Green synthesis and anticancer activity of silver nanoparticles prepared using fruit extract of Azadirachta indica

Alharbi, Njud S.; Alsubhi, Nehad S.

doi:10.1016/j.jrras.2022.08.009

Cited by 33 publications

(14 citation statements)

References 54 publications

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“…The FT-IR analysis identified the specific functional groups and provided evidence of the involvement of phytochemicals in the reduction, capping, and stabilization of the Ag-NPs. These observed peaks collectively pointed toward the diverse functional groups associated with the synthesized Ag-NPs, consistent with previously reported findings. , The methanolic extract of B. nivosa is rich in flavonoids, phenols, saponins, terpenoids, and polyphenolics, which acted as capping agents, preventing the agglomeration and ensuring nanoparticle stability, which is crucial for maintaining the stability and uniformity of the synthesized Ag-NPs making them suitable for various applications.…”

Section: Discussionsupporting

confidence: 90%

“…These observed peaks collectively pointed toward the diverse functional groups associated with the synthesized Ag-NPs, consistent with previously reported findings. 38 , 71 The methanolic extract of B. nivosa is rich in flavonoids, phenols, saponins, terpenoids, and polyphenolics, which acted as capping agents, preventing the agglomeration and ensuring nanoparticle stability, which is crucial for maintaining the stability and uniformity of the synthesized Ag-NPs making them suitable for various applications. The FT-IR analysis further confirmed the presence of these secondary metabolites in the methanolic extract of B. nivosa , which play a significant role in the reduction, capping, and stabilization of Ag-NPs.…”

Section: Discussionmentioning

confidence: 99%

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Biosynthesis and Multifaceted Characterization of Breynia nivosa-Derived Silver Nanoparticles: An Eco-Friendly Approach for Biomedical Applications

Irshad,

Akash,

Rehman

et al. 2024

ACS Omega

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This study presents an environmentally friendly synthesis of stable silver nanoparticles (Ag-NPs) using the methanolic extract of Breynia nivosa. Initial phytochemical analysis of the extract revealed the presence of alkaloids, flavonoids, glycosides, saponins, and tannins. Further characterization through high-performance liquid chromatography (HPLC) and gas chromatography−mass spectrometry (GC-MS) analyses identified a diverse array of bioactive compounds, including hydroquinone, stearic acid, neophytadiene, 9,12-octadecadienoic acid (Z,Z), methyl ester, and others. The addition of B. nivosa methanolic extract to an AgNO 3 solution resulted in a color change, confirming the green synthesis of Ag-NPs through the reduction of AgNO 3 , as made evident by ultraviolet−visible (UV−vis) spectroscopy. X-ray diffraction (XRD) analysis provided valuable insights into the crystal structure, and scanning electron microscopy (SEM) analysis visualized the predominantly spherical shape of the Ag-NPs. However, the zeta (ζ)-potential and dynamic light scattering (DLS) analyses confirmed the stability and nanoscale dimensions of the synthesized Ag-NPs. Meanwhile, Fourier transform infrared (FT-IR) spectra exhibited peaks indicative of various functional groups, including carboxylic acids, phenols, alkanes, and isocyanates. These functional groups played a crucial role in both the reduction and capping processes of the Ag-NPs. The study further explored the antioxidant activity, cytotoxicity, acetylcholinesterase inhibition, and α-amylase inhibition activities of the Ag-NPs of the B. nivosa extract, demonstrating their potential for biomedical and therapeutic applications. In conclusion, this environmentally sustainable synthesis of Ag-NPs from the B. nivosa extract, enriched with bioactive secondary metabolites detected through HPLC and GC-MS analysis, holds promise for diverse applications in the burgeoning field of green nanotechnology.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Biosynthesis and Multifaceted Characterization of Breynia nivosa-Derived Silver Nanoparticles: An Eco-Friendly Approach for Biomedical Applications

Irshad,

Akash,

Rehman

et al. 2024

ACS Omega

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“… Saied E. et al (2023) , who created Ag-NPs, reported the same outcome and discovered they were spherical with a 17–35 nm diameter. Alharbi and Alsubhi (2022) , synthesized Ag-NPs with TEM imaging that were spherical with diameters of 27 nm. Based on a study conducted by El-fallal et al (2022) , the TEM analysis revealed that the AgNPs synthesized by Agrocybe cylindracea exhibited a spherical shape and a range of sizes from 3.47 to 13.99 nm.…”

Section: Resultsmentioning

confidence: 99%

“…The average size of the biosynthesized Ag-NPs, according to Saied E. et al (2023) , was 78 nm. The average diameter sizes of Ag-NPs and AgNPs-cis, using dynamic light scattering analysis, according to Alharbi and Alsubhi (2022) , were found to be 249 and 260 nm, respectively. The polydispersity index (PDI) value was used to determine whether the colloidal NPs were homogeneous or heterogeneous ( Soliman et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities

Saied,

Abdel-Maksoud,

Alfuraydi

et al. 2024

Front. Microbiol.

