We clarify the relation between the vertex operators in type IIB matrix model and superstring. Green-Schwarz light-cone closed superstring theory is obtained from IIB matrix model on two dimensional noncommutative backgrounds. Superstring vertex operators should be reproduced from those of IIB matrix model through this connection. Indeed, we confirm that supergravity vertex operators in IIB matrix model on the two dimensional backgrounds reduce to those in superstring theory. Noncommutativity plays an important role in our identification. Through this correspondence, we can reproduce superstring scattering amplitudes from IIB matrix model.