Green method using water for lactose and lactulose extraction and determination in milk by high-performance liquid chromatography with refractive index detection

Gonzaga, Natalia; Watanabe, Lycio Shinji; Mareze, Juliana; Madeira, Tiago Bervelieri; Tamanini, Ronaldo; Rios, Edson Antônio; Nixdorf, Suzana Lucy; Beloti, Vanerli

doi:10.1016/j.lwt.2019.108288

Cited by 11 publications

(3 citation statements)

References 25 publications

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“…Carbohydrate quantification through the High Performance Liquid Chromatography -Refractive Index Detector (HPLC-RID) technique was performed with the methodology and equipment as described by Gonzaga et al (2019), which consisted of an LC-20AT bomb, with a DGU-20A gradient management system and degasser, and an SIL-20AC automatic injector. It was used Pb (II) -Aminex HPX-87P (300 mm × 7.8 mm, 9.0 µm, Bio-Rad, USA) cation exchange column, maintained at 80 °C in a CTO-20A oven, and detection was performed using the RID-10A refractive index detector, managed by the CBM -20A controller and commanded by the LC-Solution software (Version 1.21).…”

Section: High Performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

Artificial intelligence applied to enzymatic hydrolysis of lactose: improving the control of industrial processes

Santos

Carrilho

Abreu

et al. 2022

Semina: Ciênc. Agrár.

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Lactose is the main carbohydrate in milk, and its absorption occurs via enzymatic hydrolysis, generating glucose and galactose. Lactose intolerance is the reduction of intestinal hydrolysis capacity due to hypolactasia, which results in the need to consume dairy foods with low levels of this carbohydrate. β-galactosidase enzymes are used in dairy industries to hydrolyze lactose, thereby allowing intolerant consumers access to dairy products without the negative health implications. Alternative and official analytical methods are used to quantify the carbohydrate content resulting from enzymatic hydrolysis. The objective of this study was to evaluate the enzymatic hydrolysis of two distinct industrial enzymes produced by the microorganisms Bacillus licheniformis and Kluyveromyces lactis using three analytical methods: enzymatic method, cryoscopy, and high performance liquid chromatography (HPLC) using artificial intelligence to improve the control of the industrial processes. After adding the enzymes to skim milk, time kinetics was performed by collecting samples at time 0, every 10 min for 1 h, and every 30 min until the end of 5 h of hydrolysis. In 97% of the cases, a decrease in lactose concentration was observed by HPLC, followed by the deepening of the cryoscopic point. Glucose measurements by absorbance and HPLC quantification were correlated (r = 0.79; p < 0.01) but not concordant (p < 0.01). It was concluded that by means of artificial intelligence, it was possible to indirectly estimate lactose concentration using an algorithm that associates cryoscopy and glucose concentration.

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Section: High Performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

Artificial intelligence applied to enzymatic hydrolysis of lactose: improving the control of industrial processes

Santos

Carrilho

Abreu

et al. 2022

Semina: Ciênc. Agrár.

Self Cite

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“…Changes in the lactose content during milk fermentation are also essential in determining the quality of dairy products such as yoghurt, cheese, and cream 3 . The lactose content is also often used to indicate heat treatment intensity during the most common dairy heating processes because lactose is susceptible to isomerization 4 and Maillard 5 reactions when heated. Meanwhile, the lactose content also affects the dielectric loss factor of dairy products and thus the heating performance of the product 6 .…”

Section: Introductionmentioning

confidence: 99%

Hydration dominates dielectric spectroscopy‐based high accuracy quantification of lactose content in cow's milk

et al. 2023

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BACKGROUND Lactose is a critical factor in the quality of milk and dairy products. Achieving high accuracy and rapid detection of lactose content in cow's milk remains a challenge. Dielectric spectroscopy has emerged as a promising tool for detecting food components. We explored the effect of lactose content on the dielectric spectra of cow's milk and we propose a rapid analytical method for the quantitative determination of lactose content in cow's milk with high accuracy based on dielectric spectra. RESULTS We obtained the dielectric spectra of 316 cow's milk samples in the frequency range 20–4500 MHz and noticed a strong negative correlation between the lactose content and the value of the dielectric loss factor (ε″) below 1500 MHz. Lactose does not affect cow's milk dielectric properties by excluded volume effect, but dominates the effect on the dielectric properties of cow's milk by hydration. The support vector regression model based on the variable importance in projection has the best prediction performance for lactose content. Its root‐mean‐square error of prediction set and residual prediction deviation is 0.29 g kg−1 and 6.968, respectively, and its prediction performance is better than that of the currently reported near‐infrared (NIR) method and other methods. CONCLUSION Despite the weak polarity of lactose molecules, its hydration is a significant factor affecting the dielectric properties of milk. The present study provides a basis for high accuracy and rapid quantitative detection of lactose in cow's milk based on dielectric spectra. © 2023 Society of Chemical Industry.

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“…Lactose is used in the production of yogurts and cheeses, its solubility in aqueous medium is lower than that of other sugars, and its sweetness is 5-fold lower than that of sucrose. 1 In the body, the absorbed lactose is hydrolyzed under lactase (bgalactosidase) catalysis generating the respective monosaccharides, then transported to the bloodstream. The lack of this enzyme thus hinders absorption of lactose, causing gastrointestinal problems such as lactose intolerance, 2,3 and symptoms such as abdominal pain, colic, atulence and diarrhea.…”

Section: Introductionmentioning

confidence: 99%

A novel approach for lactose determination in cow's milk exploiting smartphone-based digital-image photometry

Malho Alves,

Martins,

Rocha

2023

Anal. Methods

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Green method using water for lactose and lactulose extraction and determination in milk by high-performance liquid chromatography with refractive index detection

Cited by 11 publications

References 25 publications

Artificial intelligence applied to enzymatic hydrolysis of lactose: improving the control of industrial processes

Artificial intelligence applied to enzymatic hydrolysis of lactose: improving the control of industrial processes

Hydration dominates dielectric spectroscopy‐based high accuracy quantification of lactose content in cow's milk

A novel approach for lactose determination in cow's milk exploiting smartphone-based digital-image photometry

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