Green micellar HPLC-fluorescence method for simultaneous determination of metoprolol and amlodipine in their combined dosage form: Application on metoprolol in spiked human plasma

Mabrouk, Mokhtar M.; Hammad, Sherin F.; El‐Malla, Samah F.; Elshenawy, Eman A.

doi:10.1016/j.microc.2019.03.084

Cited by 25 publications

(5 citation statements)

References 29 publications

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“…Ideal green analysis has a value of 100. The developed MLC method was found to have an analytical Eco‐Scale value of 83 indicating an excellent green HPLC method compared to the reported comparison HPLC method which has an analytical Eco‐Scale value of 75 [38, 55] as provided in Table S6. Also, AGREE scale [56] and GAPI tool [57, 58] were applied for method greenness evaluation as shown in Figures S10 and S11 respectively, both tools prove method greenness.…”

Section: Methodsmentioning

confidence: 99%

Green micellar stability‐indicating high‐performance liquid chromatography method for determination of rupatadine fumarate in the presence of its main impurity desloratadine: Oxidative degradation kinetics study

Amer

Habib

Hammad

et al. 2023

J of Separation Science

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A green micellar stability‐indicating high‐performance liquid chromatography method was developed for rupatadine fumarate determination in existence with its main impurity desloratadine. Separation was attained using Hypersil ODS column (150 × 4.6 mm, 5 μm), the micellar mobile phase consisted of 0.13 M sodium dodecyl sulfate, 0.1 M disodium hydrogen phosphate adjusted by phosphoric acid to pH 2.8 and 10% n‐butanol. The column was maintained at 45◦C and detection was carried out at 267 nm. A linear response was achieved over the range of 2–160 μg/ml for rupatadine and 0.4–8 μg/ml for desloratadine. The method was applied for rupatadine determination in alergoliber tablets and alergoliber syrup without the interference of methyl paraben and propyl paraben present as main excipients. Rupatadine fumarate revealed pronounced susceptibility to oxidation; further study of oxidative degradation kinetics was carried out. Rupatadine was found to follow pseudo‐first‐order kinetics when exposed to 10% H2O2 at 60 and 80°C and the activation energy was found to be 15.69 Kcal/mol. At a lower temperature (40°C), degradation kinetics regression was best fitted as a polynomial quadratic relationship, thus rupatadine oxidation at a lower temperature tends to adopt a second‐order kinetics rate. Oxidative degradation product structure was revealed using infrared and found to be rupatadine N‐oxide at all temperature values.

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Section: Methodsmentioning

confidence: 99%

Green micellar stability‐indicating high‐performance liquid chromatography method for determination of rupatadine fumarate in the presence of its main impurity desloratadine: Oxidative degradation kinetics study

Amer

Habib

Hammad

et al. 2023

J of Separation Science

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“…Also, as a result of the addition of the phosphate buffer, MT had its retention reduced. Buffer and SLS may have favored a micellar effect under MT, decreasing its retention [26,27]. After that, it was necessary to increase the concentration of the SLS to 6 mM in order to maintain the resolution between the analytes.…”

Section: Methods Developmentmentioning

confidence: 99%

A high‐performance liquid chromatography method for simultaneous quantification of metformin and ferulic acid in solid dosage forms

Júnior

Muniz

Almeida

et al. 2022

J of Separation Science

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Metformin is one of the most commonly used drugs in the world for the treatment of type 2 diabetes, while ferulic acid is a molecule that stands out for its antioxidant potential. Recent studies demonstrate hypoglycemic synergy between these molecules. The objective of this study is to develop and validate an analytical methodology by high‐performance liquid chromatography for the simultaneous quantification of these drugs in pharmaceutical formulations. The method used an octadecylsilane column and a mobile phase composed of 6 mM sodium lauryl sulfate in 15 mM phosphate buffer:ACN (65:35). Ferulic acid and metformin were monitored at 232 nm, with a mobile phase flow rate of 1 ml/min and oven temperature at 40°C. The method was linear in the range of 5–25 μg/ml for both molecules. In the presence of degradation products, satisfactory selectivity was achieved. Accuracy values were close to 100% and standard deviations in precision were less than 2%. In the robustness evaluation, the proposed variations did not interfere with the quantification. Therefore, it is concluded that the present method can be safely applied to the quality control of ferulic acid and metformin raw materials, as well as when they are combined in pharmaceutical formulations.

