Green propolis extract attenuates acute kidney injury and lung injury in a rat model of sepsis

Abstract

Sepsis is the leading cause of acute kidney injury (AKI) and lung injury worldwide. Despite therapeutic advances, sepsis continues to be associated with high mortality. Because Brazilian green propolis (GP) has promising anti-inflammatory, antioxidant, and immunomodulatory properties, we hypothesized that it would protect kidneys and lungs in rats induced to sepsis by cecal ligation and puncture (CLP). Male Wistar rats were divided into groups—control (sham-operated); CLP (CLP only); and CLP + GP (CLP and treatment with GP at 6 h thereafter)—all receiving volume expansion and antibiotic therapy at 6 h after the procedures. By 24 h after the procedures, treatment with GP improved survival, attenuated sepsis-induced AKI, and restored renal tubular function. Whole-blood levels of reduced glutathione were higher in the CLP + GP group. Sepsis upregulated the Toll-like receptor 4/nuclear factor-kappa B axis in lung and renal tissues, as well as increasing inflammatory cytokine levels and macrophage infiltration; all of those effects were attenuated by GP. Treatment with GP decreased the numbers of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling-positive cells in renal and lung tissue, as well as protecting the morphology of the renal mitochondria. Our data open the prospect for clinical trials of the use of GP in sepsis.

Self-developed NF-κB inhibitor 270 protects against LPS-induced acute kidney injury and lung injury through improving inflammation

“…Most intensive care unit patients will die from multiple rather than individual organ failure (Vincent, 2011). Sepsis is the leading cause of both AKI and ALI, and sepsis is characterized by systemic inflammation caused by infection (Yanet al , 2019;Silveira et al , 2021). LPS as a potent inducer of excessive inflammatory response, is widely used to establish AKI and ALI mouse models, which exhibit similar pathological changes to those found in human infectious sepsis (Ju et al , 2018;Islam et al , 2019).…”

“…Epidemiological analysis has shown that sepsis is common pathology of AKI and ALI patients (Silveira et al , 2021). Sepsis is manifested by systemic inflammation, leading to multiple organs dysfunction, and the kidneys and lungs are the most vulnerable organs (Ibrahim et al , 2020).…”

“…Strategies targeting the exaggerated inflammatory response resulted in reduction of the damage severity of AKI and ALI. Anti-inflammatory drugs may be an effective option for the prevention of LPS-induced septic AKI and ALI (Chen et al , 2019;Silveira et al , 2021).…”

The Use of Standardized Brazilian Green Propolis Extract (EPP-AF) as an Adjunct Treatment for Hospitalized COVID-19 Patients (BeeCovid2): A Structured Summary of a Study Protocol for a Randomized Controlled Trial

“…Compounds derived from green propolis negatively regulate the expression of TMPRSS2 and the anchoring of ACE2, which limits entry of the virus [10,11]. Additionally, these compounds promote the immunomodulation of NF-κB, the NLRP3 in ammasome and monocytes/macrophages, reducing the overproduction of proin ammatory cytokines [1,6,7]. Experimental evidence also points to propolis substances capable of reducing activation of the PAK1 pathway, an important target used by the virus to shield itself from adaptive immunity [12].…”

“…Propolis is a natural resin with considerable evidence of antioxidant, antiviral, antiproteinuric, immunoregulatory and anti-in ammatory activities, and experimental data point to its potential actions against viral targets [1,[5][6][7].…”