluation of the proper level of specific absorption rate of human blood for 532 nm laser in blood low-level laser therapy

“…Knowledge of its dielectric properties (i.e. dielectric constant, loss and conductivity) and the amount of energy absorbed by the blood, which is quantified using a measure called the specific absorption rate (SAR), is very important in the domain of nano-medicine and for rapid laser diagnosis and better therapeutic purposes [4,5,17]. Dielectric properties are the inherent molecular properties present in all materials capable of impeding electron movement and hence creating polarization within the substance.…”