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“…However other anti-virals are estimated using different liquid chromatography techniques with green solvents such as gradient RP-HPLC-glibenclamide estimation using ethanol [7], Cefepime hydrochloride estimation using methanol [8], Rosuvastatin estimation using green solvent ethanol in RP-HPLC [9], Nevirapine and lamivudine using ethanol as solvent [10], Micellar chromatographic estimation of four antiviral drugs using Sodium Dodecyl Sulfate, Isopropanol, Triethylamine [11], Mixed micellar estimation of antiviral drugs using sodium lauryl sulfate [12] and other spectrophotometric-HPLC methods using green solvents [13] [14], famciclovir by RP-HPLC [15]. Certain green methods were reported using ethanol as a solvent for estimating nonantiviral drugs using HPLC such as deferasirox in human plasma [16], azosemide-micellar chromatography [17], impurities in deferasirox [18].…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating and Green Solvent-assisted Chromatographic Analysis of an Antiviral Drug

Archana,

Sumithra

2024

Preprint

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This study presents a green chromatographic method for estimating the Ganciclovir, demonstrating validation parameters in alignment with ICH guidelines and green chemistry principles. The green analytical technique utilized the ethanol and acidic water at pH 3.0 as solvents, with an optimized mobile phase ratio of (80:20) and a flow rate of 1.0ml/min with a zorbax eclipse plus C18 (4.6 x 150mm, 5mm). Ethanol is listed as a green solvent in U.S Tri EPA with (CAS 64-17-5), as well as economical with manageable waste compared to traditional solvents. Green Evaluation tools such as AGREE, GAPI, and COMPLEX GAPI were employed to assess the greenness scores of the method. The AGREE tool provided an evaluation of the greenness of the method while the GAPI and COMPLEX GAPI offered details about the assessment of the method’s adherence to the green chemistry principles. These tools were developed in adherence to 12 green analytical chemistry principles that provide a holistic approach toward qualitative and quantitative evaluations. The importance of greenness in analytical methods lies in reducing environmental impact, improving analyst safety, lowering cost and waste, and fostering environmental responsibility. The linearity range was 10-50μg/ml with a regression coefficient of less than or equal to 0.999 indicating a strong linearship. RSD values are consistently below 2% and recovery rates for ganciclovir ranged from 98% to 102% within acceptable ranges. Under forced degradation conditions the desired amount of degradation was exhibited 8.02% degradation in acid, 9.25% in alkaline hydrolysis, 4.02% degradation in peroxide, 5.42 % in thermal, and 6.38 % in photolytic studies which are within limits as per 5-20% as per ICH Q1B guidelines. Incorporating ICHQ1(R2) the method validation parameters are within the range and thoroughly addressed.

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Green micellar HPLC-fluorescence method for simultaneous determination of metoprolol and amlodipine in their combined dosage form: Application on metoprolol in spiked human plasma

Cited by 25 publications

References 29 publications

Green micellar stability‐indicating high‐performance liquid chromatography method for determination of rupatadine fumarate in the presence of its main impurity desloratadine: Oxidative degradation kinetics study

Green micellar stability‐indicating high‐performance liquid chromatography method for determination of rupatadine fumarate in the presence of its main impurity desloratadine: Oxidative degradation kinetics study

A high‐performance liquid chromatography method for simultaneous quantification of metformin and ferulic acid in solid dosage forms

Stability Indicating and Green Solvent-assisted Chromatographic Analysis of an Antiviral Drug

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