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In the current study, endophytic Aspergillus hiratsukae was used for the biosynthesis of silver nanoparticles (Ag-NPs) for the first time. The characterizations were performed using X ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and UV–Vis spectroscopy. The obtained results demonstrated the successful formation of crystalline, spherical Ag-NPs with particle diameters ranging from 16 to 31 nm. The FT-IR studied and displayed the various functional groups involved, which played a role in capping and reducing agents for Ag-NPs production. The SEM–EDX revealed that the main constituent of the AS-formed sample was primarily Ag, with a weight percentage of 64.2%. The mycosynthesized Ag-NPs were assessed for antimicrobial as well as photocatalytic activities. The antimicrobial results indicated that the synthesized Ag-NPs possess notable antibacterial efficacy against Staphylococcus aureus, Bacillus subtilis, and Escherichia coli, with minimum inhibitory concentrations (MICs) of Ag-NPs ranging from 62.5 to 250 μg/mL. Moreover, the biosynthesized Ag-NPs demonstrated weak antifungal activity against Aspergillus brasiliensis and Candida albicans, with MICs of 500 and 1,000 μg/mL, respectively. In addition, the mycosynthesized Ag-NPs exhibited photocatalytic activity toward acid black 2 (nigrosine) dye under both light and dark stimulation. Notably, After 300 min exposure to light, the nigrosine dye was degraded by 93%. In contrast, 51% degradation was observed after 300 min in darkness. In conclusion, Ag-NPs were successfully biosynthesized using endophytic A. hiratsukae and also exhibited antimicrobial and photocatalytic activities that can be used in environmental applications.

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“…Studies have also shown that caspase-3, a key player in apoptosis, is known to cleave both cytoplasmic and nuclear substrates, ultimately leading to cell death by inducing DNA damage. The upregulation of the gene is appreciated in the cancer cells treated with AgNPs that activate apoptosis (23,24).…”

Section: Discussionmentioning

confidence: 99%

Solanum trilobatum leaf extract-derived silver nanoparticles downregulate the PI3K/AKT/mTOR signaling pathway and attenuate oral squamous cell carcinoma cell proliferation

Ganesan,

Kumar N,

Natarajan

2024

J Herbmed Pharmacol

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Introduction: Oral cancer’s aggressive nature poses a significant health risk, demanding timely diagnosis and effective treatment. Despite progress in conventional therapies like chemotherapy and surgery, their limitations drive the exploration of alternative strategies. This study assessed Solanum trilobatum-derived silver nanoparticles (AgNPs) in impacting cancer cell cycles, inducing apoptosis, and modulating key pathways, including the phosphoinositide 3-kinase (PI3K)/Protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway, leveraging phytotherapy and nanotechnology—a promising frontier in cancer treatment. Methods: AgNPs were synthesized through the reduction of ions and stabilized using aqueous leaf extracts of S. trilobatum. After the characterization of AgNPs, the mRNA Gene Expression and mitochondrial membrane potentials (MMPs) were assessed. DNA fragmentation was done and DNA pattern by gel documentation system was observed. The study also assessed the modulation of the PI3K/Akt/mTOR cascade, impacting tumor growth and proliferation. Results: Biosynthesized AgNPs were characterized using UV-visible spectrophotometry (UVvis), energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), and Fourier-transform infrared (FTIR) spectroscopy. DNA fragmentation exhibited a typical ladder pattern. Dose-dependent changes in MMP were observed in the treated oral cancer cells. The effect of S. trilobatum-derived AgNPs in targeting the cell signaling pathway correlated significantly with their anticancer potency (P<0.001). Conclusion: This study reveals S. trilobatum leaf extract-based AgNPs as a natural cytostatic agent against oral squamous carcinoma. Utilizing nature’s resources and nanoscale science, they hold promise for enhancing oral cancer treatment outcomes and survival rates.

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Green synthesis and anticancer activity of silver nanoparticles prepared using fruit extract of Azadirachta indica

Cited by 33 publications

References 54 publications

Biosynthesis and Multifaceted Characterization of Breynia nivosa-Derived Silver Nanoparticles: An Eco-Friendly Approach for Biomedical Applications

Biosynthesis and Multifaceted Characterization of Breynia nivosa-Derived Silver Nanoparticles: An Eco-Friendly Approach for Biomedical Applications

Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities

Solanum trilobatum leaf extract-derived silver nanoparticles downregulate the PI3K/AKT/mTOR signaling pathway and attenuate oral squamous cell carcinoma cell proliferation

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